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公开(公告)号：US20150143588A1
公开(公告)日：2015-05-21
申请号：US14531710
申请日：2014-11-03
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi SASTRY-DENT , Steven L. EVANS , Ryan C. BLUE , Zehui CAO
IPC: C12N15/82
CPC classification number: C12N15/8213 , C12N15/8209 , C12N15/8212 , C12N15/8275
Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.
Abstract translation: 描述了可以在植物基因组中的靶位置插入通用供体多核苷酸，以促进供体分子在特定基因组位置内的快速和高通量整合。
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公开(公告)号：US20150128310A1
公开(公告)日：2015-05-07
申请号：US14531739
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.
Abstract translation: 如本文所公开的，已经鉴定了代表用于靶向插入外源序列的最佳位点的来自玉米植物的最佳天然基因座。
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公开(公告)号：US20150064708A1
公开(公告)日：2015-03-05
申请号：US14475968
申请日：2014-09-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , W. Michael AINLEY , Jayakumar P. SAMUEL , Zehui CAO , Liu Y. SHEN , Cristie M. DEWES
IPC: C12Q1/68
CPC classification number: C12Q1/6895
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座上的供体DNA多核苷酸插入物的高通量过程。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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公开(公告)号：US20180010200A1
公开(公告)日：2018-01-11
申请号：US15705447
申请日：2017-09-15
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi SASTRY-DENT , W. Michael AINLEY , Jayakumar P. SAMUEL , Zehui CAO , Liu SHEN , Cristie M. DEWES
IPC: C12Q1/68
CPC classification number: C12Q1/6895
Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.
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公开(公告)号：US20150128309A1
公开(公告)日：2015-05-07
申请号：US14531705
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER , Navin ELANGO
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.
Abstract translation: 如本文所公开的，在单子叶植物（例如玉米植物）中鉴定出最佳天然基因组基因座，其代表用于靶向插入外源序列的最佳位点。
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Patent Agency Ranking
公开(公告)号：US20150128308A1
公开(公告)日：2015-05-07
申请号：US14531748
申请日：2014-11-03
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER , W. Michael AINLEY
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
Abstract translation: 如本文所公开的，已经鉴定出代表用于靶向插入外源序列的最佳位点的大豆植物的最佳天然基因组位点。
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公开(公告)号：US20210230617A1
公开(公告)日：2021-07-29
申请号：US17224659
申请日：2021-04-07
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER , W. Michael AINLEY
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US20190194674A1
公开(公告)日：2019-06-27
申请号：US16263133
申请日：2019-01-31
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER , Navin ELANGO
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US20190136265A1
公开(公告)日：2019-05-09
申请号：US16246864
申请日：2019-01-14
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER
Abstract: As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.
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公开(公告)号：US20180142250A1
公开(公告)日：2018-05-24
申请号：US15867770
申请日：2018-01-11
Applicant: DOW AGROSCIENCES LLC
Inventor： Lakshmi SASTRY-DENT , Zehui CAO , Shreedharan SRIRAM , Steven R. WEBB , Debra L. CAMPER , W. Michael AINLEY
CPC classification number: C12N15/8213 , A01H5/10 , C12N15/8243 , C12N15/8273 , C12N15/8274 , C12N15/8286
Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.
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