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12 条记录 (耗时 0.028 秒)

    TUNNEL JUNCTIONS IN MICROFLUIDIC ARRAYS FOR MOLECULAR RECOGNITION
    4.
    发明申请
    TUNNEL JUNCTIONS IN MICROFLUIDIC ARRAYS FOR MOLECULAR RECOGNITION 审中-公开

    公开(公告)号：US20180031523A1

    公开(公告)日：2018-02-01

    申请号：US15661931

    申请日：2017-07-27

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Yann Astier ,  Juraj Topolancik

    IPC分类号： G01N27/447 ,  B01L3/00 ,  H01L43/12 ,  H01L43/02 ,  H01L43/10

    摘要： Embodiments of the present technology include a system for analyzing a molecule. The system may include a device. The device includes a first conductive element, a second conductive element, and an insulating layer. The insulating layer may be tapered in a direction to reach a minimum thickness at a first end of the device. The insulating layer is disposed between the first conductive element and the second conductive element. The device may include a voltage source in electrical communication with the first conductive element and the second conductive element. The device may also include an electrical meter in electrical communication with the voltage source, the first conductive element, and the second conductive element.

    Nucleic acid sequencing using nanotransistors
    6.
    发明授权
    Nucleic acid sequencing using nanotransistors 有权

    公开(公告)号：US11345961B2

    公开(公告)日：2022-05-31

    申请号：US16337847

    申请日：2017-09-28

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Yann Astier

    IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6823

    摘要： Embodiments may include a method of determining a nucleic acid sequence. The nucleic acid may be DNA. The method may include forming, by a polymerase, a double-stranded nucleic acid molecule from a first nucleic acid strand and a second nucleic acid strand. Forming the double-stranded nucleic acid molecule may include adding a first compound to the second nucleic acid strand. The first compound may include a nucleotide attached to a third nucleic acid strand. The third nucleic acid strand may be attached to a moiety. The method may also include detaching the nucleotide from the third nucleic acid strand and the moiety. The method may further include measuring a change in an electrical characteristic of a transistor resulting from the moiety. Additionally, the method may include identifying the nucleotide based on the measured change in the electrical characteristic.

    Methods and systems for nucleic acid sequencing by tunneling recognition
    7.
    发明授权
    Methods and systems for nucleic acid sequencing by tunneling recognition 有权

    公开(公告)号：US11624091B2

    公开(公告)日：2023-04-11

    申请号：US16839875

    申请日：2020-04-03

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Yann Astier

    IPC分类号： C12Q1/6869 ,  G01N27/327 ,  G01N33/487

    摘要： Nucleic acid molecule analysis systems are described. The system may include a nucleic acid molecule attached to a particle with a characteristic dimension. The system may also include an aperture defined by a first electrode, a first insulator, and a second electrode. The aperture may have a characteristic dimension less than the characteristic dimension of the particle. The system may further include a first power supply in electrical communication with the first electrode and the second electrode. In addition, the system may include a second power supply configured to apply an electric field through the aperture. In some embodiments, the aperture may be defined by a first insulator. A portion of the first electrode may extend into the aperture. A portion of the second electrode may extend into the aperture.

    METHODS OF SEQUENCING BY SYNTHESIS USING A CONSECUTIVE LABELING SCHEME
    8.
    发明申请
    METHODS OF SEQUENCING BY SYNTHESIS USING A CONSECUTIVE LABELING SCHEME 有权

    公开(公告)号：US20230061438A1

    公开(公告)日：2023-03-02

    申请号：US17757527

    申请日：2020-12-17

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Yann Astier ,  Frank Bergmann ,  Dieter Heindl

    IPC分类号： C12Q1/6874 ,  C12Q1/6837 ,  C07H19/24

    摘要： The present disclosure provides a method for sequencing target polynucleotide molecules. In some embodiments, the present disclosure provides a method of sequencing by synthesis where different subsets of nucleotide-conjugate complexes are sequentially formed and detected during each iterative extension of a plurality of nascent nucleic acid copy strands, where each nascent nucleic acid copy strand is complementary to one of a plurality of target polynucleotide molecules. In some embodiments, the plurality of target polynucleotide molecules are arrayed on a solid support.

    Methods and systems for nucleic acid sequencing by tunneling recognition
    10.
    发明授权
    Methods and systems for nucleic acid sequencing by tunneling recognition 有权

    公开(公告)号：US10648029B2

    公开(公告)日：2020-05-12

    申请号：US16218938

    申请日：2018-12-13

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Yann Astier

    IPC分类号： C12Q1/6869 ,  G01N27/327 ,  G01N33/487

    摘要： Embodiments may include a method of analyzing a nucleic acid molecule. The method may include attaching the nucleic acid molecule to a protein. The protein may be attached to a particle with a first diameter. The method may also include applying an electric field to move a first portion of the nucleic acid molecule into an aperture. The aperture may be defined by a first electrode, an insulator, and a second electrode. The aperture may have a second diameter less than the first diameter. The method may further include contacting the first portion of the nucleic acid molecule to both the first electrode and the second electrode. The method may include applying a voltage across the first electrode and the second electrode. The current through the electrodes and the portion of the nucleic acid molecule may be measured, and a nucleotide of the nucleic acid molecule may be identified.