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公开(公告)号：US4803167A
公开(公告)日：1989-02-07
申请号：US363545
申请日：1982-03-30
摘要： The present invention discloses a hybridoma culture and high affinity monoclonal antidigoxin antibodies produced by somatic cell fusion. The antibodies are useful for therapeutic as well as for diagnostic purposes. They are also useful in a model system for the study of structure-function relationships in antigen-antibody specific reactions.
摘要翻译： 本发明公开了通过体细胞融合产生的杂交瘤培养物和高亲和力单克隆抗体素抗体。 抗体可用于治疗和诊断目的。 它们也可用于研究抗原 - 抗体特异性反应中结构 - 功能关系的模型系统。
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公开(公告)号：US4792601A
公开(公告)日：1988-12-20
申请号：US656482
申请日：1984-10-01
IPC分类号： C07K16/00 , C07K16/18 , C12N5/16 , G01N33/53 , A61K39/395 , C12N5/00 , C12N15/00 , C12P21/00
摘要： In a method for preparing a monoclonal hybridoma which secretes antibodies against an immunogenic material, comprising sensitizing an appropriate source with an immunizing amount of a preparation of the immunogenic material, obtaining from the source lymphocytes having immune activity against the immunogenic material, and fusing the lymphocytes with an appropriate cell line to thereby form mixed hybridomas, the improvement wherein: the immunogenic material is present in the immunizing preparation in an amount not larger than 10% by weight, and wherein, after formation of the mixed hydridomas but prior to cloning, the method comprises the steps of: raising ascites fluid with the mixed hydridomas, then detecting immune specificity against the immunogenic material in the ascites raised fluid; and thereafter cloning the ascites raised cells which show immune specificity against the material.
摘要翻译： 在制备分泌针对免疫原性物质的抗体的单克隆杂交瘤的方法中，包括用免疫量的免疫原性物质的制剂对适当的来源进行敏化，从源获得具有针对免疫原性物质的免疫活性的淋巴细胞，并融合淋巴细胞 用合适的细胞系形成混合的杂交瘤，其改进在于：免疫原性物质以不大于10重量％的量存在于免疫制剂中，并且其中，在形成混合的hydridomas之后但是在克隆之前， 方法包括以下步骤：用混合的hydridomas提升腹水，然后检测腹水引起的流体中对免疫原性物质的免疫特异性; 然后克隆显示对材料的免疫特异性的腹水升高的细胞。
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公开(公告)号：US4487833A
公开(公告)日：1984-12-11
申请号：US353089
申请日：1982-03-01
摘要： In a method for preparing a monoclonal hybridoma which secretes antibodies against an immunogenic material, comprising sensitizing an appropriate source with an immunizing amount of a preparation of the immunogenic material, obtaining from the source lymphocytes having immune activity against the immunogenic material, and fusing the lymphocytes with an appropriate cell line to thereby form mixed hybridomas, the improvement wherein: the immunogenic material is present in the immunizing preparation in an amount not larger than 5% by weight, and wherein, after formation of the mixed hybridomas but prior to cloning, the method comprises the steps of: raising ascites fluid with the mixed hybridomas, then detecting immune specificity against the immunogenic material in the ascites raised fluid; and thereafter cloning the ascites raised cells which show immune specificity against the material.
摘要翻译： 在制备分泌针对免疫原性物质的抗体的单克隆杂交瘤的方法中，包括用免疫量的免疫原性物质的制剂对适当的来源进行敏化，从源获得具有针对免疫原性物质的免疫活性的淋巴细胞，并融合淋巴细胞 用适当的细胞系形成混合的杂交瘤，其改进在于：免疫原性物质以不超过5％的量存在于免疫制剂中，并且其中，在形成混合杂交瘤之后但是在克隆之前， 方法包括以下步骤：用混合杂交瘤提升腹水，然后检测腹水引起的流体中对免疫原性物质的免疫特异性; 然后克隆显示对材料的免疫特异性的腹水升高的细胞。
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