公开(公告)号：US20210387184A1

公开(公告)日：2021-12-16

申请号：US17218027

申请日：2021-03-30

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE ,  Sinan ARSLAN ,  Molly HE ,  Huizhen MAH ,  Lei SUN

IPC: B01L3/00 ,  C12Q1/6874 ,  C12N15/10 ,  C12Q1/6806 ,  G01N21/64

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20210333210A1

公开(公告)日：2021-10-28

申请号：US17372359

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: G01N21/64 ,  C12Q1/6869 ,  G01N15/14

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20210123098A1

公开(公告)日：2021-04-29

申请号：US17144945

申请日：2021-01-08

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve CHEN

IPC: C12Q1/6869 ,  C12Q1/6841

Abstract: Provided are methods and systems for analyzing nucleic acids in a biological sample in a manner that retains the spatial and/or cellular origin of the nucleic acids within the biological sample. Compositions and kits are also provided that enable the methods and systems of the instant disclosure.

公开(公告)号：US20220405523A1

公开(公告)日：2022-12-22

申请号：US17854042

申请日：2022-06-30

Applicant: Element Biosciences, Inc.

Inventor： Chunhong ZHOU ,  Semyon KRUGLYAK ,  Francisco GARCIA ,  Minghao GUO ,  Haosen WANG ,  Ryan KELLY

IPC: G06K9/62 ,  G06T7/30 ,  G06V10/75 ,  G06V20/69

Abstract: Methods and systems for image analysis are provided, and in particular for identifying a set of base-calling locations in a flow cell for DNA sequencing. These include capturing flow cell images after each sequencing step performed on the flow cell, and identifying candidate cluster centers in at least one of the flow cell images. Intensities are determined for each candidate cluster center in a set of flow cell images. Purities are determined for each candidate cluster center based on the intensities. Each candidate cluster center with a purity greater than the purity of the surrounding candidate cluster centers within a distance threshold is added to a template set of base-calling locations.

公开(公告)号：US20220170919A1

公开(公告)日：2022-06-02

申请号：US17675154

申请日：2022-02-18

Applicant: Element Biosciences, Inc.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve Xiangling CHEN

IPC: G01N33/53 ,  G01N33/58 ,  C12Q1/6874

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20210333211A1

公开(公告)日：2021-10-28

申请号：US17372362

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: G01N21/64 ,  C12Q1/6869 ,  G01N15/14

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20210222238A1

公开(公告)日：2021-07-22

申请号：US17016349

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20210139981A1

公开(公告)日：2021-05-13

申请号：US17156452

申请日：2021-01-22

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan ARSLAN ,  Chunhong ZHOU ,  Molly Min HE ,  Matthew KELLINGER ,  Adeline Huizhen MAH ,  Michael PREVITE ,  Lei SUN

IPC: C12Q1/6876 ,  G01N33/543 ,  C12N9/12 ,  G01N21/64

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20240382956A1

公开(公告)日：2024-11-21

申请号：US18680203

申请日：2024-05-31

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20230326064A1

公开(公告)日：2023-10-12

申请号：US18078797

申请日：2022-12-09

Applicant: Element Biosciences, Inc.

Inventor： Chunhong ZHOU ,  Semyon KRUGLYAK ,  Francisco GARCIA ,  Minghao GUO ,  Haosen WANG ,  Ryan KELLY

IPC: G06T7/66 ,  G06T7/30 ,  G06V10/75 ,  G06V20/69 ,  G06F18/232 ,  G06V10/762

CPC classification number: G06T7/66 ,  G06T7/30 ,  G06V10/751 ,  G06V20/693 ,  G06V20/695 ,  G06F18/232 ,  G06V10/763 ,  G06V20/69 ,  G06V2201/04

Abstract: Image data analysis, particularly identifying cluster locations for performing base-calling in a digital flow cell image during DNA sequencing, is described. Each nucleic acid template molecule immobilized on a support may include an insert sequence and a sample index sequence. The sample index sequence may include a k-mer sequence. A sequencing system may conduct k cycles of sequencing reactions of the k-mer sequence before conducting one or more cycles of the insert sequence sequencing reactions and generate a first plurality of flow cell images. Pixel intensities may be determined for pixels of the first plurality of flow cell images. A base calling template may be determined and include base calling locations based on the pixel intensities and respective color purities of the pixel intensities. The base calling template may register a second plurality of flow cell images of the support in one or more cycles subsequent to the k cycles.