公开(公告)号：US09896692B2

公开(公告)日：2018-02-20

申请号：US14570823

申请日：2014-12-15

申请人： Dow AgroSciences LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  D. Ry Wagner ,  Xing Liang Liu

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8209 ,  C12N15/8205 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

摘要： An activation tagging construct for maize that includes one or more sugarcane bacilliform viral (SCBV) enhancer elements, and resulting tagged populations and plants, are described. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

公开(公告)号：US20150040267A1

公开(公告)日：2015-02-05

申请号：US14458086

申请日：2014-08-12

申请人： Dow AgroSciences LLC

发明人： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

摘要翻译： 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

公开(公告)号：US09493779B2

公开(公告)日：2016-11-15

申请号：US14019293

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： William Michael Ainley ,  Steven R. Webb ,  Pon Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC分类号： C12N15/82 ,  C12N5/14 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

摘要翻译： FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。

公开(公告)号：US20150067921A1

公开(公告)日：2015-03-05

申请号：US14019211

申请日：2013-09-05

申请人： Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/79 ,  C12N15/82 ,  C12N9/22 ,  C12N15/63

CPC分类号： C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

摘要翻译： 通过以位点方式切割细胞中FAD3基因的位置，在FAD3基因座中进行基因编辑或基因堆积的方法，以在FAD3基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。

公开(公告)号：US09963711B2

公开(公告)日：2018-05-08

申请号：US14019244

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/82 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C07K14/47

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US09914930B2

公开(公告)日：2018-03-13

申请号：US14019211

申请日：2013-09-05

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/82 ,  C12N9/22 ,  C12N15/79 ,  C12N15/63

CPC分类号： C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20150059021A1

公开(公告)日：2015-02-26

申请号：US14381985

申请日：2013-02-28

申请人： DOW AGROSCIENCES LLC

发明人： Patricia Ann Owens Merlo ,  Cory Larsen ,  Scott A. Bevan ,  John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  Mark Allen Thompson

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

摘要翻译： 鉴定新的增强子序列在植物功能基因组学中具有显着的效用。 已经鉴定出甘蔗杆状坏死病毒（SCBV）转录增强子。 该增强子可用于增加基因启动子的转录速率和激活标记实验。 当将4×阵列的SCBV增强子置于玉米醇脱氢酶最小启动子的截短形式的上游时，观察到转录增加10倍。 描述了使用SCBV转录增强子的方法，嵌合转录调控区，构建体，细胞，组织和包含增强子一个或多个拷贝的生物体也是如此。

公开(公告)号：US20140090112A1

公开(公告)日：2014-03-27

申请号：US14019244

申请日：2013-09-05

申请人： Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  William Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

摘要翻译： FAD2基因座中基因编辑或基因堆积的方法，以位点方式，以细胞中的FAD2基因的位置切割，在FAD2基因中产生断裂，然后连接到断裂中，与核酸分子缔合 公开了一种或多种感兴趣的特征。

公开(公告)号：US20190085344A1

公开(公告)日：2019-03-21

申请号：US16209028

申请日：2018-12-04

申请人： Dow AgroSciences LLC

发明人： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：US10077450B2

公开(公告)日：2018-09-18

申请号：US14381985

申请日：2013-02-28

申请人： DOW AGROSCIENCES LLC

发明人： Patricia Ann Owens Merlo ,  Cory Larsen ,  Scott A. Bevan ,  John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  Mark Allen Thompson

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.