公开(公告)号：US20100028965A1

公开(公告)日：2010-02-04

申请号：US11916180

申请日：2006-06-01

申请人： Dehua Liu ,  hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18

CPC分类号： C12P7/18 ,  C12N1/32

摘要： The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol.

摘要翻译： 本发明公开了一种1,3-丙二醇的制造方法，其特征在于，在生物柴油的生产中使用副产物粗甘油，无需进一步处理，作为1,3-丙二醇的生产用基材。 在含有来自生物柴油生产的副产物的粗甘油的种子培养基中接种1,3-丙二醇生产菌株; 将种子培养物加入到含有来自生物柴油生产的副产物和发酵的粗甘油的发酵培养基中; 保持pH在6.8至8.0的范围内; 并在发酵结束时，分离和纯化1,3-丙二醇。

公开(公告)号：US08486673B2

公开(公告)日：2013-07-16

申请号：US11916180

申请日：2006-06-01

申请人： Dehua Liu ,  Hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  Hongjuan Liu ,  Yan Sun ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18

CPC分类号： C12P7/18 ,  C12N1/32

摘要： The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol.

摘要翻译： 本发明公开了一种1,3-丙二醇的制造方法，其特征在于，在生物柴油的生产中使用副产物粗甘油，无需进一步处理，作为1,3-丙二醇的生产用基材。 在含有来自生物柴油生产的副产物的粗甘油的种子培养基中接种1,3-丙二醇生产菌株; 将种子培养物加入到含有来自生物柴油生产的副产物和发酵的粗甘油的发酵培养基中; 保持pH在6.8至8.0的范围内; 并在发酵结束时，分离和纯化1,3-丙二醇。

公开(公告)号：US07968319B2

公开(公告)日：2011-06-28

申请号：US11917682

申请日：2006-06-13

申请人： Dehua Liu ,  Keke Cheng ,  Hongjuan Liu ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  Keke Cheng ,  Hongjuan Liu ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18 ,  C12P39/00

CPC分类号： C12P7/18

摘要： The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g/L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g/L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.

摘要翻译： 本发明公开了一种生淀粉材料制备1,3-丙二醇和2,3-丁二醇的方法，包括以下步骤：1）将假丝酵母或汉逊酵母阿拉伯糖醇菌接种到发酵培养基中，将原料淀粉的糖化液 作为碳源; 酵母细胞在需氧条件下培养，直到葡萄糖消耗率显着降低，然后厌氧发酵至葡萄糖浓度为5至10g / L; 收集发酵液并过滤，除去培养液中的酵母细胞，得到的滤液为甘油发酵液; 2）将克雷伯氏菌，丁酸梭菌或巴斯德氏梭菌接种到发酵培养基中，其中从步骤1）获得的甘油发酵液用作碳源; 细菌厌氧发酵30-32小时，当1,3-丙二醇的生成速率明显降低时有氧发酵，当甘油浓度降至10 g / L以下时，发酵停止， 最后得到1,3-丙二醇和2,3-丁二醇。 本发明的方法可以有效地降低生产成本并提高生产率。

公开(公告)号：US20090081745A1

公开(公告)日：2009-03-26

申请号：US11917682

申请日：2006-06-13

申请人： Dehua Liu ,  Keke Cheng ,  Hongjuan Liu ,  Rihui Lin ,  Jian Hao

发明人： Dehua Liu ,  Keke Cheng ,  Hongjuan Liu ,  Rihui Lin ,  Jian Hao

IPC分类号： C12P7/18

CPC分类号： C12P7/18

摘要： The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g/L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g/L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.

摘要翻译： 本发明公开了一种生淀粉材料制备1,3-丙二醇和2,3-丁二醇的方法，包括以下步骤：1）将假丝酵母或汉逊酵母阿拉伯糖醇菌接种到发酵培养基中，将原料淀粉的糖化液 作为碳源; 酵母细胞在需氧条件下培养，直到葡萄糖消耗率显着降低，然后厌氧发酵至葡萄糖浓度为5至10g / L; 收集发酵液并过滤，除去培养液中的酵母细胞，得到的滤液为甘油发酵液; 2）将克雷伯氏菌，丁酸梭菌或巴斯德氏梭菌接种到发酵培养基中，其中从步骤1）获得的甘油发酵液用作碳源; 细菌厌氧发酵30-32小时，当1,3-丙二醇的生成速率明显降低时有氧发酵，当甘油浓度降至10 g / L以下时，发酵停止， 最后得到1,3-丙二醇和2,3-丁二醇。 本发明的方法可以有效地降低生产成本并提高生产率。

