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公开(公告)号：US07186516B2
公开(公告)日：2007-03-06
申请号：US10697737
申请日：2003-10-29
申请人： Douglas Nixon , Adrian B. McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
发明人： Douglas Nixon , Adrian B. McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
IPC分类号： G01N33/567 , C12Q1/00
CPC分类号： G01N33/5005 , G01N33/582 , G01N2500/10 , G01N2800/52
摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.
摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。
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公开(公告)号：US20050079556A1
公开(公告)日：2005-04-14
申请号：US10697737
申请日：2003-10-29
申请人： Douglas Nixon , Adrian McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
发明人： Douglas Nixon , Adrian McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
IPC分类号： G01N33/50 , G01N33/58 , G01N33/567
CPC分类号： G01N33/5005 , G01N33/582 , G01N2500/10 , G01N2800/52
摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.
摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供了检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。
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公开(公告)号：US20130012429A1
公开(公告)日：2013-01-10
申请号：US13176994
申请日：2011-07-06
CPC分类号： C12N15/113 , A61K31/341 , A61K31/4196 , A61K38/1709 , A61K38/21 , G01N33/5082 , G01N33/5767 , G01N2333/186 , G01N2800/52 , Y02A50/411
摘要： This invention relates to a method of treating viral infections, and compounds for use in the treatment of viral infections by modulating the BMP/SMAD signalling pathway. In particular, it relates to methods and compounds for treating hepatitis C virus infection and/or influenza virus infection. It also relates to methods for identifying compounds that are useful in the treatment of viral infections, in particular hepatitis C virus infection and/or influenza virus infection.
摘要翻译： 本发明涉及一种治疗病毒感染的方法，以及通过调节BMP / SMAD信号通路用于治疗病毒感染的化合物。 特别涉及用于治疗丙型肝炎病毒感染和/或流感病毒感染的方法和化合物。 它还涉及用于鉴定可用于治疗病毒感染，特别是丙型肝炎病毒感染和/或流感病毒感染的化合物的方法。
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公开(公告)号：US06673556B2
公开(公告)日：2004-01-06
申请号：US09954392
申请日：2001-09-12
申请人： Douglas Nixon , Adrian B. McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
发明人： Douglas Nixon , Adrian B. McDermott , Scott Furlan , Martin Bigos , Megan Sheehy , Paul Klenerman
IPC分类号： G01N3353
CPC分类号： G01N33/5005 , G01N33/582 , G01N2500/10 , G01N2800/52
摘要： The present invention provides methods of detecting specific lysis of a cell by a lytic agent. The methods generally involve contacting a labeled target cell with a lytic agent; and detecting fluorescence in the target cell. The target cells are labeled with two fluorescent labels: a first fluorescent label that labels the plasma membrane; and a second fluorescent label that labels the cytosol. Release of the cytosolic label from the target cell indicates that the target cell has been lysed. The invention further provides methods of detecting the presence in a sample of a cell that specifically lyses a target cell. The invention further provides methods of detecting the presence in a sample of an antibody that specifically lyses a target cell. The methods are useful in a variety of applications.
摘要翻译： 本发明提供了通过溶解剂检测细胞的特异性裂解的方法。 所述方法通常包括使标记的靶细胞与裂解剂接触; 并检测靶细胞中的荧光。 靶细胞用两个荧光标记标记：标记质膜的第一个荧光标记; 和标记细胞溶质的第二荧光标记。 从靶细胞释放细胞溶质标记表明目标细胞已经裂解。 本发明还提供了检测特异性裂解靶细胞的细胞样品中存在的方法。 本发明还提供检测特异性裂解靶细胞的抗体样品中存在的方法。 这些方法在各种应用中是有用的。
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公开(公告)号：US06506555B1
公开(公告)日：2003-01-14
申请号：US09719140
申请日：2001-06-19
IPC分类号： C12Q170
CPC分类号： A61K31/427 , A61K31/4725
摘要： The invention relates to the use of at least one compound that inhibits HIV (human immunodeficiency virus) protease, selected from ritonavir, saquinavir or one of the pharmaceutically acceptable salts thereof, in association with a pharmaceutically acceptable vehicle for the production of a medicament to modulate proteasome.
摘要翻译： 本发明涉及使用至少一种抑制HIV（人类免疫缺陷病毒）蛋白酶的化合物，所述化合物选自利托那韦，沙奎那韦或其药学上可接受的盐之一，与药学上可接受的载体相结合，用于制备药物的调节 蛋白酶体
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