公开(公告)号：US10351891B2

公开(公告)日：2019-07-16

申请号：US15535274

申请日：2014-12-12

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Fengqing Wang ,  Dongzhi Wei ,  Liqin Xu ,  Haohao Liu ,  Xinbin Liu ,  Nenghui Chen

IPC: C12P33/02 ,  C12P33/00 ,  C12N9/10 ,  C12N9/04 ,  C12R1/32

Abstract: The present invention provides a hydroxyacyl-coenzyme A dehydrogenase gene, an acyl-coenzyme A thiolase gene, genetically engineered strains and a use thereof. The hydroxyacyl-coenzyme A dehydrogenase gene encodes a protein (i) or (ii) as follows: (i) having an amino acid sequence according to SEQ ID NO 2; (ii) derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by (i) and having the same function as that of the protein of (i). The present invention constructs genetically engineered Mycobacterium strains lacking of a hydroxyacyl-coenzyme A dehydrogenase gene or an acyl-coenzyme A thiolase gene, which are used in the preparation of steroidal compounds, such as 1,4-BNA, 4-BNA, 9-OH-BNA, etc. Further, the invention improves the production efficiency and product quality of steroidal drug, improves the utilization of drug precursors, reduces the production costs, and provides the advantages of mild reaction conditions, environmentally friendly, and high economic and social benefits.

公开(公告)号：US10774355B2

公开(公告)日：2020-09-15

申请号：US16310741

申请日：2016-06-16

Applicant: East China University of Science and Technology

Inventor： Fengqing Wang ,  Liqin Xu ,  Yongjun Liu ,  Haohao Liu ,  Liangbin Xiong ,  Dongzhi Wei

IPC: C12P33/00 ,  C12P33/02 ,  C12N9/02 ,  C12R1/325 ,  C12R1/33 ,  C12R1/34 ,  C12R1/32

Abstract: The present invention relates to a genetically-engineered Mycobacterium strain and a use thereof in the preparation of steroidal compounds. The genetically-engineered Mycobacterium strain is a Mycobacteria which lacks of acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33, wherein acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33 respectively encode proteins as follows: having amino acid sequences according to SEQ ID NOs 4, 6 and 8; derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by preceding protein and having the same function as that of the preceding protein. The present invention constructs a genetically-engineered Mycobacterium strain and applies it in preparing steroidal compounds, thereby enriching the types of valuable intermediates, improving the production efficiency and product quality of steroid drugs, reducing energy consumption in the steroid drugs production, simplifying production steps, and reducing production costs.

公开(公告)号：US20190264249A1

公开(公告)日：2019-08-29

申请号：US16310741

申请日：2016-06-16

Applicant: East China University Of Science And Technology

Inventor： Fengqing Wang ,  Liqin Xu ,  Yongjun Liu ,  Haohao Liu ,  Liangbin Xiong ,  Dongzhi Wei

IPC: C12P33/00 ,  C12R1/34 ,  C12R1/33 ,  C12R1/325 ,  C12N9/02

Abstract: The present invention relates to a genetically-engineered Mycobacterium strain and a use thereof in the preparation of steroidal compounds. The genetically-engineered Mycobacterium strain is a Mycobacteria which lacks of acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33, wherein acyl-CoA dehydrogenase genes fadE31, fadE32 and fadE33 respectively encode proteins as follows: having amino acid sequences according to SEQ ID NOs 4, 6 and 8; derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by preceding protein and having the same function as that of the preceding protein. The present invention constructs a genetically-engineered Mycobacterium strain and applies it in preparing steroidal compounds, thereby enriching the types of valuable intermediates, improving the production efficiency and product quality of steroid drugs, reducing energy consumption in the steroid drugs production, simplifying production steps, and reducing production costs.