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    NEURAL STEM CELL HAVING INCREASED PASSAGE ABILITY, METHOD FOR MANUFACTURING NEURAL STEM CELL HAVING SAID INCREASED PASSAGE ABIILITY, AND METHOD FOR CULTURING NEURAL STEM CELLS FOR INCREASING PASSAGE ABILITY OF NEURAL STEM CELLS
    1.
    发明申请
    NEURAL STEM CELL HAVING INCREASED PASSAGE ABILITY, METHOD FOR MANUFACTURING NEURAL STEM CELL HAVING SAID INCREASED PASSAGE ABIILITY, AND METHOD FOR CULTURING NEURAL STEM CELLS FOR INCREASING PASSAGE ABILITY OF NEURAL STEM CELLS 有权
    具有增加的呼吸能力的神经干细胞，用于制造具有提高的呼吸功能的神经干细胞的方法，以及用于培养神经干细胞以增加神经干细胞的通过能力的方法

    公开(公告)号：US20150275173A1

    公开(公告)日：2015-10-01

    申请号：US14436206

    申请日：2013-10-29

    Applicant: EISAI R&D MANAGEMENT CO., LTD.

    Inventor： Norimasa Miyamoto ,  Yuichi Ono ,  Kana Namiki ,  Yoshitoshi Kasuya

    IPC: C12N5/0797

    CPC classification number: C12N5/0623 ,  C12N15/85 ,  C12N2500/14 ,  C12N2501/40 ,  C12N2501/405 ,  C12N2501/998 ,  C12N2506/00 ,  C12N2510/00

    Abstract: The object of the present invention is to provide a neural stem cell having increased passage ability, a method for manufacturing a neural stem cell having said increased passage ability, and others. The present invention, in one embodiment, provides a neural stem cell having increased passage ability having the following characteristics: (a) the N-type calcium channel gene is knocked out or knocked down in said cell, (b) the influx of Ca2+ via the N-type calcium channel is substantially absent or suppressed in said cell, (c) said cell can be passaged for at least 4 generations (more preferably 15 generations) or more, and (d) said cell maintains the differentiation potential into a nerve cell even after passage for 4 generations (more preferably 15 generations).

    Abstract translation: 本发明的目的是提供具有增加的通过能力的神经干细胞，具有所述增加的通过能力的神经干细胞的制造方法等。 在一个实施方案中，本发明提供具有增加的通过能力的神经干细胞，其具有以下特征：（a）N型钙通道基因在所述细胞中被敲除或敲低，（b）Ca 2+通过 所述细胞中N型钙通道基本上不存在或被抑制，（c）所述细胞可传代至少4代（更优选15代）或更多，和（d）所述细胞维持分化潜能为神经 细胞即使经过4代（更优选15代）。

    METHOD FOR INDUCING DOPAMINERGIC NEURON PROGENITOR CELLS
    2.
    发明申请
    METHOD FOR INDUCING DOPAMINERGIC NEURON PROGENITOR CELLS 审中-公开
    诱导多巴胺神经元原核细胞的方法

    公开(公告)号：US20160215260A1

    公开(公告)日：2016-07-28

    申请号：US14916696

    申请日：2014-09-04

    Applicant: KYOTO UNIVERSITY ,  OSAKA UNIVERSITY ,  EISAI R&D MANAGEMENT CO., LTD.

    Inventor： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Kiyotoshi Sekiguchi ,  Yuichi Ono

    IPC: C12N5/0797 ,  A61K35/30

    CPC classification number: C12N5/0623 ,  A61K35/30 ,  C12N5/0619 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/45 ,  C12N2533/52

    Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.

    Abstract translation: 本发明提供了一种用于从多能干细胞产生多巴胺能神经元祖细胞的方法，该方法包括以下步骤：（i）在含有选自以下的试剂的培养基中，在细胞外基质上进行多能干细胞的贴壁培养： 由BMP抑制剂，TGFβ抑制剂，SHH信号刺激剂，FGF8和GSK3β抑制剂组成的组; （ii）使用与Corin结合的物质和/或与Lrtm1结合的物质从步骤（i）中获得的细胞收集Corin和/或Lrtm1阳性细胞; 和（iii）在含有神经营养因子的培养基中进行步骤（ii）中获得的细胞的悬浮培养。

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