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    Process for the isolation of compound scopoletin useful as nitric oxide synthesis inhibitor
    1.
    发明授权
    Process for the isolation of compound scopoletin useful as nitric oxide synthesis inhibitor 失效
    用于分离化合物scopoletin的方法可用作一氧化氮合成抑制剂

    公开(公告)号：US06337095B1

    公开(公告)日：2002-01-08

    申请号：US09538893

    申请日：2000-03-30

    申请人： Dharam Chand Jain ,  Neerja Pant ,  Madan Mohan Gupta ,  Rajendra Singh Bhakuni ,  Ram Kishor Verma ,  Sudeep Tandon ,  Shiv Kumar Gupta ,  Amit Tewari ,  Atul Prakash Kahol ,  Sushil Kumar

    发明人： Dharam Chand Jain ,  Neerja Pant ,  Madan Mohan Gupta ,  Rajendra Singh Bhakuni ,  Ram Kishor Verma ,  Sudeep Tandon ,  Shiv Kumar Gupta ,  Amit Tewari ,  Atul Prakash Kahol ,  Sushil Kumar

    IPC分类号： A01N6500

    CPC分类号： C07D311/16

    摘要： The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from Artemisia annua and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

    摘要翻译： 本发明涉及一种用于分离化合物番茄酱的方法，其用作来自青蒿和其他植物家族的一氧化氮合成抑制剂，所述方法包括用比例为1的丙酮酸溶剂萃取不同植物部分的干燥粉末材料 5℃6〜8小时，真空浓缩提取溶剂至30％原始萃取液，用卤代溶剂分配浓缩萃取液，在非极性卤代溶剂中转移香料，用无水硫酸钠干燥卤代溶剂， 蒸发溶剂，结晶甲醇残余物并过滤晶体，浓缩滤液并在硅胶上进行色谱分离，用氯仿甲醇混合物洗脱; 并将含有scopoletin的级分结晶，得到纯的scopoletin化合物。

    Process for the preparation of a extract rich in bacosides from the herb Bacopa monniera
    6.
    发明授权
    Process for the preparation of a extract rich in bacosides from the herb Bacopa monniera 失效
    从草药Bacopa monniera制备富含芽孢杆菌的提取物的方法

    公开(公告)号：US06833143B1

    公开(公告)日：2004-12-21

    申请号：US10400192

    申请日：2003-03-26

    申请人： Atul Prakash Kahol ,  Tarun Singh ,  Sudeep Tandon ,  Madan Mohan Gupta ,  Suman Preet Singh Khanuja

    发明人： Atul Prakash Kahol ,  Tarun Singh ,  Sudeep Tandon ,  Madan Mohan Gupta ,  Suman Preet Singh Khanuja

    IPC分类号： A61K3578

    CPC分类号： A61K36/80

    摘要： The present invention provides a novel process for the preparation of bacosides enriched fraction in a non-hygroscopic form the extract of Bacopa monniera, the said process comprising the steps of drying freshly harvested herb in a hot air oven at 37-42° C., powdering and sieving the dried herb to obtain powder of 30-40 mesh size, defatting the powdered herb with hexane in a modified soxhlet extractor, extracting the defatted powdered herb with acetone, again extracting the same herb with methanol to obtain an extract containing bacosides, concentrating the extract to one twentieth of its original volume under reduced pressure, gradually adding the concentrated extract to acetone for precipitating the bacosides, filtering the bacosides in a Nutsche type vacuum filter, dissolving the crude bacoside mass into 2-10 parts water, extracting the bacoside solution with n-butanol to selectively transfer the bacosides to the solvent phase, separating and concentrating the solvent phase under vacuum to obtain semi-dry mass, dissolving the semi dried mass into 2-10 parts water, adding and stirring 1-5% of &bgr;-cyclodextrin to stabilize the bacosides, spray drying the stabilized bacoside solution by maintaining hot air temperature at 90-110° C., to obtain a stable free flowing fraction of Bacopa monniera rich in bacosides.

    摘要翻译： 本发明提供了一种用于制备以非吸湿形式的Bacopa monniera提取物富集杆菌的部分的新方法，所述方法包括以下步骤：在37-42℃的热空气烘箱中干燥新鲜收获的草本植物， 粉碎和筛分干草，得到30-40目尺寸的粉末，用改性索氏提取器用己烷脱脂粉末，用丙酮提取脱脂粉末草药，再次用甲醇提取相同的草本，得到含有药剂的提取物， 将提取物在减压下浓缩至其原始体积的二十分之一，逐渐将浓缩提取物加入到丙酮中沉淀芽孢杆菌，过滤Nutsche型真空过滤器中的芽孢杆菌，将粗制药物块溶解在2-10份水中， 使用正丁醇选择性转移杆菌苷至溶剂相，分离和浓缩溶剂相 获得半干质量，将半干质量溶解在2-10份水中，加入并搅拌1-5％的β-环糊精以稳定芽孢杆菌，通过将热空气温度保持在90℃喷雾干燥稳定的bacoside溶液， 110℃，以获得富含芽孢杆菌的Bacopa monniera的稳定的自由流动分数。

