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公开(公告)号：US20180057892A1
公开(公告)日：2018-03-01
申请号：US15259239
申请日：2016-09-08
发明人： Yong Zhang , Chih-Yu Chao , Wanyue Li , Ying-Ting Chen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6893
摘要： Primer pair, kit and method of detecting Babesia canis are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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公开(公告)号：US10513740B2
公开(公告)日：2019-12-24
申请号：US15678732
申请日：2017-08-16
发明人： Yong Zhang , Chih-Yu Chao
IPC分类号： C07H21/04 , C12Q1/68 , C12Q1/689 , C07K16/12 , G01N33/569
摘要： Primer pair, kit and method for detecting Anaplasma platys are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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公开(公告)号：US20170015992A1
公开(公告)日：2017-01-19
申请号：US14831164
申请日：2015-08-20
发明人： Revata Utama , Yong Zhang , Song Bin Huang , Wang Chu Chen
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12Q2527/101 , C12Q2527/119
摘要： A nucleic acid extraction method includes the following steps. Firstly, a mixture of a sample and a lysis buffer is provided. The pH of the lysis buffer is lower than 7. Then, the mixture of the sample and the lysis buffer is transferred to a nucleic acid catching chamber containing chitosan coating material, so that nucleic acid of the sample is bound to the chitosan coating material. Then, the chitosan coating material is washed with at least one wash buffer, thereby removing residual protein and cell debris. Then, the chitosan coating material is eluted with an elution buffer while heating the chitosan coating material, so that the nucleic acid is separated from the chitosan coating material. The pH of the elution buffer is higher than 7.
摘要翻译： 核酸提取方法包括以下步骤。 首先，提供样品和裂解缓冲液的混合物。 裂解缓冲液的pH低于7.然后，将样品和裂解缓冲液的混合物转移到含壳聚糖涂层材料的核酸捕获室中，使得样品的核酸与壳聚糖涂层材料结合。 然后，用至少一个洗涤缓冲液洗涤壳聚糖涂层材料，从而除去残留的蛋白质和细胞碎片。 然后，在加热壳聚糖涂层材料的同时，用洗脱缓冲液洗脱壳聚糖涂层材料，使得核酸与壳聚糖涂层材料分离。 洗脱缓冲液的pH高于7。
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公开(公告)号：US20190271048A1
公开(公告)日：2019-09-05
申请号：US16414672
申请日：2019-05-16
发明人： Yong Zhang , Chih-Yu Chao , Wanyue Li , Ying-Ting Chen
IPC分类号： C12Q1/6893
摘要： Primer pair, kit and method of detecting Babesia canis are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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公开(公告)号：US20180080066A1
公开(公告)日：2018-03-22
申请号：US15678802
申请日：2017-08-16
发明人： Yong Zhang , Chih-Yu Chao , Kah Sin Loh
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： Primer pair, kit and method for detecting Ehrlichia Canis are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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公开(公告)号：US20180073058A1
公开(公告)日：2018-03-15
申请号：US15678732
申请日：2017-08-16
发明人： Yong Zhang , Chih-Yu Chao
IPC分类号： C12Q1/68 , G01N33/569 , C07K16/12
CPC分类号： C12Q1/689 , C07K16/1246 , C12Q2600/16 , G01N33/56911 , G01N2333/29
摘要： Primer pair, kit and method for detecting Anaplasma platys are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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公开(公告)号：US20220145351A1
公开(公告)日：2022-05-12
申请号：US17520070
申请日：2021-11-05
发明人： Yong Zhang , Jia Hui Esther Boey , Weishi Zhang , Quanke Long , Jiaojiao Li , Xiaozhi Zhao , Kun-Nan Tsai
IPC分类号： C12Q1/06
摘要： A method for detecting infectivity of a human coronavirus is provided. The method includes steps of: (a) dividing a testing sample into a first sample and a second sample; (b) treating the first sample with an intercalating dye or chemical; (c) exposing the first sample to a light for photo-activation; (d) amplifying targeted nucleic acids in the first sample and the second sample; and (e) determining infectivity of the human coronavirus based on amplification results of the first sample and the second sample.
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公开(公告)号：US10316314B2
公开(公告)日：2019-06-11
申请号：US14831164
申请日：2015-08-20
发明人： Revata Utama , Yong Zhang , Song Bin Huang , Wang Chu Chen
IPC分类号： C12N15/10
摘要： A nucleic acid extraction method includes the following steps. Firstly, a mixture of a sample and a lysis buffer is provided. The pH of the lysis buffer is lower than 7. Then, the mixture of the sample and the lysis buffer is transferred to a nucleic acid catching chamber containing chitosan coating material, so that nucleic acid of the sample is bound to the chitosan coating material. Then, the chitosan coating material is washed with at least one wash buffer, thereby removing residual protein and cell debris. Then, the chitosan coating material is eluted with an elution buffer while heating the chitosan coating material, so that the nucleic acid is separated from the chitosan coating material. The pH of the elution buffer is higher than 7.
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公开(公告)号：US20190055611A1
公开(公告)日：2019-02-21
申请号：US15683987
申请日：2017-08-23
发明人： Yong Zhang , Chih-Yu Chao
IPC分类号： C12Q1/68
摘要： Primer pair, kit and method for detecting Babesia gibsoni are disclosed. The primer pair includes a forward primer and a reverse primer, and the kit includes the primer pair and a probe. The forward primer has a sequence of SEQ ID NO: 1, the reverse primer has a sequence of SEQ ID NO: 2, and the probe has a sequence of SEQ ID NO: 3.
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