公开(公告)号：US20130330821A1

公开(公告)日：2013-12-12

申请号：US13874507

申请日：2013-05-01

申请人： ES Cell International PTE Ltd.

发明人： BENJAMIN EITHAN REUBINOFF ,  MARTIN Federick PERA ,  TAMIR BEN-HUR

IPC分类号： C12N5/0797

CPC分类号： C12N5/0623 ,  A61K35/12 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/91 ,  C12N2502/13 ,  C12N2506/02

摘要： The present invention relates to undifferentiated human embryonic stem cells, methods of cultivation and propagation and production of differentiated cells. In particular it relates to the production of human ES cells capable of yielding somatic differentiated cells in vitro, as well as committed progenitor cells such as neural progenitor cells capable of giving rise to mature somatic cells including neural cells and/or glial cells and uses thereof. This invention provides methods that generate in vitro and in vivo models of controlled differentiation of ES cells towards the neural lineage. The model, and cells that are generated along the pathway of neural differentiation may be used for: the study of the cellular and molecular biology of human neural development, discovery of genes, growth factors, and differentiation factors that play a role in neural differentiation and regeneration, drug discovery and the development of screening assays for teratogenic, toxic and neuroprotective effects.

摘要翻译： 本发明涉及未分化的人类胚胎干细胞，培养和繁殖方法以及分化细胞的产生。 特别地，它涉及能够在体外产生体细胞分化细胞的人ES细胞的产生，以及确定的祖细胞，例如能够产生包括神经细胞和/或胶质细胞的成熟体细胞的神经祖细胞及其用途 。 本发明提供了产生ES细胞向神经谱系的受控分化的体外和体内模型的方法。 沿着神经分化途径产生的模型和细胞可用于：人类神经发育的细胞和分子生物学的研究，发现基因，生长因子和在神经分化中发挥作用的分化因子， 再生，药物发现和开发用于致畸，毒性和神经保护作用的筛选试验。