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公开(公告)号：US07320876B2
公开(公告)日：2008-01-22
申请号：US10284962
申请日：2002-10-31
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US20110318449A1
公开(公告)日：2011-12-29
申请号：US13154719
申请日：2011-06-07
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US20110086127A1
公开(公告)日：2011-04-14
申请号：US12946821
申请日：2010-11-15
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US07833743B2
公开(公告)日：2010-11-16
申请号：US11963587
申请日：2007-12-21
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US20090074909A1
公开(公告)日：2009-03-19
申请号：US11963587
申请日：2007-12-21
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US07972805B2
公开(公告)日：2011-07-05
申请号：US12946821
申请日：2010-11-15
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US08551724B2
公开(公告)日：2013-10-08
申请号：US13154719
申请日：2011-06-07
申请人： Douglas M. Webel , Donald E. Orr , Frank E. Ruch , Xingen Lei
发明人： Douglas M. Webel , Donald E. Orr , Frank E. Ruch , Xingen Lei
CPC分类号： C12Q1/48 , A23K20/189 , A23K20/26 , A23K50/00 , A23K50/75 , A23K50/80 , C12N9/16 , C12Y301/03008 , Y02A40/818
摘要： A method is described for improving the nutritional value of a foodstuff comprising a source of myo-inositol hexakisphosphate by feeding the foodstuff in combination with a phytase expressed in yeast. The method comprises the step of feeding the animal the foodstuff in combination with a phytase expressed in yeast wherein the phytase can be selected from the group consisting of AppA1, AppA2 and a site-directed mutant of AppA. The invention also enables reduction of the feed to weight gain ratio and an increase bone mass and mineral content of an animal. A foodstuff and a feed additive comprising AppA2 or a site-directed mutant of AppA are also described.
摘要翻译： 描述了一种用于通过将食物与酵母中表达的植酸酶组合进料来提高包含肌醇六磷酸肌源的食品的营养价值的方法。 该方法包括将动物饲料与酵母中表达的植酸酶组合的步骤，其中植酸酶可以选自AppA1，AppA2和AppA的定点突变体。 本发明还能够减少饲料与体重增加比并增加动物的骨质量和矿物质含量。 还描述了包含AppA2或AppA的位点定向突变体的食品和饲料添加剂。
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公开(公告)号：US07736680B2
公开(公告)日：2010-06-15
申请号：US11952467
申请日：2007-12-07
申请人： Xingen Lei , Edward J. Mullaney , Abul Ullah
发明人： Xingen Lei , Edward J. Mullaney , Abul Ullah
CPC分类号： C12Y301/03026 , A23K10/14 , A23K20/189 , A23L5/25 , A23L11/33 , A23L29/06 , C12N9/16 , C12Y301/03008
摘要： The present invention relates to an isolated nucleic acid molecule encoding a mutant phytase and the isolated mutant phytase itself. The present invention further relates to methods of using the isolated nucleic acid molecule and the isolated mutant phytase of the present invention.
摘要翻译： 本发明涉及编码突变体植酸酶的分离的核酸分子和分离的突变体植酸酶本身。 本发明还涉及使用本发明的分离的核酸分子和分离的突变体植酸酶的方法。
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公开(公告)号：US07300781B2
公开(公告)日：2007-11-27
申请号：US11018709
申请日：2004-12-20
申请人： Xingen Lei
发明人： Xingen Lei
CPC分类号： C12Y301/03026 , A23K20/189 , C12N9/16 , C12Y301/03008
摘要： The present invention relates to an isolated mutant acid phosphatase/phytase with improved enzymatic properties. The mutant acid phosphatase/phytase composition is particularly useful in animal feed compositions.
摘要翻译： 本发明涉及具有改善酶特性的分离的突变型酸性磷酸酶/植酸酶。 突变型酸性磷酸酶/植酸酶组合物在动物饲料组合物中特别有用。
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公开(公告)号：US20050095691A1
公开(公告)日：2005-05-05
申请号：US11018709
申请日：2004-12-20
申请人： Xingen Lei
发明人： Xingen Lei
CPC分类号： C12Y301/03026 , A23K20/189 , C12N9/16 , C12Y301/03008
摘要： The present invention relates to an isolated mutant acid phosphatase/phytase with improved enzymatic properties. The mutant acid phosphatase/phytase composition is particularly useful in animal feed compositions.
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