公开(公告)号：US20150059021A1

公开(公告)日：2015-02-26

申请号：US14381985

申请日：2013-02-28

申请人： DOW AGROSCIENCES LLC

发明人： Patricia Ann Owens Merlo ,  Cory Larsen ,  Scott A. Bevan ,  John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  Mark Allen Thompson

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

摘要翻译： 鉴定新的增强子序列在植物功能基因组学中具有显着的效用。 已经鉴定出甘蔗杆状坏死病毒（SCBV）转录增强子。 该增强子可用于增加基因启动子的转录速率和激活标记实验。 当将4×阵列的SCBV增强子置于玉米醇脱氢酶最小启动子的截短形式的上游时，观察到转录增加10倍。 描述了使用SCBV转录增强子的方法，嵌合转录调控区，构建体，细胞，组织和包含增强子一个或多个拷贝的生物体也是如此。

公开(公告)号：US20140287407A1

公开(公告)日：2014-09-25

申请号：US14204957

申请日：2014-03-11

申请人： DOW AGROSCIENCES LLC

发明人： John P. Davies ,  Warren S. Lee ,  Vaka S. Reddy ,  Xing Liang Liu ,  Satyalinga S. Gampala

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895 ,  C12Q1/6816 ,  C12Q2563/179

摘要： This disclosure concerns a system and method for detecting heterologous DNA in plant materials.

摘要翻译： 本公开涉及用于在植物材料中检测异源DNA的系统和方法。

公开(公告)号：US09896692B2

公开(公告)日：2018-02-20

申请号：US14570823

申请日：2014-12-15

申请人： Dow AgroSciences LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  D. Ry Wagner ,  Xing Liang Liu

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8209 ,  C12N15/8205 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

摘要： An activation tagging construct for maize that includes one or more sugarcane bacilliform viral (SCBV) enhancer elements, and resulting tagged populations and plants, are described. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

公开(公告)号：US20140289899A1

公开(公告)日：2014-09-25

申请号：US14302286

申请日：2014-06-11

申请人： Dow Agrosciences LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  A01H5/10 ,  C12N15/113 ,  C12N15/8241 ,  C12N15/8261 ,  Y02A40/146

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

摘要翻译： 鉴定新的增强子序列在植物功能基因组学中具有显着的效用。 已经鉴定出甘蔗杆状坏死病毒（SCBV）转录增强子。 该增强子可用于增加基因启动子的转录速率和激活标记实验。 当将4×阵列的SCBV增强子置于玉米醇脱氢酶最小启动子的截短形式的上游时，观察到转录增加10倍。 描述了使用SCBV转录增强子的方法，嵌合转录调控区，构建体，细胞，组织和包含增强子一个或多个拷贝的生物体也是如此。

公开(公告)号：US10227598B2

公开(公告)日：2019-03-12

申请号：US15154195

申请日：2016-05-13

申请人： Dow Agrosciences LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

IPC分类号： C12N15/113 ,  C12N15/82 ,  A01H5/10

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

公开(公告)号：US10077450B2

公开(公告)日：2018-09-18

申请号：US14381985

申请日：2013-02-28

申请人： DOW AGROSCIENCES LLC

发明人： Patricia Ann Owens Merlo ,  Cory Larsen ,  Scott A. Bevan ,  John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  Mark Allen Thompson

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N15/8234

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

公开(公告)号：US10017828B2

公开(公告)日：2018-07-10

申请号：US14204957

申请日：2014-03-11

申请人： DOW AGROSCIENCES LLC

发明人： John P. Davies ,  Warren S. Lee ,  Vaka S. Reddy ,  Xing Liang Liu ,  Satyalinga S. Gampala

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12Q1/6895 ,  C12Q1/6816

CPC分类号： C12Q1/6895 ,  C12Q1/6816 ,  C12Q2563/179

摘要： This disclosure concerns a system and method for detecting heterologous DNA in plant materials.

公开(公告)号：US20160251733A1

公开(公告)日：2016-09-01

申请号：US15154195

申请日：2016-05-13

申请人： Dow Agrosciences LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

IPC分类号： C12Q1/70 ,  C12N15/82

CPC分类号： C12N15/8216 ,  A01H5/10 ,  C12N15/113 ,  C12N15/8241 ,  C12N15/8261 ,  Y02A40/146

摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

公开(公告)号：US20150096080A1

公开(公告)日：2015-04-02

申请号：US14570823

申请日：2014-12-15

申请人： DOW AGROSCIENCES LLC

发明人： John P. Davies ,  Vaka S. Reddy ,  William Michael Ainley ,  D. Ry Wagner ,  Xing Liang Liu

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8209 ,  C12N15/8205 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/16

摘要： Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.

摘要翻译： 本文公开了一种用于玉米的激活标签构建体，得到标签种群和植物。 在一个实例中，激活标记DNA构建体包括转座酶的编码序列，可检测的报道分子（如花青素调节基因B-Peru和C1）和非自主转座T-DNA盒。 例如，将可转座T-DNA盒插入可检测的报道编码区，使得B-Peru和C1基因仅在切换转座盒时才在含有玉米激活标记DNA构建体的细胞中表达花青素。 产生标记的玉米植物群的方法包括用所公开的构建体转化玉米植物细胞或组织。