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    FRT recombination sites and methods of use
    5.
    发明授权
    FRT recombination sites and methods of use 有权
    FRT重组位点和使用方法

    公开(公告)号：US08318493B2

    公开(公告)日：2012-11-27

    申请号：US12756211

    申请日：2010-04-08

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： C12N15/87 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

    Novel FRT Recombination Sites and Methods of Use
    6.
    发明申请
    Novel FRT Recombination Sites and Methods of Use 有权
    新型FRT重组站点和使用方法

    公开(公告)号：US20100192263A1

    公开(公告)日：2010-07-29

    申请号：US12756211

    申请日：2010-04-08

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/10 ,  C12N5/04

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。

    FRT recombination sites and methods of use
    7.
    发明授权
    FRT recombination sites and methods of use 有权
    FRT重组位点和使用方法

    公开(公告)号：US07736897B2

    公开(公告)日：2010-06-15

    申请号：US11487300

    申请日：2006-07-14

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： C12N15/87 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。