公开(公告)号：US06764648B1

公开(公告)日：2004-07-20

申请号：US09556897

申请日：2000-04-20

申请人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

发明人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

IPC分类号： G01N3500

CPC分类号： G01N27/44704 ,  B01L3/5027 ,  B01L99/00 ,  G01N27/44743 ,  G01N27/44782 ,  G01N27/44791 ,  G01N35/0099 ,  G01N35/1074 ,  G01N2035/00059 ,  G01N2035/00158 ,  G01N2035/0437 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/25 ,  Y10T436/2575

摘要： A substrate with a plurality of microchannels is movably deployed with other movable objects that will load sample into the microchannels, stimulate molecular migration, read the results of the migration, remove and replace the substrate, and prepare for a new run. The other objects include a gripper for engaging and moving the substrate, an electrode array of fine wires suitable for fitting into the microchannels for electromigration, and a scanning detector for reading migration results. A sequence of automatic operations is established so that one substrate after another may be moved into position, loaded with sample, stimulated for molecular migration, read with a beam, and then removed and replaced with a fresh substrate.

摘要翻译： 具有多个微通道的基板可移动地与其他可移动物体一起展开，其中可移动物体将样品载入微通道，刺激分子迁移，读取迁移结果，去除和替换基底，并准备新的运行。 其他目的包括用于接合和移动衬底的夹持器，适于安装到用于电迁移的微通道中的细线的电极阵列，以及用于读取迁移结果的扫描检测器。 建立了一系列自动操作，使得一个接一个的衬底可以移动到位置，装载样品，刺激分子迁移，用光束读取，然后移除并用新鲜的底物代替。

公开(公告)号：US06787111B2

公开(公告)日：2004-09-07

申请号：US09737675

申请日：2000-12-13

申请人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

发明人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

IPC分类号： G01N2726

CPC分类号： G01N27/44791 ,  B01J2219/0099 ,  B01L3/5027 ,  B01L99/00 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782 ,  G01N35/0099 ,  G01N35/1074 ,  G01N2035/00059 ,  G01N2035/00158 ,  G01N2035/0437

摘要： An apparatus for filling and cleaning channels and inlet ports of a microchip substrate is disclosed. A device of the apparatus comprising an array of tubes is inserted into each of the inlet ports of the microchip. The array of tubes of the device comprises a plurality of pressure tubes, surrounded by a plurality of vacuum tubes. In conjunction with this, pressurized solutions such as matrix or wash are introduced into common openings on the microchip that provide a passage to microchannels of the microchip with the use of pressure tip injectors of the apparatus. As matrix or wash solutions are pumped through the common openings and microchannels of the microchip substrate, wash solutions are pumped through the plurality of pressure tubes and everything is vacuumed through the plurality of vacuum tubes surrounding the plurality of pressure tubes. Various reservoirs of solutions are selected and allowed to flow by proper valve actuation. This process can be performed manually or easily automated by utilizing appropriate valves and control hardware/software.

摘要翻译： 公开了一种用于填充和清洁微芯片衬底的通道和入口的装置。 将包括管阵列的装置的装置插入到微芯片的每个入口端口中。 该装置的管阵列包括由多个真空管包围的多个压力管。 与此同时，诸如基质或洗涤剂的加压溶液被引入到微芯片上的共同的开口中，通过使用该装置的压头喷射器提供通向微芯片的微通道的通道。 当将基质或洗涤溶液泵送通过微芯片衬底的共同的开口和微通道时，将洗涤溶液泵送通过多个压力管，并且通过围绕多个压力管的多个真空管将一切都抽真空。 选择各种储存的溶液并通过适当的阀驱动使其流动。 该过程可以通过使用适当的阀门和控制硬件/软件手动或容易地进行自动化。

公开(公告)号：US06627446B1

公开(公告)日：2003-09-30

申请号：US09109676

申请日：1998-07-02

申请人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

发明人： David J. Roach ,  Robert T. Loder, Jr. ,  Thomas M. Armstrong ,  Dennis W. Harris ,  Stevan B. Jovanovich ,  Richard F. Johnston

IPC分类号： G01N2726

CPC分类号： G01N27/44704 ,  B01L3/5027 ,  B01L99/00 ,  G01N27/44743 ,  G01N27/44782 ,  G01N27/44791 ,  G01N35/0099 ,  G01N35/1074 ,  G01N2035/00059 ,  G01N2035/00158 ,  G01N2035/0437 ,  Y10T436/11 ,  Y10T436/110833 ,  Y10T436/25 ,  Y10T436/2575

摘要： A substrate with a plurality of microchannels is movably deployed with other movable objects that will load sample into the microchannels, stimulate molecular migration, read the results of the migration, remove and replace the substrate, and prepare for a new run. The other objects include a gripper for engaging and moving the substrate, an electrode array of fine wires suitable for fitting into the microchannels for electromigration, and a scanning detector for reading migration results. A sequence of automatic operations is established so that one substrate after another may be moved into position, loaded with sample, stimulated for molecular migration, read with a beam, and then removed and replaced with a fresh substrate.

摘要翻译： 具有多个微通道的基板可移动地与其他可移动物体一起展开，其中可移动物体将样品载入微通道，刺激分子迁移，读取迁移结果，去除和替换基底，并准备新的运行。 其他目的包括用于接合和移动衬底的夹持器，适于安装到用于电迁移的微通道中的细线的电极阵列，以及用于读取迁移结果的扫描检测器。 建立了一系列自动操作，使得一个接一个的衬底可以移动到位置，装载样品，刺激分子迁移，用光束读取，然后移除并用新鲜的底物代替。