公开(公告)号：US08541229B2

公开(公告)日：2013-09-24

申请号：US12688764

申请日：2010-01-15

申请人： Donald L. Court ,  Costantino Nina

发明人： Donald L. Court ,  Costantino Nina

IPC分类号： C12N15/00 ,  C12N15/73

CPC分类号： C12N15/74 ,  C12N7/00 ,  C12N15/70 ,  C12N15/73 ,  C12N2795/10321 ,  C12N2795/10343

摘要： Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.

摘要翻译： 公开了可用于将重组功能引入宿主细胞的λ噬菌体。 还公开了可用于向多种大肠杆菌菌株和其它细菌（包括沙门氏菌，假单胞菌，蓝细菌，螺旋体）赋予重组功能的质粒。 这些质粒和噬菌体可以在体外分离，并可用于转化细菌细胞，如革兰氏阴性细菌。