公开(公告)号：US20160208272A1

公开(公告)日：2016-07-21

申请号：US14913614

申请日：2014-08-20

申请人： E. I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Andrew Cigan ,  Saverio Carl Falco ,  Huirong GAO ,  Zhongsen Li ,  Zhan-Bin Liu ,  L. Aleksander Lyznik ,  Jinrui Shi ,  Sergei Svitashev ,  Joshua K. Young

IPC分类号： C12N15/82 ,  A01H1/02

CPC分类号： C12N15/8216 ,  C12N15/113 ,  C12N15/81 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12N2310/321 ,  C12N2310/3521 ,  C12N2310/322 ,  C12N2310/3533

摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

公开(公告)号：US20150082478A1

公开(公告)日：2015-03-19

申请号：US14463687

申请日：2014-08-20

申请人： E I DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL INC

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  L. Aleksander Lyznik ,  Jinrui Shi ,  Sergei Svitashev ,  Joshua K. Young

IPC分类号： C12N15/82 ,  C12Q1/68 ,  C12N15/01 ,  A01H1/02 ,  A01H1/04

CPC分类号： C12N15/8216 ,  C12N15/113 ,  C12N15/81 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8261 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8279 ,  C12N15/8286 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12N2310/321 ,  C12N2310/3521 ,  C12N2310/322 ,  C12N2310/3533

摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

摘要翻译： 提供组合物和方法用于基因组修饰植物或植物细胞基因组中的靶序列。 方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。 还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于基因组修饰细胞或生物体基因组中的核苷酸序列，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法 的细胞或生物体。 一旦识别出基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。 还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。 还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。

公开(公告)号：US20180371479A1

公开(公告)日：2018-12-27

申请号：US16054156

申请日：2018-08-03

申请人： E. I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Andrew Mark Cigan ,  Saverio Carl Falco ,  Huirong Gao ,  Zhongsen Li ,  Zhan-Bin Liu ,  L. Aleksander Lyznik ,  Jinrui Shi ,  Sergei Svitashev ,  Joshua K. Young

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N15/81 ,  C12Q1/6895

摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

公开(公告)号：US20150159166A1

公开(公告)日：2015-06-11

申请号：US14355249

申请日：2012-10-29

申请人： PIONEER HI BRED INTERNATIONAL INC

发明人： Rayeann Archibald ,  Wesley Bruce ,  Mei Guo ,  Rajeev Gupta ,  Mary Rupe ,  Kathleen Schellin ,  Jinrui Shi ,  Carl Simmons

IPC分类号： C12N15/82 ,  C07K14/415 ,  A01H1/04

CPC分类号： C12N15/8249 ,  A01H1/04 ,  C07K14/415 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  Y02A40/146

摘要： The present disclosure provides polynucleotides and related polypeptides which are used to modify ethylene sensitivity in plants. Ethylene insensitive transgenic maize plants produce higher grain yields in water deficient and low nitrogen environments than non-transgenic plants. Through controlled expression of the transgene in desired tissues and organs, or specific plant developmental stages, the ethylene perception and signal transduction is altered to create transgenic plants which yield better under abiotic stress.

摘要翻译： 本公开提供了用于修饰植物中乙烯敏感性的多核苷酸和相关多肽。 乙烯不敏感的转基因玉米植物在缺水和低氮环境中比非转基因植物产生更高的谷物产量。 通过将转基因在期望的组织和器官或特定植物发育阶段中的受控表达，乙烯感知和信号转导被改变以产生在非生物胁迫下产生更好的转基因植物。