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公开(公告)号：US5658673A
公开(公告)日：1997-08-19
申请号：US504306
申请日：1995-07-20
CPC分类号： C03C17/3405 , G01R29/0857 , C03C2217/42 , C03C2217/475 , G01R29/0878 , Y10T428/31511 , Y10T428/31515 , Y10T428/31522 , Y10T428/31525 , Y10T428/31529 , Y10T428/31551 , Y10T428/31591 , Y10T428/31605 , Y10T428/31609 , Y10T428/31725 , Y10T428/31768 , Y10T428/31772 , Y10T428/31775 , Y10T428/31779 , Y10T428/31783 , Y10T428/31844 , Y10T428/31848
摘要： An article which is sensitive to non-ionizing electromagnetic radiation in the radiofrequency radiation range, consisting essentially of a substrate, a layer of a binder material and a layer of activated diazoluminomelanin. This article is prepared by coating at least one surface of a substrate with a binder, immersing the binder-coated substrate into a solution comprising luminol, 3AT and a soluble nitrite for a period of about 2 to 12 days to provide a layer of diazoluminomelanin (DALM) on the binder layer, removing the DALM-coated film from the solution and rinsing the same, activating the DALM with sodium bicarbonate and hydrogen peroxide for a period of about 2 to 12 days.
摘要翻译： 在射频辐射范围内对非电离电磁辐射敏感的物品，其基本上由基底，粘合剂材料层和活化的二叠氮木兰素层组成。 本文通过用粘合剂涂覆基材的至少一个表面来制备，将粘合剂涂覆的基材浸入包含鲁米诺3AT和可溶性亚硝酸盐的溶液中约2至12天的时间以提供一层二叠氮木兰宁（ DALM），从溶液中除去DALM涂膜并漂洗，用碳酸氢钠和过氧化氢活化DALM约2-12天。
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公开(公告)号：US5374811A
公开(公告)日：1994-12-20
申请号：US879015
申请日：1992-05-06
CPC分类号： H05B6/806 , A61M5/445 , H05B6/642 , H05B6/645 , A61M2205/362 , A61M2205/3686
摘要： An improved method and apparatus for safely and rapidly rewarming large quantities of frozen or refrigerated blood combines microwave, or radio frequency radiation, heating with a forced air system. Forced air is flowed over units of blood or tissue from those units that will absorb the most microwave energy, generally those units closest to the source of microwave energy, to those units that will absorb the least microwave energy, generally those units furthest from the source of microwave energy. The forced air initially thaws the closest units so that they begin to absorb significant microwave energy. The forced air, preferably chilled, then prevents overheating of those closest units by transferring heat to more distant units, beginning their thawing and contributing to their rewarming. The microwave energy source is turned off when the output temperature of the forced air reaches a preselected temperature and the flow of forced air continues until the output temperature stabilizes. The apparatus includes a separated stack of perforated plates, each plate holding two units, mounted inside a microwave oven. Forced air drives a turbine rotor which rotates the plates while the air leaving the turbine flows down among the stacked plates and out the microwave oven.
摘要翻译： 一种用于安全和快速地重新加热大量冷冻或冷藏血液的改进方法和装置将微波或射频辐射，强制空气系统加热。 强制空气从那些将吸收最微波能量的微波能量（通常是最接近微波能量源的单元）流向血液或组织的单位，到达吸收最少微波能量的那些单元，通常是离源最远的单元 的微波能量。 强制空气最初解冻最近的单位，以便它们开始吸收显着的微波能量。 强制空气，优选冷冻，然后通过将热量传递到更远的单元，开始解冻并有助于它们的再温暖，从而防止最近的单元过热。 当强制空气的输出温度达到预选温度并强制空气流持续直到输出温度稳定时，微波能量源关闭。 该装置包括分开的多孔板堆叠，每个板保持两个单元，安装在微波炉内。 强制空气驱动涡轮转子，涡轮转子旋转板，同时离开涡轮机的空气在堆叠的板之间向下流动并流出微波炉。
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公开(公告)号：US4948975A
公开(公告)日：1990-08-14
申请号：US241992
申请日：1988-09-08
CPC分类号： G01N21/76
摘要： The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.
摘要翻译： QLIS图像和量化电磁场中的低水平化学发光反应。 它能够进行实时的非干扰测量和同时记录许多生化和化学反应，如发光免疫测定或酶测定。 该系统包括图像传输光学器件，具有图像增强微通道板的低光级数字化相机，图像处理或控制计算机。 图像传输光学器件可以是具有弯曲或显微镜的光纤图像引导件，以将光照射到RF场外。 相机的输出变换为累积数字化数据或增强型视频显示或硬拷贝图像的本地化速率。 该系统可以用作射频或微波辐射的发光微量测量装置，作为热剂量计，或者用于超声（声发光）或电离辐射的剂量测定。 它提供了一种近实时系统，能够在化学发光测定和热微量测定领域中测量电磁场中的发光反应的极低的光照水平，并且能够近似实时成像样品以允许空间分布 分析反应。 它可用于测量三种明显不同的照射配置，包括（1）小培养皿样品池的RF波导照射，（2）用于显微镜成像和测量的样品的RF照射，（3） ）在消声室中对人体大小的样品进行射频照射。
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公开(公告)号：US5089385A
公开(公告)日：1992-02-18
申请号：US521064
申请日：1990-05-07
IPC分类号： C12M3/00
CPC分类号： C12M41/12 , C12M29/10 , C12M29/26 , Y10S435/813
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译： 描述了流通细胞培养装置和方法。 流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。 流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。 流通池还包括用于接收热探针的卡盘。 探头由电磁非交互材料制成。 流通池封闭在流通池支架内。 流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。 空腔具有粗糙的壁，以促进湍流。 流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。 单向阀防止液体营养物质通过进气口流出室外。 通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。 一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。 加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。 流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。
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公开(公告)号：US5028541A
公开(公告)日：1991-07-02
申请号：US56034
申请日：1987-06-01
IPC分类号： C12M3/00
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译： 描述了流通细胞培养装置和方法。 流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。 流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。 流通池还包括用于接收热探针的卡盘。 探头由电磁非交互材料制成。 流通池封闭在流通池支架内。 流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。 空腔具有粗糙的壁，以促进湍流。 流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。 单向阀防止液体营养物质通过进气口流出室外。 通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。 一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。 加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。 流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。
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