公开(公告)号：US20150064708A1

公开(公告)日：2015-03-05

申请号：US14475968

申请日：2014-09-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael AINLEY ,  Jayakumar P. SAMUEL ,  Zehui CAO ,  Liu Y. SHEN ,  Cristie M. DEWES

IPC: C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座上的供体DNA多核苷酸插入物的高通量过程。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

公开(公告)号：US20180010200A1

公开(公告)日：2018-01-11

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi SASTRY-DENT ,  W. Michael AINLEY ,  Jayakumar P. SAMUEL ,  Zehui CAO ,  Liu SHEN ,  Cristie M. DEWES

IPC: C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.