    Process for isolation of hepatoprotective agent silymarin from the seeds of the plant Silybum marianum
    7.
    发明授权
    Process for isolation of hepatoprotective agent silymarin from the seeds of the plant Silybum marianum 失效
    从植物水飞蓟马种子中分离肝脏保护剂水飞蓟素的方法

    公开(公告)号：US06309678B1

    公开(公告)日：2001-10-30

    申请号：US09541035

    申请日：2000-03-31

    申请人： Atul Prakash Kahol ,  Kiran Lata Singh ,  Sudeep Tandon ,  Sushil Kumar

    发明人： Atul Prakash Kahol ,  Kiran Lata Singh ,  Sudeep Tandon ,  Sushil Kumar

    IPC分类号： A01N6500

    CPC分类号： C07D311/20 ,  A61K36/28

    摘要： The invention relates to a novel process for the isolation of a hepatoprotective agent Silymarin from the seeds of the plant Silybum marianum comprising (i) Precooling the seeds to (−) 20° C. for 24 hours in a deep freezer/cold room. (ii) Powdering the cooled seeds in a hammer mill, fitted with about 40 mesh discharge screen. (iii) Defatting the seeds by extracting with hexane in a soxhlet type extractor to remove the total quantity of fatty oil without using a scrain. (iv) Extracting the defatted seeds with acetonitrile at 20-30° C. to extract silymarin fraction. (v) Concentration of the sensitive silymarin fraction under vacuum in a agitated wiped thin film evaporator (vi) stirring the silymarin such dry powder with cold dichloromethane at 5° C. followed by filtration and drying with a slow purge of nitrogen gas. (vii) Further purification of silymarin by suspending in 5 times its weight of acetonitrile and precipitating by 8-12 times its weight of water at 20° C. (viii) Filtering the precipitated silymarin in a closed vacuum filter and having 1-2 &mgr;m screen washing the cake three times with distilled water (ix) Drying of Silymarin cake in vacuum oven to obtain substantially pure silymarin.

    摘要翻译： 本发明涉及一种用于从植物水飞蓟种子种子中分离肝保护剂水飞蓟素的新方法，其包括（i）在深冷冻室/冷室中将种子预冷至（ - ）20℃24小时。 （ii）将冷却的种子粉碎在装有约40目排放筛网的锤磨机中。 （iii）用索氏提取器中的己烷萃取除去种子以除去脂肪油的总量，而不用刮擦。 （iv）在20-30℃用乙腈提取脱脂种子以提取水飞蓟素级分。 （v）在搅拌的擦拭薄膜蒸发器中真空浓缩敏感的水飞蓟素馏分（vi）在5℃下用冷二氯甲烷搅拌水飞蓟素干燥粉末，然后过滤并用氮气的缓慢吹扫干燥。 （vii）通过悬浮5倍重量乙腈并在20℃下沉淀8-12倍重量的水进一步纯化水飞蓟素。（viii）将沉淀的水飞蓟素在封闭的真空过滤器中过滤并具有1-2mum 用蒸馏水洗涤蛋糕三次（ix）在真空烘箱中干燥水飞蓟饼以获得基本上纯的水飞蓟素。

    Bio-reactor for enhancing the biomass yield of plant organs
    10.
    发明授权
    Bio-reactor for enhancing the biomass yield of plant organs 失效
    用于提高植物器官生物量产量的生物反应器

    公开(公告)号：US06589780B2

    公开(公告)日：2003-07-08

    申请号：US09736434

    申请日：2000-12-15

    申请人： Suchitra Banerjee ,  Arun Kumar Kukreja ,  Praveen Chandra Verma ,  Atul Prakash Kahol ,  Sushil Kumar

    发明人： Suchitra Banerjee ,  Arun Kumar Kukreja ,  Praveen Chandra Verma ,  Atul Prakash Kahol ,  Sushil Kumar

    IPC分类号： C12N500

    CPC分类号： A01H4/001 ,  C12M27/02 ,  C12M27/20 ,  C12M27/22 ,  C12M29/06

    摘要： A bio-reactor for enhancing the biomass yield using a closed sterile vessel having a medium, said reactor comprising a central shaft, a support matrix oriented horizontally and dividing the vessel into an upper and a lower chamber, a means for stirring selected from turbine-2,4,6-impeller; marine blade impeller; helical blade impeller; mounted onto the central shaft and located at a predetermined distance from the support matrix so as to generate both radially and axially directed volumetric flow of the media; and at least two semi-circular spargers located both in the upper and lower compartments of the vessel at a predetermined distance from the support matrix.

    摘要翻译： 一种用于使用具有介质的封闭无菌容器提高生物质产量的生物反应器，所述反应器包括中心轴，水平取向并将容器分成上下室的支撑体，用于搅拌的装置， 2,4,6叶轮 船用叶片叶轮; 螺旋叶片叶轮; 安装在中心轴上并且位于与支撑矩阵相距预定距离处，以便产生介质的径向和轴向定向的体积流动; 以及位于容器的上部和下部隔室中的至少两个半圆形喷射器，距离支撑矩阵预定距离。