公开(公告)号：US20190194356A1

公开(公告)日：2019-06-27

申请号：US16308811

申请日：2017-06-08

申请人： CHANGZHOU BOJIA BIOTECHNOLOGY CO., LTD

发明人： Shaoxiong Wang

IPC分类号： C07K16/40 ,  A61P3/06 ,  G01N33/58 ,  C12N15/62

CPC分类号： C07K16/40 ,  A61K38/00 ,  A61K39/395 ,  A61P3/06 ,  C07K2317/32 ,  C07K2317/565 ,  C07K2319/30 ,  C12N9/64 ,  C12N15/62 ,  G01N33/582 ,  G01N33/583

摘要： Provided is a long-acting PCSK9-specific binding protein and application thereof. Provided is an MV072 protein with unique complementarity-determining regions, i.e., a binding protein specifically binding to proprotein convertase subtilisin kexin type 9 (PCSK9). The protein can specifically bind to PCSK9, effectively inhibit the function of PCSK9 and reduce plasma LDL cholesterol level. Further provided is an application of the binding protein in treating diseases related to or influenced by the function of PCSK9.

公开(公告)号：US09874566B2

公开(公告)日：2018-01-23

申请号：US14818980

申请日：2015-08-05

申请人： University of Virginia Patent Foundation

发明人： Kenneth Brayman ,  Daniel Bowers ,  Cassandra L. Fraser ,  Edward A. Botchwey, III

IPC分类号： A61K8/00 ,  G01N33/58 ,  A61K49/00 ,  G01N33/84

CPC分类号： G01N33/582 ,  A61K49/0015 ,  A61K49/0021 ,  A61K49/0054 ,  A61K49/0095 ,  G01N33/583 ,  G01N33/84 ,  G01N2800/7038

摘要： To address the need for scaffold-based oxygen concentration monitoring, a single-component, self-referenced oxygen sensor was made into nanofibers. Electrospinning process parameters were tuned to produce a biomaterial scaffold with specific morphological features. The ratio of an oxygen sensitive phosphorescence signal to an oxygen insensitive fluorescence signal was calculated at each image pixel to determine an oxygenation value. A single component boron dye-polymer conjugate was chosen for additional investigation due to improved resistance to degradation in aqueous media compared to a dye polymer blend. Standardization curves show that in fully supplemented media, the fibers are responsive to dissolved oxygen concentrations less than 15 parts per million. Spatial and temporal ratiometric gradients were observed in vitro radiating outward from the center of a dense adherent cell grouping. Sensor activation in ischemia and cell transplant models in vivo show oxygenation decreases on the scale of minutes.

公开(公告)号：US09797908B2

公开(公告)日：2017-10-24

申请号：US14338113

申请日：2014-07-22

申请人： The Scripps Research Institute

发明人： Alexander Deiters ,  T. Ashton Cropp ,  Jason W. Chin ,  J. Christopher Anderson ,  Peter G. Schultz

IPC分类号： C12P21/00 ,  G01N33/58 ,  C07C227/22 ,  C07C229/36 ,  C07C269/06 ,  C07C311/41 ,  C07K1/107 ,  C07K1/13 ,  C07K14/00 ,  C09B11/08 ,  C09B57/00 ,  C12N9/00 ,  C12N15/67 ,  C12P17/04 ,  C12P21/02 ,  G01N33/532 ,  C07K16/18

CPC分类号： G01N33/583 ,  C07C227/22 ,  C07C229/36 ,  C07C269/06 ,  C07C311/41 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/001 ,  C07K16/18 ,  C09B11/08 ,  C09B57/00 ,  C12N9/93 ,  C12N15/67 ,  C12N2510/02 ,  C12P17/04 ,  C12P21/02 ,  G01N33/532 ,  G01N33/582 ,  C07C271/22

摘要： This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

公开(公告)号：US09759657B2

公开(公告)日：2017-09-12

申请号：US14576156

申请日：2014-12-18

申请人： Palo Alto Research Center Incorporated

发明人： Peter Kiesel ,  Joerg Martini ,  Michael I. Recht

IPC分类号： G01N21/64 ,  G01N33/542 ,  B01J19/00 ,  G01N33/543 ,  G01N33/58

CPC分类号： G01N21/6428 ,  B01J19/0046 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00587 ,  B01J2219/00596 ,  G01N21/6452 ,  G01N33/542 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/583 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441

摘要： Analysis of a system and/or sample involves the use of absorption-encoded micro beads. Each type of micro bead is encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads. A system and/or a sample can be analyzed using information obtained from detecting the one or more types of absorption-encoded micro beads.