公开(公告)号：US09081998B2

公开(公告)日：2015-07-14

申请号：US13699116

申请日：2011-05-09

申请人： Jian Hao ,  Fan Zhang ,  Maosheng Dong ,  Yi Luo ,  Sheng Wu ,  Yongfei Zhao ,  Bo Wang

发明人： Jian Hao ,  Fan Zhang ,  Maosheng Dong ,  Yi Luo ,  Sheng Wu ,  Yongfei Zhao ,  Bo Wang

IPC分类号： G06K9/00 ,  G01N23/04

CPC分类号： G06K9/00127 ,  G01N23/04

摘要： This invention discloses a method for utilizing soft X-ray microimaging for cancer cell image recognition. The method comprises the steps of 1) sample preparation; 2) pathological examination; 3) soft X-ray imaging; and 4) analysis and recognition. This invention applies soft X-ray microimaging for cancer cell image recognition, successfully obtains the soft X-ray microscopic image of a cancer cell by scanning the cancer cell with synchrotron radiation soft X-ray microimaging, provides recognition steps and experimental data, and establishes a method for utilizing soft X-ray microimaging for cancer cell image recognition. This invention creates a method for analyzing soft X-ray microscopic images, provides a novel synchrotron radiation soft X-ray pathological diagnosis method for cancer diagnosis, and provides an extremely valuable basis for the creation and clinical application of soft X-ray pathology in the 21st century.

摘要翻译： 本发明公开了一种利用软X射线微成像进行癌细胞图像识别的方法。 该方法包括以下步骤：1）样品制备; 2）病理检查; 3）软X射线成像; 和4）分析和认可。 本发明应用软X射线微成像进行癌细胞图像识别，通过用同步辐射软X射线微成像扫描癌细胞，成功获得癌细胞的软X射线显微镜图像，提供识别步骤和实验数据，并建立 一种利用软X射线微成像进行癌细胞图像识别的方法。 本发明创建了一种分析软X射线显微镜图像的方法，为癌症诊断提供了一种新型的同步辐射软X射线病理诊断方法，为软X射线病理学的创造和临床应用提供了极其宝贵的基础 21世纪。

公开(公告)号：USD632940S1

公开(公告)日：2011-02-22

申请号：US29360899

申请日：2010-05-03

申请人： Yuji Yamamoto ,  Jian Hao

设计人： Yuji Yamamoto ,  Jian Hao

公开(公告)号：US20130071876A1

公开(公告)日：2013-03-21

申请号：US13699116

申请日：2011-05-09

申请人： Jian Hao ,  Fan Zhang ,  Maosheng Dong ,  Yi Luo ,  Sheng Wu ,  Yongfei Zhao ,  Bo Wang

发明人： Jian Hao ,  Fan Zhang ,  Maosheng Dong ,  Yi Luo ,  Sheng Wu ,  Yongfei Zhao ,  Bo Wang

IPC分类号： G01N23/04

CPC分类号： G06K9/00127 ,  G01N23/04

摘要： This invention discloses a method for utilizing soft X-ray microimaging for cancer cell image recognition. The method comprises the steps of 1) sample preparation; 2) pathological examination; 3) soft X-ray imaging; and 4) analysis and recognition. This invention applies soft X-ray microimaging for cancer cell image recognition, successfully obtains the soft X-ray microscopic image of a cancer cell by scanning the cancer cell with synchrotron radiation soft X-ray microimaging, provides recognition steps and experimental data, and establishes a method for utilizing soft X-ray microimaging for cancer cell image recognition. This invention creates a method for analyzing soft X-ray microscopic images, provides a novel synchrotron radiation soft X-ray pathological diagnosis method for cancer diagnosis, and provides an extremely valuable basis for the creation and clinical application of soft X-ray pathology in the 21st century.

摘要翻译： 本发明公开了一种利用软X射线微成像进行癌细胞图像识别的方法。 该方法包括以下步骤：1）样品制备; 2）病理检查; 3）软X射线成像; 和4）分析和认可。 本发明应用软X射线微成像进行癌细胞图像识别，通过用同步辐射软X射线微成像扫描癌细胞，成功获得癌细胞的软X射线显微镜图像，提供识别步骤和实验数据，并建立 一种利用软X射线微成像进行癌细胞图像识别的方法。 本发明创建了一种分析软X射线显微镜图像的方法，为癌症诊断提供了一种新型的同步辐射软X射线病理诊断方法，为软X射线病理学的创造和临床应用提供了极其宝贵的基础 21世纪。