公开(公告)号：US20110129879A1

公开(公告)日：2011-06-02

申请号：US12063678

申请日：2007-04-28

申请人： Do Man Kim ,  Mi Young Seo

发明人： Do Man Kim ,  Mi Young Seo

IPC分类号： C12P19/18 ,  C07H21/04 ,  C12N9/10

CPC分类号： C12N9/244 ,  C07K2319/00 ,  C12N9/1051 ,  C12P19/08 ,  C12P19/18 ,  C12Y204/01005 ,  C12Y302/01006

摘要： Disclosed are hybrid genes of glucanase and dextransucrase, recombinant vectors comprising said hybrid genes, microorganisms which are transformed with said recombinant vectors, hybrid enzymes which are expressed from said hybrid genes, and processes for preparing isomalto-oligosaccharides or dextran using said microorganisms or enzymes. Expensive isomalto-oligosaccharides and low molecular weight dextran for clinical use can be produced simply and effectively from cheap substrate-sucrose, using a single bacterial strain or enzyme.

摘要翻译： 公开了葡聚糖酶和葡聚糖蔗糖酶的杂交基因，包含所述杂交基因的重组载体，用所述重组载体转化的微生物，从所述杂交基因表达的杂合酶，以及使用所述微生物或酶制备异麦芽寡聚糖或葡聚糖的方法。 昂贵的异麦芽寡糖和临床使用的低分子量葡聚糖可以使用单一细菌菌株或酶，从廉价的底物蔗糖中简单有效地制备。

公开(公告)号：US08192956B2

公开(公告)日：2012-06-05

申请号：US12063678

申请日：2007-04-28

申请人： Do Man Kim ,  Mi Young Seo

发明人： Do Man Kim ,  Mi Young Seo

IPC分类号： C12P21/04 ,  C12P21/06 ,  C12P19/18 ,  C12N9/10 ,  C12N1/20 ,  C12N15/70 ,  C07H21/04

CPC分类号： C12N9/244 ,  C07K2319/00 ,  C12N9/1051 ,  C12P19/08 ,  C12P19/18 ,  C12Y204/01005 ,  C12Y302/01006

摘要： Disclosed are hybrid genes of glucanase and dextransucrase, recombinant vectors comprising said hybrid genes, microorganisms which are transformed with said recombinant vectors, hybrid enzymes which are expressed from said hybrid genes, and processes for preparing isomalto-oligosaccharides or dextran using said microorganisms or enzymes. Expensive isomalto-oligosaccharides and low molecular weight dextran for clinical use can be produced simply and effectively from cheap substrate-sucrose, using a single bacterial strain or enzyme.

摘要翻译： 公开了葡聚糖酶和葡聚糖蔗糖酶的杂交基因，包含所述杂交基因的重组载体，用所述重组载体转化的微生物，从所述杂交基因表达的杂合酶，以及使用所述微生物或酶制备异麦芽寡聚糖或葡聚糖的方法。 昂贵的异麦芽寡糖和临床使用的低分子量葡聚糖可以使用单一细菌菌株或酶，从廉价的底物蔗糖中简单有效地制备。