公开(公告)号：US09719986B2

公开(公告)日：2017-08-01

申请号：US14312341

申请日：2014-06-23

申请人： Ventana Medical Systems, Inc.

发明人： Jerome W. Kosmeder, II ,  Mark Lefever ,  Donald Johnson ,  Michael Farrell ,  Zhanna Zhilina ,  Christopher Bieniarz

IPC分类号： G01N33/53 ,  C07D495/04 ,  G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  C12Q1/68

CPC分类号： G01N33/5308 ,  C07D495/04 ,  C12Q1/6804 ,  C12Q1/6841 ,  C12Q1/6886 ,  G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/582 ,  G01N33/583 ,  Y10S435/961 ,  Y10S530/807

摘要： A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

公开(公告)号：US09645140B2

公开(公告)日：2017-05-09

申请号：US14330727

申请日：2014-07-14

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Brian Agnew ,  Kyle Gee ,  Tamara Nyberg

IPC分类号： G01N33/533 ,  G01N33/532 ,  G01N33/58 ,  C12P21/00 ,  A61K47/48 ,  G01N33/531 ,  G01N33/50 ,  C07K16/00 ,  G01N33/534

CPC分类号： C12P21/005 ,  A61K47/549 ,  A61K47/61 ,  C07K1/13 ,  C07K14/47 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C12Y302/01096 ,  G01N33/5005 ,  G01N33/5008 ,  G01N33/531 ,  G01N33/532 ,  G01N33/533 ,  G01N33/534 ,  G01N33/581 ,  G01N33/582 ,  G01N33/583 ,  Y10T436/17

摘要： Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.

公开(公告)号：US20170082615A1

公开(公告)日：2017-03-23

申请号：US15269301

申请日：2016-09-19

申请人： XiuJun Li ,  Guanglei Fu

发明人： XiuJun Li ,  Guanglei Fu

IPC分类号： G01N33/543 ,  G01N25/48 ,  G01N33/553

CPC分类号： G01N25/4813 ,  G01N33/58 ,  G01N33/583 ,  G01N33/587

摘要： Certain embodiments of the invention are directed to a photothermal immunoassay employing a thermometer or colorimetric detection method for sensitive quantitative readout based on the photothermal effect provided by a detection reagent.

公开(公告)号：US20160238611A1

公开(公告)日：2016-08-18

申请号：US15026734

申请日：2014-10-02

申请人： Deutsches Krebsforschungszentrum ,  Ruprecht-Karls-Universität Heidelberg

发明人： Roland EILS ,  Barbara DI VENTURA ,  Lorenz ADLUNG ,  Katharina GENREITH ,  Tim HEINEMANN ,  Hannah MEYER ,  Dominik NIOPEK ,  Fanny GEORGI ,  Ralf BEER ,  Tania Christina CHRISTIANSEN ,  Konrad HERBST ,  Nikolaos IGNATIADIS ,  Ilia KATS ,  Nils KURZAWA ,  Johanna MEICHSNER ,  Sophie RABE ,  Anja RIEDEL ,  Joshua SACHS ,  Julia Patricia SCHESSNER ,  Florian SCHMIDT ,  Philipp Darius Konstantin WALCH

IPC分类号： G01N33/58 ,  C12Q1/02 ,  C12N9/16 ,  C12P21/00 ,  C07K2/00 ,  C12N9/00

CPC分类号： G01N33/583 ,  C07K2/00 ,  C12N9/16 ,  C12N9/93 ,  C12P21/00 ,  C12Q1/02 ,  C12Y301/00 ,  C12Y603/02 ,  G01N2440/00 ,  G01N2458/00

