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公开(公告)号：US4948975A
公开(公告)日：1990-08-14
申请号：US241992
申请日：1988-09-08
CPC分类号： G01N21/76
摘要： The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.
摘要翻译： QLIS图像和量化电磁场中的低水平化学发光反应。 它能够进行实时的非干扰测量和同时记录许多生化和化学反应，如发光免疫测定或酶测定。 该系统包括图像传输光学器件，具有图像增强微通道板的低光级数字化相机，图像处理或控制计算机。 图像传输光学器件可以是具有弯曲或显微镜的光纤图像引导件，以将光照射到RF场外。 相机的输出变换为累积数字化数据或增强型视频显示或硬拷贝图像的本地化速率。 该系统可以用作射频或微波辐射的发光微量测量装置，作为热剂量计，或者用于超声（声发光）或电离辐射的剂量测定。 它提供了一种近实时系统，能够在化学发光测定和热微量测定领域中测量电磁场中的发光反应的极低的光照水平，并且能够近似实时成像样品以允许空间分布 分析反应。 它可用于测量三种明显不同的照射配置，包括（1）小培养皿样品池的RF波导照射，（2）用于显微镜成像和测量的样品的RF照射，（3） ）在消声室中对人体大小的样品进行射频照射。
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公开(公告)号：US06303316B1
公开(公告)日：2001-10-16
申请号：US09608706
申请日：2000-06-30
申请人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
发明人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , Y10T436/143333 , C12Q2565/607
摘要： In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.
摘要翻译： 在识别复合体系中，包含随机DNA序列的核酸配体可操作地偶联到有机半导体上，并分布以形成识别复合物的阵列。 当将未知的化学或生物分析物施加到阵列时，一个或多个识别复合物的电和/或光化学性质在核酸配体与分析物结合时被改变。 电和/或光化学性质变化的程度是核酸配体序列对分析物的亲和力的函数。 与阵列相关联的电学和光化学变化作为一个整体，可以用作识别分析物的独特标记。 在某些实施方案中，结合分析物的核酸配体的选择和扩增的迭代过程可用于产生对靶分析物具有更大亲和性和特异性的新阵列，或产生具有高结合力的一个或多个核酸配体 对分析物的亲和力。 本发明还提供了制备以高亲和力结合分析物并使用这种核酸配体中和分析物的核酸配体的方法。
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公开(公告)号：US4956558A
公开(公告)日：1990-09-11
申请号：US324086
申请日：1989-03-16
CPC分类号： G01B11/0658 , F16N29/00
摘要： A system for determining the thicknesses of thin films of materials exhibiting fluorescence in response to exposure to excitation energy from a suitable source of such energy. A section of film is illuminated with a fixed level of excitation energy from a source such as an argon ion laser emitting blue-green light. The amount of fluorescent light produced by the film over a limited area within the section so illuminated is then measured using a detector such as a photomultiplier tube. Since the amount of fluorescent light produced is a function of the thicknesses of thin films, the thickness of a specific film can be determined by comparing the intensity of fluorescent light produced by this film with the intensity of light produced by similar films of known thicknesses in response to the same amount of excitation energy. The preferred embodiment of the invention uses fiber optic probes in measuring the thicknesses of oil films on the operational components of machinery which are ordinarily obscured from view.
摘要翻译： 用于确定响应于从这种能量的合适来源暴露于激发能量而显示荧光的材料的薄膜的厚度的系统。 用来自诸如发射蓝绿光的氩离子激光器的源的固定水平的激发能量照射一部分膜。 然后使用诸如光电倍增管的检测器来测量在如此照射的部分内的限制区域上由膜产生的荧光量。 由于产生的荧光量是薄膜的厚度的函数，所以可以通过将由该膜产生的荧光的强度与已知厚度的类似膜产生的光的强度进行比较来确定特定膜的厚度 响应相同量的激发能量。 本发明的优选实施例使用光纤探针来测量机械操作部件上油膜的厚度，这些部件通常从视野中被遮蔽。
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