公开(公告)号：US20150275173A1

公开(公告)日：2015-10-01

申请号：US14436206

申请日：2013-10-29

Applicant: EISAI R&D MANAGEMENT CO., LTD.

Inventor： Norimasa Miyamoto ,  Yuichi Ono ,  Kana Namiki ,  Yoshitoshi Kasuya

IPC: C12N5/0797

CPC classification number: C12N5/0623 ,  C12N15/85 ,  C12N2500/14 ,  C12N2501/40 ,  C12N2501/405 ,  C12N2501/998 ,  C12N2506/00 ,  C12N2510/00

Abstract: The object of the present invention is to provide a neural stem cell having increased passage ability, a method for manufacturing a neural stem cell having said increased passage ability, and others. The present invention, in one embodiment, provides a neural stem cell having increased passage ability having the following characteristics: (a) the N-type calcium channel gene is knocked out or knocked down in said cell, (b) the influx of Ca2+ via the N-type calcium channel is substantially absent or suppressed in said cell, (c) said cell can be passaged for at least 4 generations (more preferably 15 generations) or more, and (d) said cell maintains the differentiation potential into a nerve cell even after passage for 4 generations (more preferably 15 generations).

Abstract translation: 本发明的目的是提供具有增加的通过能力的神经干细胞，具有所述增加的通过能力的神经干细胞的制造方法等。 在一个实施方案中，本发明提供具有增加的通过能力的神经干细胞，其具有以下特征：（a）N型钙通道基因在所述细胞中被敲除或敲低，（b）Ca 2+通过 所述细胞中N型钙通道基本上不存在或被抑制，（c）所述细胞可传代至少4代（更优选15代）或更多，和（d）所述细胞维持分化潜能为神经 细胞即使经过4代（更优选15代）。

公开(公告)号：US09574176B2

公开(公告)日：2017-02-21

申请号：US14436206

申请日：2013-10-29

Applicant: Eisai R&D Management Co., Ltd.

Inventor： Norimasa Miyamoto ,  Yuichi Ono ,  Kana Namiki ,  Yoshitoshi Kasuya

IPC: A61K48/00 ,  C12N5/00 ,  C12N5/08 ,  C12N5/0797 ,  C12N15/85

CPC classification number: C12N5/0623 ,  C12N15/85 ,  C12N2500/14 ,  C12N2501/40 ,  C12N2501/405 ,  C12N2501/998 ,  C12N2506/00 ,  C12N2510/00

Abstract: The present invention provides a neural stem cell having increased passage ability and a method for manufacturing a neural stem cell having increased passage ability. A neural stem cell in which the N-type calcium channel gene is knocked out or the influx of Ca2+ via the N-type calcium channel is substantially absent can be passaged for at least 4 generations and maintains the differentiation potential into a nerve cell even after passage for 4 generations.

Abstract translation: 本发明提供了具有增加的通过能力的神经干细胞和具有增加的通过能力的神经干细胞的制造方法。 其中N型钙通道基因被敲除或通过N型钙通道Ca2 +的流入基本上不存在的神经干细胞可以传代至少4代并且即使在 通过4代。