公开(公告)号：US4256838A

公开(公告)日：1981-03-17

申请号：US93569

申请日：1979-11-13

申请人： Denise M. Jackson ,  Yoshihisa Tsuda ,  Vida Winans

发明人： Denise M. Jackson ,  Yoshihisa Tsuda ,  Vida Winans

IPC分类号： C12N9/00 ,  C12N9/92

CPC分类号： C12N9/92 ,  C12N9/00 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/827 ,  Y10S435/853 ,  Y10S435/886

摘要： A process for purifying an enzyme such as glucose isomerase which comprises precipitating nucleic acids from a cell-free, heat-treated enzyme solution in a suitable buffer and chromatographing the supernatant on a cellulosic medium. Subsequent chromatography on a hydrophilic, molecular-sieve medium affords enzyme of about 90% purity.

摘要翻译： 一种纯化诸如葡萄糖异构酶的酶的方法，其包括在合适的缓冲液中从无细胞的经热处理的酶溶液中沉淀核酸，并在纤维素介质上色谱分离上清液。 随后在亲水性分子筛介质上进行色谱提供约90％纯度的酶。

公开(公告)号：US4551432A

公开(公告)日：1985-11-05

申请号：US551646

申请日：1983-11-14

申请人： Yoshihisa Tsuda ,  Denise M. Jackson

发明人： Yoshihisa Tsuda ,  Denise M. Jackson

IPC分类号： C12N9/88 ,  C12P7/42 ,  C12N1/20 ,  C12R1/01

CPC分类号： C12N9/88 ,  C12P7/42 ,  Y10S435/822

摘要： A strain of Paracoccus denitrificans, NRRL B-15710, is an overproducer of a fumarase which is readily purified and relatively thermostable. The fumarase so produced converts fumaric acid to L-malic acid without formation of any significant amounts of a coproduct.

摘要翻译： 副反应芽孢杆菌NRRL B-15710的菌株是富马酸酯的过量产物，其易于纯化并相对热稳定。 如此生产的富马酸盐将富马酸转化为L-苹果酸，而不形成任何显着量的副产物。

公开(公告)号：US4237231A

公开(公告)日：1980-12-02

申请号：US93570

申请日：1979-11-13

申请人： Denise M. Jackson ,  Yoshihisa Tsuda

发明人： Denise M. Jackson ,  Yoshihisa Tsuda

IPC分类号： C12N9/92 ,  C12N9/96

CPC分类号： C12N9/92 ,  C12N9/96 ,  Y10S435/814 ,  Y10S435/816 ,  Y10S435/827 ,  Y10S435/853 ,  Y10S435/886 ,  Y10S435/89

摘要： A process for purifying glucose isomerase comprises the steps of acid treatment and salt fractionation. An enzyme solution is treated with an acid, such as acetic acid, to a pH from about 3.5 to about 5.0. The proteinaceous solids are collected and extracted with a buffer, such as imidazole, whose solution has a pH of about 6 to about 8. The solution is then collected and a salt, such as ammonium sulfate, is dissolved therein from about 40% to about 50% of its saturation point. The proteinaceous solids which form are removed and additional ammonium sulfate is dissolved to attain from about 41% to about 60% of its saturation point, followed by collection of the solids containing purified enzyme. A composition which preserves enzyme activity upon storage of glucose isomerase and which imparts resistance to thermal deactivation of said enzyme comprises an aqueous solution of glycerol, a buffer whose solution is at a pH of about 6 to about 8, divalent cobalt ions and magnesium ions.

摘要翻译： 葡萄糖异构酶的纯化方法包括酸处理和盐分离步骤。 用酸（如乙酸）处理酶溶液至约3.5至约5.0的pH。 收集蛋白质固体并用缓冲液如咪唑提取其溶液，其pH为约6至约8.然后收集溶液，并将盐如硫酸铵溶解在其中约40％至约 其饱和点的50％。 除去形成的蛋白质固体并加入另外的硫酸铵以达到其饱和点的约41％至约60％，然后收集含有纯化酶的固体。 保存葡萄糖异构酶并赋予对所述酶的热失活抗性的酶活性的组合物包含甘油水溶液，其溶液的pH为约6至约8的缓冲液，二价钴离子和镁离子。

公开(公告)号：US4532208A

公开(公告)日：1985-07-30

申请号：US491835

申请日：1983-05-05

申请人： Edmund W. Hafner ,  Denise M. Jackson

发明人： Edmund W. Hafner ,  Denise M. Jackson

IPC分类号： C12N9/92 ,  C12P19/24 ,  C12N1/20 ,  C12R1/465

CPC分类号： C12R1/465 ,  C12N9/92 ,  Y10S435/886

摘要： A mutant Streptomyces coelicolor, NRRL 15398, produces glucose isomerase constitutively at a level at least as great as the parent does inductively in common growth media. The isomerase can be effectively used to isomerize glucose to fructose in a continuous process using immobilized enzyme.

摘要翻译： 突变型天蓝色链霉菌NRRL 15398在组成上产生葡萄糖异构酶，其水平至少与母体在普通生长培养基中感应相同。 异构酶可以有效地用于使用固定化酶在连续过程中将葡萄糖异构化成果糖。