公开(公告)号：US20060243899A1

公开(公告)日：2006-11-02

申请号：US11028230

申请日：2005-01-04

申请人： Eiichi Matsuo ,  Chikako Toda ,  Makoto Watanabe ,  Osamu Nishimura ,  Noriyuki Ojima

发明人： Eiichi Matsuo ,  Chikako Toda ,  Makoto Watanabe ,  Osamu Nishimura ,  Noriyuki Ojima

IPC分类号： B01D59/44

CPC分类号： H01J49/0418 ,  G01N33/6842 ,  G01N33/6851

摘要： The present invention provides a method which is capable of mainly detecting the objective substance by mass spectrometry and can also be applied effectively to a sample to be measured without conducting enrichment process. A mass spectrometric method comprising: in a mass spectrometry specifically ionizing a specific substance to be measured contained in a mixture sample containing the specific substance and a substance other than the specific substance by using a matrix that is more likely to ionize the specific substance than the substance other than the specific substance, to selectively measure the specific substance from the mixture. Preferably, the specific substance is a peptide modified with 2-nitrobenzenesulfenyl chloride, and the matrix is a nitrobenzene derivative such as hydroxynitrobenzoic acid. The matrix is preferably used in combination with α-cyano-4-hydroxycinnamic acid.

摘要翻译： 本发明提供能够通过质谱法主要检测目标物质的方法，并且还可以在不进行富集过程的情况下有效地应用于要测量的样品。 一种质谱法，其特征在于，在通过使用比所述特定物质更可能离子化的基质更特别地电离含有特定物质的混合物样品和特定物质以外的物质的特定被测物质进行质谱分析 从特定物质以外的物质，选择性地测量混合物中的特定物质。 优选地，特定物质是用2-硝基苯硫酰氯修饰的肽，并且基质是硝基苯衍生物如羟基硝基苯甲酸。 该基质优选与α-氰基-4-羟基肉桂酸组合使用。

公开(公告)号：US20050224710A1

公开(公告)日：2005-10-13

申请号：US11028216

申请日：2005-01-04

申请人： Eiichi Matsuo ,  Makoto Watanabe ,  Noriyuki Ojima ,  Chikako Toda ,  Hiroki Kuyama ,  Osamu Nishimura

发明人： Eiichi Matsuo ,  Makoto Watanabe ,  Noriyuki Ojima ,  Chikako Toda ,  Hiroki Kuyama ,  Osamu Nishimura

IPC分类号： G01N33/68 ,  H01J49/16

CPC分类号： H01J49/164 ,  G01N33/6848

摘要： The present invention provides a method capable of efficiently ionizing hydrophobic peptides in MALDI-IT, MALDI-IT-TOF, and MALDI-FTICR mass spectrometers. A method of measuring a peptide with a mass spectrometer having a MALDI (Matrix Assisted Laser Desorption/Ionization) ion source, using α-cyano-3-hydroxycinnamic acid or 3-hydroxy-4-nitrobenzoic acid as a matrix. Preferably, a peptide derivatized with 2-nitrobenzenesulfenyl chloride is measured with a MALDI-IT, MALDI-IT-TOF, or MALDI-FTICR mass spectrometer. When 3-hydroxy-4-nitrobenzoic acid is used as a matrix, the matrix is preferably used as a mixed matrix in which α-cyano-4-hydroxycinnamic acid is combined.

摘要翻译： 本发明提供了能够有效地离子化MALDI-IT，MALDI-IT-TOF和MALDI-FTICR质谱仪中的疏水性肽的方法。 使用α-氰基-3-羟基肉桂酸或3-羟基-4-硝基苯甲酸作为基质的具有MALDI（Matrix Assisted Laser Desorption / Ionization）离子源的质谱仪测量肽的方法。 优选地，用MALDI-IT，MALDI-IT-TOF或MALDI-FTICR质谱仪测量用2-硝基苯硫基氯衍生的肽。 当使用3-羟基-4-硝基苯甲酸作为基质时，优选将基质用作混合基质，其中组合了α-氰基-4-羟基肉桂酸。