公开(公告)号：US20070066851A1

公开(公告)日：2007-03-22

申请号：US11205493

申请日：2005-08-17

申请人： David Liu ,  Matthew Kanan ,  Mary Rozenman

发明人： David Liu ,  Matthew Kanan ,  Mary Rozenman

IPC分类号： C07C45/61

CPC分类号： C07C45/28 ,  C07B37/04 ,  C07B37/10 ,  C07C45/34 ,  C07C231/12 ,  C07D273/00 ,  C07C233/20 ,  C07C235/78

摘要： A novel palladium-mediated carbon-carbon bond forming reaction has been discovered using DNA-templated chemistry. The inventive reaction involves the palladium-mediated coupling of a terminal alkyne with an alkene to form an enone. A catalytic amount of palladium may be used in the reaction if an oxidant is present. The reactions is also compatible with a variety of organic solvent as well as aqueous solution. Both intermolecular and intramolecular reactions have been demonstrated. This novel carbon-carbon bond forming reaction is particularly useful in the synthesis of macrocycles. Kits, reagents, catalysts, solvents, oxidants, salts, acids, instructions, and other materials useful in the practice of the inventive reaction are also provided.

摘要翻译： 已经使用DNA模板化学发现了新的钯介导的碳 - 碳键形成反应。 本发明的反应涉及钯介导的末端炔与烯烃的偶联以形成烯酮。 如果存在氧化剂，催化量的钯可用于反应。 这些反应也与各种有机溶剂以及水溶液相容。 已经证明了分子间和分子内的反应。 这种新的碳 - 碳键形成反应在大环化合物的合成中特别有用。 还提供了用于实施本发明反应的试剂盒，试剂，催化剂，溶剂，氧化剂，盐，酸，说明书和其它材料。

公开(公告)号：US20050233381A1

公开(公告)日：2005-10-20

申请号：US11141164

申请日：2005-05-31

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

摘要翻译： 自然会通过迭代多样化，选择和扩增进化生物分子，如蛋白质。 本发明提供了基于核酸模板合成，选择，扩增和演化非天然分子的方法，组合物和系统。 核酸模板的序列用于引导非天然分子如非天然聚合物和小分子的合成。 使用这种方法可以制备和筛选这些分子的组合文库。 在选择分子时，其编码核酸模板可以被扩增和/或进化以产生用于重新筛选的相同分子或相关分子。 本发明的本发明的方法和组合物以与天然生物聚合物如多核苷酸和蛋白质的扩增类似的方式允许非天然分子的扩增和进化。

公开(公告)号：US20050227281A1

公开(公告)日：2005-10-13

申请号：US11141542

申请日：2005-05-31

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  C12Q1/68 ,  C08G63/48 ,  G01N33/53

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

公开(公告)号：US20050025766A1

公开(公告)日：2005-02-03

申请号：US10744605

申请日：2003-12-23

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  A61K39/395

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

公开(公告)号：US20050281819A9

公开(公告)日：2005-12-22

申请号：US10744605

申请日：2003-12-23

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  A61K39/395

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

公开(公告)号：US20050170376A1

公开(公告)日：2005-08-04

申请号：US10950367

申请日：2004-09-24

申请人： David Liu ,  Zev Gartner ,  Jeffrey Doyon ,  Christopher Calderone ,  Matthew Kanan ,  Xiaoyu Li ,  Thomas Snyder ,  Daniel Rosenbaum

发明人： David Liu ,  Zev Gartner ,  Jeffrey Doyon ,  Christopher Calderone ,  Matthew Kanan ,  Xiaoyu Li ,  Thomas Snyder ,  Daniel Rosenbaum

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12P41/00 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/68 ,  C12N15/1068

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The power of Nature and the flexibility of organic synthesis are combined in nucleic acid-templated synthesis. The present invention provides a variety of template architectures for performing nucleic acid-templated synthesis, methods for increasing the selectivity of nucleic acid-templated reactions, methods for performing stereoselective nucleic acid-templated reactions, methods of selecting for reaction products resulting from nucleic acid-templated synthesis, and methods of identifying new chemical reactions based on nucleic acid-templated synthesis.

摘要翻译： 自然会通过迭代多样化，选择和扩增进化生物分子，如蛋白质。 自然的力量和有机合成的灵活性结合在核酸模板合成中。 本发明提供用于进行核酸模板合成的多种模板结构，用于增加核酸模板化反应的选择性的方法，用于进行立体选择性核酸模板化反应的方法，选择由核酸 - 模板合成，以及基于核酸模板合成鉴定新化学反应的方法。

公开(公告)号：US20050142583A1

公开(公告)日：2005-06-30

申请号：US10949163

申请日：2004-09-24

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.

摘要翻译： 自然会通过迭代多样化，选择和扩增进化生物分子，如蛋白质。 本发明提供了基于核酸模板合成，选择，扩增和演化非天然分子的方法，组合物和系统。 核酸模板的序列用于引导非天然分子如非天然聚合物和小分子的合成。 使用这种方法可以制备和筛选这些分子的组合文库。 在选择分子时，其编码核酸模板可以被扩增和/或进化以产生用于重新筛选的相同分子或相关分子。 本发明的本发明的方法和组合物以与天然生物聚合物如多核苷酸和蛋白质的扩增类似的方式允许非天然分子的扩增和进化。

公开(公告)号：US20050042669A1

公开(公告)日：2005-02-24

申请号：US10949162

申请日：2004-09-24

申请人： David Liu ,  Zev Gartner ,  Matthew Kanan

发明人： David Liu ,  Zev Gartner ,  Matthew Kanan

IPC分类号： C12N15/09 ,  C07B61/00 ,  C08G85/00 ,  C12N15/10 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00 ,  C12Q1/70 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12N15/1068 ,  C12P1/00 ,  C12P19/34 ,  C12P21/00

摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.