公开(公告)号：US20070104408A1

公开(公告)日：2007-05-10

申请号：US11379348

申请日：2006-04-19

申请人： Douglas Cyr ,  Roger Farrow ,  Don Arnold

发明人： Douglas Cyr ,  Roger Farrow ,  Don Arnold

IPC分类号： G02B6/00

CPC分类号： G01N30/74 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/161 ,  G01N21/05 ,  G01N30/6086 ,  G01N30/6095 ,  G01N2021/0346 ,  G01N2030/746 ,  Y10T156/1052

摘要： A microfluidic detection device provides reduced dispersion of axial concentration gradients in a flowing sample. The microfluidic detection device includes a cell body and a flow path through the cell body. The flow path has an inlet segment, an outlet segment, and a central segment, which forms a detection cell. The central segment is located between and at an angle with both the inlet segment and the outlet segment. The central segment has a first junction with the inlet segment and a second junction with the outlet segment. The cell body contains two arms that can transmit light to and from the detection cell. At least a portion of a first arm is located in the first junction and at least a portion of a second arm is located in the second junction. The portions of the arms located in the junctions are situated so that fluid entering or exiting the central segment of the flow path flows around the outer surface of one of the portions. By ensuring that the flow velocity is high near the walls both at the beginning and at the end of the conduit, the configuration serves to counteract dispersion caused by the normal parabolic velocity profile of flow through a cylindrical conduit, where the fluid velocity is highest at the center. In addition, the configuration promotes efficient sweeping of the entire volume between the two arms. A method for manufacturing the microfluidic detection device is also provided.

摘要翻译： 微流体检测装置在流动的样品中提供减小的轴向浓度梯度的分散。 微流体检测装置包括细胞体和通过细胞体的流路。 流路具有入口段，出口段和形成检测单元的中心段。 中心段位于入口段和出口段之间并与之成一定角度。 中心段具有与入口段的第一结和与出口段的第二结。 电池体包含两个可以将光传输到检测电池和从检测电池传输光的臂。 第一臂的至少一部分位于第一接合处，并且第二臂的至少一部分位于第二接合处。 位于接合处的臂的部分被定位成使得进入或离开流动路径的中心段的流体围绕其中一个部分的外表面流动。 通过确保在导管的开始和结束处的壁附近的流速高，该构造用于抵消由通过圆柱形导管的流动的正常抛物线速度分布引起的色散，其中流体速度最高在 中心。 此外，该配置促进了在两个臂之间的整个体积的有效扫描。 还提供了一种用于制造微流体检测装置的方法。