摘要： The present invention relates to a polypeptide or polypeptide complex, comprising at least one non-ribosomal peptide synthesis (NRPS) amino acid module functionally connected to at least one pigment module. The present invention further relates to a labeled oligopeptide comprising a non-naturally attached NRPS pigment or/and polyketide pigment, to a polynucleotide encoding a fusion polypeptide, a vector, preferably an expression vector, comprising the polynucleotide of the present invention and to a host cell comprising the polypeptide or polypeptide complex and/or the polynucleotide, and/or the vector according to the present invention. Moreover, the present invention relates to in vitro and in vivo method of producing a labeled oligopeptide, as well as to methods of optimizing the same.

摘要翻译： 本发明涉及多肽或多肽复合物，其包含与至少一种颜料模块功能连接的至少一种非核糖体肽合成（NRPS）氨基酸模块。 本发明还涉及包含非天然连接的NRPS颜料或/和聚酮化合物颜料的标记的寡肽与编码融合多肽的多核苷酸，载体，优选包含本发明的多核苷酸的载体和宿主 包含多肽或多肽复合物和/或多核苷酸的细胞，和/或根据本发明的载体。 此外，本发明涉及产生标记寡肽的体外和体内方法，以及优化其的方法。

公开(公告)号：US09376706B2

公开(公告)日：2016-06-28

申请号：US14553182

申请日：2014-11-25

申请人： Mologic Limited

发明人： James A. Schouten

IPC分类号： C12Q1/37 ,  B01L3/00 ,  C07K5/083 ,  C07K7/06 ,  G01N33/58

CPC分类号： C12Q1/37 ,  B01L3/5055 ,  B01L2300/0809 ,  C07K5/0808 ,  C07K7/06 ,  G01N33/583

摘要： A polypeptide comprising a chromogenic amino acid. The chromogenic amino acid is flanked by at least one amino acid to the N and C termini thereof. The amine group of the chromogenic amino acid has a pKa of less than 5. The chromogenic amino acid is capable of reacting with a conjugated aldehyde. The polypeptide comprises a target sequence for a target protease which is capable of cleaving the peptide bond comprising the amino group of the chromogenic amino acid.

公开(公告)号：US20150293110A1

公开(公告)日：2015-10-15

申请号：US14683693

申请日：2015-04-10

申请人： SurModics IVD, Inc.

发明人： Sean Lundquist

IPC分类号： G01N33/58

CPC分类号： G01N33/583 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q2326/10 ,  G01N33/581

摘要： The disclosure is directed to compositions, kits, and methods for performing colorimetric analysis. A composition for a colorimetric assay can be prepared that includes an aromatic amine chromogenic substrate and a zwitterionic solubilization reagent that is N,N,N-trimethylglycine (betaine). The zwitterionic solubilization reagent is highly useful as a non-encapsulating solubilization reagent and provided faster kinetics and increased dye content of an aromatic amine chromogenic substrate in an enzymatic reaction. In turn, this can result in improvements in detection of an analyte in a sample and also can increase the sample throughput. The aromatic amine chromogenic substrate and N,N,N-trimethylglycine solubilization reagent can be used in assays such as ELISAs in order to provide a more accurate and faster detection of analytes in a biological sample.

摘要翻译： 本公开涉及用于进行比色分析的组合物，试剂盒和方法。 可以制备用于比色测定的组合物，其包括芳族胺显色底物和N，N，N-三甲基甘氨酸（甜菜碱）的两性离子溶解试剂。 两性离子溶解试剂作为非包封的增溶剂是非常有用的，并提供酶反应中芳族胺显色底物的更快的动力学和增加的染料含量。 反过来，这可以导致样品中分析物的检测的改进，并且还可以增加样品通量。 芳族胺显色底物和N，N，N-三甲基甘氨酸增溶剂可用于诸如ELISA的测定中，以便提供对生物样品中分析物的更准确和更快速的检测。