公开(公告)号：US20150064708A1

公开(公告)日：2015-03-05

申请号：US14475968

申请日：2014-09-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael AINLEY ,  Jayakumar P. SAMUEL ,  Zehui CAO ,  Liu Y. SHEN ,  Cristie M. DEWES

IPC: C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座上的供体DNA多核苷酸插入物的高通量过程。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。

公开(公告)号：US20210230617A1

公开(公告)日：2021-07-29

申请号：US17224659

申请日：2021-04-07

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi SASTRY-DENT ,  Zehui CAO ,  Shreedharan SRIRAM ,  Steven R. WEBB ,  Debra L. CAMPER ,  W. Michael AINLEY

IPC: C12N15/82 ,  A01H5/10

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20180142250A1

公开(公告)日：2018-05-24

申请号：US15867770

申请日：2018-01-11

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi SASTRY-DENT ,  Zehui CAO ,  Shreedharan SRIRAM ,  Steven R. WEBB ,  Debra L. CAMPER ,  W. Michael AINLEY

IPC: C12N15/82 ,  A01H5/10

CPC classification number: C12N15/8213 ,  A01H5/10 ,  C12N15/8243 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8286

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US20180010200A1

公开(公告)日：2018-01-11

申请号：US15705447

申请日：2017-09-15

Applicant: DOW AGROSCIENCES LLC

Inventor： Lakshmi SASTRY-DENT ,  W. Michael AINLEY ,  Jayakumar P. SAMUEL ,  Zehui CAO ,  Liu SHEN ,  Cristie M. DEWES

IPC: C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

公开(公告)号：US20170044558A1

公开(公告)日：2017-02-16

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David R. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82 ,  C12N9/12

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。

公开(公告)号：US20150128308A1

公开(公告)日：2015-05-07

申请号：US14531748

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi SASTRY-DENT ,  Zehui CAO ,  Shreedharan SRIRAM ,  Steven R. WEBB ,  Debra L. CAMPER ,  W. Michael AINLEY

IPC: C12N15/82 ,  A01H5/10

CPC classification number: C12N15/8213 ,  A01H5/10 ,  C12N15/8243 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8286

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Abstract translation: 如本文所公开的，已经鉴定出代表用于靶向插入外源序列的最佳位点的大豆植物的最佳天然基因组位点。

公开(公告)号：US20140173783A1

公开(公告)日：2014-06-19

申请号：US14105563

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David H. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32中的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。