公开(公告)号：US06969763B1

公开(公告)日：2005-11-29

申请号：US09310844

申请日：1999-05-12

申请人： David J. Ecker ,  Richard Griffey ,  Stanley T. Crooke ,  Ranga Sampath ,  Eric Swayze ,  Venkatraman Mohan ,  Steven Hofstadler ,  John McNeil

发明人： David J. Ecker ,  Richard Griffey ,  Stanley T. Crooke ,  Ranga Sampath ,  Eric Swayze ,  Venkatraman Mohan ,  Steven Hofstadler ,  John McNeil

IPC分类号： C07H21/04 ,  C07K14/54 ,  C07K14/55 ,  C40B30/02 ,  C40B50/02 ,  G06F19/00

CPC分类号： C40B50/02 ,  C07H21/04 ,  C07K14/5406 ,  C07K14/55 ,  C40B30/02 ,  G06F19/14 ,  G06F19/16 ,  G06F19/28

摘要： Methods for the identification of compounds which modulate, either inhibit or stimulate, biomolecules are provided. Nucleic acids, especially RNAs are preferred substrates for such modulation. The present methods are particularly powerful in that they provide novel combinations of techniques which give rise to compounds, usually “small” organic compounds, which are highly potent modulators of RNA and other biomolecular activity. In accordance with preferred aspects of the invention, very large numbers of compounds may be tested essentially simultaneously to determine whether they are likely to interact with a molecular interaction site and modulate the activity of the biomolecule. Pharmaceuticals, veterinary drugs, agricultural chemicals, industrial chemicals, research chemicals and many other beneficial compounds may be identified in accordance with embodiments of this invention.

摘要翻译： 提供了用于鉴定调节，抑制或刺激生物分子的化合物的方法。 核酸，特别是RNA是用于这种调节的优选底物。 本发明的方法特别强大，因为它们提供了引起化合物（通常是“小”）有机化合物的技术的新组合，其是RNA和其它生物分子活性的高效调节剂。 根据本发明的优选方面，可以基本上同时测试非常大量的化合物以确定它们是否可能与分子相互作用位点相互作用并且调节生物分子的活性。 根据本发明的实施方案，可以鉴定药物，兽药，农药，工业化学品，研究化学品和许多其它有益化合物。

公开(公告)号：US06221587B1

公开(公告)日：2001-04-24

申请号：US09076440

申请日：1998-05-12

申请人： David J. Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

发明人： David J. Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

IPC分类号： C12Q168

CPC分类号： C12Q1/6809

摘要： Methods of identifying molecular interaction sites in eukaryotic and prokaryotic nucleic acids, especially RNA, are described. Secondary structural elements are identified from highly conserved sequences. Methods of preparing databases relating to such molecular interaction sites are also provided herein as are databases themselves. Therapeutic, agricultural, industrial, and other applicability results from interaction of such molecular interaction sites with “small” and other molecules.

摘要翻译： 描述了在真核和原核核酸，特别是RNA中鉴定分子相互作用位点的方法。 二级结构元件由高度保守的序列鉴定。 本文还提供了与这种分子相互作用位点相关的数据库的制备方法，数据库本身也是如此。 这种分子相互作用位点与“小”和其他分子的相互作用导致治疗，农业，工业和其他适用性。

公开(公告)号：US20050239737A1

公开(公告)日：2005-10-27

申请号：US11146468

申请日：2005-06-07

申请人： David Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

发明人： David Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

IPC分类号： C12N15/09 ,  A61K31/7105 ,  A61K31/713 ,  A61K48/00 ,  A61P31/04 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/6876

摘要： Methods of identifying molecular interaction sites in eukaryotic and prokaryotic nucleic acids, especially RNA, are described. Secondary structural elements are identified from highly conserved sequences. Methods of preparing databases relating to such molecular interaction sites are also provided herein as are databases themselves. Therapeutic, agricultural, industrial, and other applicability results from interaction of such molecular interaction sites with “small” and other molecules.

摘要翻译： 描述了在真核和原核核酸，特别是RNA中鉴定分子相互作用位点的方法。 二级结构元件由高度保守的序列鉴定。 本文还提供了与这种分子相互作用位点相关的数据库的制备方法，数据库本身也是如此。 这种分子相互作用位点与“小”和其他分子的相互作用导致治疗，农业，工业和其他适用性。