公开(公告)号：US07749388B2

公开(公告)日：2010-07-06

申请号：US10209508

申请日：2002-07-30

申请人： Derek S. Pai ,  Steven T. Kunitake ,  Derrick Richardson ,  Jorge Monteon

发明人： Derek S. Pai ,  Steven T. Kunitake ,  Derrick Richardson ,  Jorge Monteon

IPC分类号： C07H21/00 ,  B01D15/08 ,  C02F1/28

CPC分类号： B01L3/5021 ,  B01L3/5082 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/046 ,  B01L2300/048 ,  B01L2300/0681 ,  G01N1/40 ,  G01N1/405

摘要： This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.

摘要翻译： 这涉及用于分离核酸的过滤柱，特别是在小洗脱体积下。 过滤柱适用于稳定放置在各种尺寸的标准塑料收集管的上部。 过滤柱的主体具有多个表面，以适应放置在不同尺寸的收集管内。 过滤柱包含核酸特异性过滤器，其可以位于过滤器柱内的交替区域，使用相同的柱体提供不同的过滤器表面积和加载体积容量。 过滤柱在上端具有适于被帽密封的开口。 还提供了使用这种过滤柱回收核酸的方法。

公开(公告)号：US07556733B2

公开(公告)日：2009-07-07

申请号：US11076272

申请日：2005-03-09

申请人： Thomas L. Smith ,  Derek S. Pai ,  Steven T. Kunitake ,  Derrick Richardson ,  Jorge Monteon

发明人： Thomas L. Smith ,  Derek S. Pai ,  Steven T. Kunitake ,  Derrick Richardson ,  Jorge Monteon

IPC分类号： B01D29/00

CPC分类号： G01N1/40 ,  B01L3/5021 ,  B01L3/5082 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/046 ,  B01L2300/048 ,  B01L2300/0681 ,  G01N1/405

摘要： This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.

摘要翻译： 这涉及用于分离核酸的过滤柱，特别是在小洗脱体积下。 过滤柱适用于稳定放置在各种尺寸的标准塑料收集管的上部。 过滤柱的主体具有多个表面，以适应放置在不同尺寸的收集管内。 过滤柱包含核酸特异性过滤器，其可以位于过滤器柱内的交替区域，使用相同的柱体提供不同的过滤器表面积和加载体积容量。 过滤柱在上端具有适于被帽密封的开口。 还提供了使用这种过滤柱回收核酸的方法。

公开(公告)号：US20120329097A1

公开(公告)日：2012-12-27

申请号：US13479001

申请日：2012-05-23

申请人： Mark G. ERLANDER ,  Ranelle Salunga ,  Theresa Taylor ,  Liang You ,  Steven T. Kunitake

发明人： Mark G. ERLANDER ,  Ranelle Salunga ,  Theresa Taylor ,  Liang You ,  Steven T. Kunitake

IPC分类号： C12P19/34

CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2525/179 ,  C12Q2525/143 ,  C12Q2521/107

摘要： The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

摘要翻译： 本发明提供了用于扩增核酸分子的改进方法。 提供了使用寡核苷酸，DNA和RNA聚合酶扩增靶多核苷酸（包括mRNA）的方法。 本发明还提供了用于实施该方法的组合物和试剂盒，以及使用扩增产物的方法。

公开(公告)号：US06238874B1

公开(公告)日：2001-05-29

申请号：US09363247

申请日：1999-07-28

申请人： Kurt R. Jarnagin ,  Steven T. Kunitake ,  Bala S. Manian

发明人： Kurt R. Jarnagin ,  Steven T. Kunitake ,  Bala S. Manian

IPC分类号： G01N33533

CPC分类号： C12M41/46 ,  Y10S436/809

摘要： An apparatus and method of use for assaying cellular motility in response to a concentration gradient of a chemotactic agent. Generally, the apparatus includes a chamber having a region for receiving a biological sample containing cells of interest and, spaced apart from such region, another region for receiving a chemotactic agent. Between these regions, a concentration gradient of chemotactic agent is established. The apparatus further includes an optical system for detecting and mapping the positions of individual cells responsive to such concentration gradient. Means for processing and analyzing the collected data are also provided. Motility determinations may be made on purified or unpurified samples. Single-site assay devices and multi-site, high-throughput assay devices are disclosed.

摘要翻译： 用于响应于趋化剂的浓度梯度来测定细胞运动性的装置和方法。 通常，该装置包括具有用于接收含有感兴趣的细胞的生物样品并与该区域间隔的区域的腔室，用于接收趋化剂的另一区域。 在这些区域之间，建立了趋化剂的浓度梯度。 该装置还包括用于响应于这种浓度梯度来检测和映射各个单元的位置的光学系统。 还提供了处理和分析收集的数据的手段。 可以对纯化或未纯化的样品进行运动测定。 公开了单位点测定装置和多位点，高通量测定装置。

公开(公告)号：US06528248B2

公开(公告)日：2003-03-04

申请号：US09562495

申请日：2000-05-01

申请人： Ann Bennett Lossing ,  Sherrie L. Ransom ,  Steven T. Kunitake ,  Robert H. Reamey ,  David F. Head ,  Hala Al-Shawany

发明人： Ann Bennett Lossing ,  Sherrie L. Ransom ,  Steven T. Kunitake ,  Robert H. Reamey ,  David F. Head ,  Hala Al-Shawany

IPC分类号： A01G506

CPC分类号： G01N1/2813 ,  G01N2001/284

摘要： Systems and methods are described for barriers on laser capture microdissection samples. A method of processing a biological sample for laser capture microdissection, includes: providing the biological sample; and applying a substance to the biological sample so as to provide a barrier between the biological sample and a surrounding environment. The systems and methods provide advantages because non-specific pick-up is reduced, visualization is improved, sample degradation is reduced, and contamination is reduced.

摘要翻译： 描述了用于激光捕获显微解剖样品的障碍的系统和方法。 一种处理用于激光捕获显微切割的生物样品的方法，包括：提供生物样品; 以及将物质施加到所述生物样品以便在所述生物样品和周围环境之间提供屏障。 这些系统和方法提供了优点，因为非特异性吸收降低，可视化得到改善，样品降解降低，污染减少。