公开(公告)号：US20190085344A1

公开(公告)日：2019-03-21

申请号：US16209028

申请日：2018-12-04

申请人： Dow AgroSciences LLC

发明人： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：US20150040267A1

公开(公告)日：2015-02-05

申请号：US14458086

申请日：2014-08-12

申请人： Dow AgroSciences LLC

发明人： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

摘要翻译： 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

公开(公告)号：US10160975B2

公开(公告)日：2018-12-25

申请号：US14458086

申请日：2014-08-12

申请人： Dow AgroSciences LLC

发明人： W. Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.