公开(公告)号：US06391657B1

公开(公告)日：2002-05-21

申请号：US08598264

申请日：1996-02-07

申请人： Dieter Bernhardt ,  Albrecht Gröner ,  Thomas Nowak

发明人： Dieter Bernhardt ,  Albrecht Gröner ,  Thomas Nowak

IPC分类号： G01N33537

CPC分类号： C07K1/34

摘要： The invention relates to the removal of viruses from aqueous solutions, as a rule protein solutions, by ultrafiltration. This entails the viruses to be removed being increased in size by incubation with a high molecular weight receptor binding thereto, preferably a specific antibody, so that, on the one hand, the separation effect is improved and, on the other hand, a larger pore diameter which can now be chosen for the filters used also makes it possible for smaller viruses to be separated from larger protein molecules present in protein solutions, and, where appropriate, the filtration rate is increased.

摘要翻译： 本发明涉及通过超滤作为规则的蛋白质溶液从水溶液中除去病毒。 这导致通过与结合其的高分子量受体（优选特异性抗体）一起孵育来除去大小的病毒，从而一方面提高了分离效果，另一方面，较大的孔 现在可以选择用于所用过滤器的直径使得可以将较小的病毒与存在于蛋白质溶液中的较大的蛋白质分子分离，并且在适当的情况下，过滤速率增加。

公开(公告)号：US06399356B1

公开(公告)日：2002-06-04

申请号：US09364050

申请日：1999-07-30

申请人： Dieter Bernhardt ,  Albrecht Gröner

发明人： Dieter Bernhardt ,  Albrecht Gröner

IPC分类号： C12N706

CPC分类号： A01N31/14 ,  A01N31/06 ,  A01N31/08 ,  A01N31/16

摘要： A process and an agent for the sanitization of tissues or cell cultures and also of materials and equipment which can be contaminated with viruses are described. For sanitization, a solution of a substituted phenol or of a substituted phenol ether is employed here which as substituents carries one or two saturated or unsaturated hydrocarbon radicals which in each case can have up to 4 carbon atoms.

摘要翻译： 描述了用于组织或细胞培养物的消毒过程以及可被病毒污染的材料和设备的方法和试剂。 为了消毒，在此使用取代的苯酚或取代的苯酚醚的溶液，其作为取代基带有一个或两个饱和或不饱和的烃基，其在每种情况下可具有至多4个碳原子。

公开(公告)号：US06242239B1

公开(公告)日：2001-06-05

申请号：US09336675

申请日：1999-06-21

申请人： Albrecht Gröner ,  Jürgen Römisch

发明人： Albrecht Gröner ,  Jürgen Römisch

IPC分类号： C07K1481

CPC分类号： C12N7/00 ,  A61L2/0017 ,  A61L2/0082 ,  C12N2740/16051

摘要： A process for separating HIV from a fluid is described, in which the HIV is bound to a C1 esterase inhibitor immobilized on a support material. The process can be carried out both for the preparation of HIV-free blood donations and therapeutically for the reduction of the virus load in the blood by means of a blood lavage under the conditions of an extracorporeal blood circulation. The C1 esterase inhibitor can be bonded to a support material which is customary in affinity chromatography or to the fibers of a filter.

摘要翻译： 描述了从流体中分离HIV的方法，其中HIV与固定在载体材料上的C1酯酶抑制剂结合。 该过程可以用于制备无HIV血液捐赠，并且在体外血液循环的条件下通过血液灌洗在治疗上用于减少血液中的病毒载量。 C1酯酶抑制剂可以结合到通常在亲和层析中或过滤器的纤维上的载体材料。

公开(公告)号：US06656720B2

公开(公告)日：2003-12-02

申请号：US10194784

申请日：2002-07-12

申请人： Albrecht Gröner ,  Jürgen Vorlop

发明人： Albrecht Gröner ,  Jürgen Vorlop

IPC分类号： C12N700

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5252 ,  C12N2760/16134 ,  C12N2760/16151

摘要： Animal cells are described which can be infected by influenza viruses and which are adapted to growth in suspension in serum-free medium. Processes for the replication of influenza viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the influenza viruses obtainable by the process or constituents thereof

公开(公告)号：US06455298B1

公开(公告)日：2002-09-24

申请号：US09155581

申请日：1998-09-29

申请人： Albrecht Gröner ,  Jürgen Vorlop

发明人： Albrecht Gröner ,  Jürgen Vorlop

IPC分类号： C12N700

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5252 ,  C12N2760/16134 ,  C12N2760/16151

摘要： Animal cells are described which can be infected by influenza viruses and which are adapted to growth in suspension in serum-free medium. Processes for the replication of influenza viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the influenza viruses obtainable by the process or constituents thereof.

摘要翻译： 描述了可以被流感病毒感染并且适于在无血清培养基中悬浮生长的动物细胞。 此外还描述了使用这些细胞在细胞培养物中复制流感病毒的方法，以及含有通过方法或其组成可获得的流感病毒的疫苗。