公开(公告)号：US09891218B2

公开(公告)日：2018-02-13

申请号：US15006153

申请日：2016-01-26

Applicant: EMD Millipore Corporation

Inventor： Chris Scott ,  Phillip Clark ,  Kurt E. Greenizen ,  Ryan A. Amara ,  Robert Colonna

IPC: G01N21/75 ,  G01N33/558 ,  G01N33/543 ,  B01L3/00 ,  B01L9/00 ,  G01N33/569 ,  B01J20/28

CPC classification number: G01N33/558 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/2804 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2469/10 ,  G01N2469/20

Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

公开(公告)号：US20160178625A1

公开(公告)日：2016-06-23

申请号：US15006153

申请日：2016-01-26

Applicant: EMD Millipore Corporation

Inventor： Chris Scott ,  Phillip Clark ,  Kurt E. Greenizen ,  Ryan A. Amara ,  Robert Colonna

IPC: G01N33/558 ,  B01L9/00 ,  B01L3/00

CPC classification number: G01N33/558 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/2804 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/12 ,  B01L2300/126 ,  B01L2400/0487 ,  B01L2400/049 ,  G01N33/54366 ,  G01N33/56911 ,  G01N2469/10 ,  G01N2469/20

Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.

Abstract translation: 本文公开的实施方案涉及用于在印迹膜上进行化合物检测的装置。 该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许 降低这种液体的起始体积。 优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。 分配器和支撑件保持在一起以在膜周围形成一个信封。 在这样做时优选使用铰链，夹子和其它这样的装置。