公开(公告)号：US11796535B2

公开(公告)日：2023-10-24

申请号：US16332271

申请日：2016-11-21

申请人： Plexbio Co., Ltd. ,  Dean Tsao

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Yao-Kuang Chung

IPC分类号： G01N33/543 ,  G01N33/545 ,  C08L63/00

CPC分类号： G01N33/54333 ,  G01N33/545 ,  C08L63/00

摘要： Disclosed herein are compositions comprising beads with unique analog code identifiers for storing information about a multiplex assay as well as methods for using the same in multiplex chemical and biological assays.

公开(公告)号：US10302640B2

公开(公告)日：2019-05-28

申请号：US15374930

申请日：2016-12-09

申请人： PLEXBIO CO., LTD.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Yao-Kuang Chung

IPC分类号： G01N33/553 ,  G01N33/545 ,  G01N33/543 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6834

摘要： Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

公开(公告)号：US20180195113A1

公开(公告)日：2018-07-12

申请号：US15836809

申请日：2017-12-08

申请人： PLEXBIO CO., LTD.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Shian pin Hu

IPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6816 ,  C12Q1/6834

摘要： Provided herein are methods and kits for detecting the presence of DNA mutations in the KRAS, BRAF, CTNNB1, and APC genes. The methods and kits employ microcarriers, each with a probe specific for a DNA mutation in the KRAS, BRAF, CTNNB1, or APC gene and an identifier unique to the probe sequence. Upon isolation and amplification of DNA from a sample, hybridization of amplified DNA with a probe, specific for a DNA mutation, that is coupled to a microcarrier indicates the presence of the DNA mutation in the sample. Since each microcarrier can be identified through detection of the identifier, multiplex screening assays for multiple mutations in each of the KRAS, BRAF, CTNNB1, and APC genes are provided.

公开(公告)号：US12099011B2

公开(公告)日：2024-09-24

申请号：US17083182

申请日：2020-10-28

申请人： PLEXBIO CO., LTD.

发明人： Cheng-Tse Lin ,  Yi-Ming Sun ,  Kuei-Shen Hsu ,  Liang-Han Chang ,  Yao-Kuang Chung ,  Chin-Yun Wu ,  Chin-Shiou Huang

IPC分类号： G01N21/64 ,  B01L3/00 ,  C08F20/18 ,  C08F120/14 ,  C08K5/357 ,  C09B69/10 ,  C09K11/02 ,  C09K11/06 ,  G01N21/27 ,  G01N21/93

CPC分类号： G01N21/6452 ,  B01L3/5085 ,  C08F20/18 ,  C08F120/14 ,  C08K5/357 ,  C09B69/101 ,  C09B69/103 ,  C09B69/105 ,  C09B69/109 ,  C09K11/02 ,  C09K11/06 ,  G01N21/278 ,  G01N21/6486 ,  B01L2200/12 ,  B01L2200/148 ,  B01L2300/0829 ,  C09K2211/1033 ,  G01N2201/127

摘要： The present invention relates to a fluorescent polymer cast on the surface of wells of a plate and a plate comprising the fluorescent polymer. The plate is used in a method for calibrating read-out values of plate readers. The method for calibrating the plate readers can effectively reduce the deviation of read-out values among different plate readers.

公开(公告)号：US11579522B2

公开(公告)日：2023-02-14

申请号：US16386092

申请日：2019-04-16

申请人： Plexbio Co., Ltd.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Yao-Kuang Chung

IPC分类号： G03F1/80 ,  G01N33/543 ,  C12Q1/6813 ,  G02F1/1362 ,  G09G3/36 ,  H01L27/12 ,  B01L3/00 ,  G03F7/004 ,  G03F7/00 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/545 ,  G01N33/553

摘要： Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

公开(公告)号：US10894975B2

公开(公告)日：2021-01-19

申请号：US15838331

申请日：2017-12-11

申请人： PLEXBIO CO., LTD.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Shian pin Hu

IPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6816 ,  G01N21/64 ,  C12Q1/6834

摘要： Provided herein are methods and kits for detecting the presence of DNA mutations in the KRAS, BRAF, CTNNB1, and APC genes. The methods and kits employ microcarriers, each with a probe specific for a DNA mutation in the KRAS, BRAF, CTNNB1, or APC gene and an identifier unique to the probe sequence. Upon isolation and amplification of DNA from a sample, hybridization of amplified DNA with a probe, specific for a DNA mutation, that is coupled to a microcarrier indicates the presence of the DNA mutation in the sample. Since each microcarrier can be identified through detection of the identifier, multiplex screening assays for multiple mutations in each of the KRAS, BRAF, CTNNB1, and APC genes are provided.

公开(公告)号：US10859910B2

公开(公告)日：2020-12-08

申请号：US16386087

申请日：2019-04-16

申请人： Plexbio Co., Ltd.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Yao-Kuang Chung

IPC分类号： G03F1/80 ,  G01N33/543 ,  C12Q1/6813 ,  G02F1/1362 ,  G09G3/36 ,  H01L27/12 ,  B01L3/00 ,  G03F7/004 ,  G03F7/00 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/545 ,  G01N33/553

摘要： Provided herein are encoded microcarriers for analyte detection in multiplex assays. The microcarriers are encoded with an analog code for identification and include a capture agent for analyte detection. Also provided are methods of making the encoded microcarriers disclosed herein. Further provided are methods and kits for conducting a multiplex assay using the microcarriers described herein.

公开(公告)号：US10436778B2

公开(公告)日：2019-10-08

申请号：US14208481

申请日：2014-03-13

申请人： PLEXBIO CO., LTD.

发明人： Dean Tsao ,  Chin-Shiou Huang

IPC分类号： G01N33/543 ,  C40B30/04 ,  C40B40/04 ,  C40B50/16

摘要： Disclosed herein are compositions comprising beads with unique identifiers for storing information about a multiplex assay as well as methods for using the same in multiplex chemical and biological assays.

公开(公告)号：US20180201983A1

公开(公告)日：2018-07-19

申请号：US15838331

申请日：2017-12-11

申请人： PLEXBIO CO., LTD.

发明人： Dean Tsao ,  Chin-Shiou Huang ,  Shian pin Hu

IPC分类号： C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N21/64

CPC分类号： C12Q1/6827 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/16 ,  G01N21/6428 ,  G01N2021/6439

摘要： Provided herein are methods and kits for detecting the presence of DNA mutations in the KRAS, BRAF, CTNNB1, and APC genes. The methods and kits employ microcarriers, each with a probe specific for a DNA mutation in the KRAS, BRAF, CTNNB1, or APC gene and an identifier unique to the probe sequence. Upon isolation and amplification of DNA from a sample, hybridization of amplified DNA with a probe, specific for a DNA mutation, that is coupled to a microcarrier indicates the presence of the DNA mutation in the sample. Since each microcarrier can be identified through detection of the identifier, multiplex screening assays for multiple mutations in each of the KRAS, BRAF, CTNNB1, and APC genes are provided.