公开(公告)号：US07189354B2

公开(公告)日：2007-03-13

申请号：US10221527

申请日：2001-03-20

申请人： Dianna Innerst ,  Adonis Kassinos ,  C. Benjamin Wooley ,  Brett Wilmarth ,  William J. Sell ,  Emi Zychlinsky ,  Donald Marino ,  Sandy Yamada ,  Renee Ryan ,  Karen Ding ,  Michael Zatzke

发明人： Dianna Innerst ,  Adonis Kassinos ,  C. Benjamin Wooley ,  Brett Wilmarth ,  William J. Sell ,  Emi Zychlinsky ,  Donald Marino ,  Sandy Yamada ,  Renee Ryan ,  Karen Ding ,  Michael Zatzke

IPC分类号： G01N21/00 ,  G01N31/22 ,  G01N15/06 ,  G01N33/00 ,  C12Q1/68

CPC分类号： B01L3/5025 ,  B01L2300/087 ,  B01L2400/0406 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/76 ,  G01N2021/6482 ,  Y10T436/11

摘要： An enclosed chamber is used in a system for screening of a liquid specimen through binding assays. The enclosed chamber includes an inlet, an outlet, and a plurality of discrete reactant containing wells communicated by a common reagent flow path between the inlet and the outlet. A transparent member or coverslip defines on an inside thereof the plurality of wells. Each well has a bottom for receiving an allergent/antigen/reactant, which emits light upon reacting. The coverslip can optionally define at least one lens at each well. A bottom encloses the plurality of wells and defines between the inlet and the outlet a common reagent flow path between the inlet and the outlet. This bottom defines for each of the plurality of wells a flow-diverting member. An opaque partition is disclosed for surrounding the individual lenses and generally isolating the light path from each well.

摘要翻译： 在通过结合测定筛选液体样品的系统中使用封闭室。 封闭室包括入口，出口和多个离散的反应物容纳孔，其通过入口和出口之间的公共试剂流路连通。 透明构件或盖玻片在其内部限定多个孔。 每个井都有一个底部，用于接收过敏原/抗原/反应物，反应会发光。 盖玻片可以可选地在每个孔处限定至少一个透镜。 底部包围多个孔并且在入口和出口之间限定入口和出口之间的公共试剂流动路径。 该底部为多个井中的每一个限定了一个流动分流构件。 公开了一种不透明分隔件，用于围绕各个透镜，并且通常将光路与每个孔隔离。