公开(公告)号：US20050079556A1

公开(公告)日：2005-04-14

申请号：US10697737

申请日：2003-10-29

申请人： Douglas Nixon ,  Adrian McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N33/50 ,  G01N33/58 ,  G01N33/567

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。

公开(公告)号：US07186516B2

公开(公告)日：2007-03-06

申请号：US10697737

申请日：2003-10-29

申请人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N33/567 ,  C12Q1/00

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。

公开(公告)号：US06673556B2

公开(公告)日：2004-01-06

申请号：US09954392

申请日：2001-09-12

申请人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

发明人： Douglas Nixon ,  Adrian B. McDermott ,  Scott Furlan ,  Martin Bigos ,  Megan Sheehy ,  Paul Klenerman

IPC分类号： G01N3353

CPC分类号： G01N33/5005 ,  G01N33/582 ,  G01N2500/10 ,  G01N2800/52

摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.

摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。