公开(公告)号：US20120135917A1

公开(公告)日：2012-05-31

申请号：US13377057

申请日：2010-06-16

申请人： Eisaku Yoshihara ,  Shigeki Mitsunaga ,  Hidetoshi Inoko

发明人： Eisaku Yoshihara ,  Shigeki Mitsunaga ,  Hidetoshi Inoko

IPC分类号： A61K38/07 ,  C07K7/06 ,  A61K38/08 ,  C07K5/10

CPC分类号： A61K45/06 ,  A61K38/07 ,  A61K38/08 ,  Y02A50/473 ,  Y02A50/481 ,  A61K2300/00

摘要： Provided are: a method for blocking the biosynthesis of an outer membrane protein (OMP) necessary for the survival of Gram-negative bacteria by inhibiting the formation of a YaeT complex in the outer membrane of the bacteria and an agent therefor for the purpose of basically solving a problem of the development of multidrug resistance in Gram-negative bacteria. Specifically disclosed is an anti-Gram-negative bacteria agent, wherein the agent exerts a bactericidal action, a growth-inhibiting action, and/or a drug efflux-inhibiting action on Gram-negative bacteria by inhibiting the formation of a YaeT complex. The agent is preferably a peptide molecule comprising an amino acid sequence consisting of at least LTLR or a peptide molecule comprising an amino acid sequence consisting of at least FIRL.

摘要翻译： 本发明提供：通过抑制细菌外膜中的YaeT复合物的形成和用于本发明的药剂的方法来阻断革兰氏阴性细菌的存活所需的外膜蛋白（OMP）的生物合成的方法 解决革兰氏阴性菌多药耐药发展的问题。 具体公开了抗革兰氏阴性细菌剂，其中该药物通过抑制YaeT复合物的形成而对革兰氏阴性细菌产生杀菌作用，生长抑制作用和/或药物流出抑制作用。 该试剂优选为包含至少由LTLR组成的氨基酸序列的肽分子或包含至少由FIRL组成的氨基酸序列的肽分子。

公开(公告)号：US20140206005A1

公开(公告)日：2014-07-24

申请号：US14233909

申请日：2012-05-18

申请人： Takashi Shiina ,  Shingo Suzuki ,  Yuki Ozaki ,  Shigeki Mitsunaga ,  Hidetoshi Inoko

发明人： Takashi Shiina ,  Shingo Suzuki ,  Yuki Ozaki ,  Shigeki Mitsunaga ,  Hidetoshi Inoko

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6888 ,  C12Q2600/156 ,  C12Q2600/16

摘要： The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing a set of primers which can respectively anneal specifically to an upstream region and a downstream region of each of HLA-A, HLA-B, HLA-C, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 gene in the nucleotide sequence for the human genome, and a set of primers which can respectively anneal specifically to exon-2 and a 3′-side non-translated region in HLA-DRB1; (2) a step of carrying out the PCR amplification of a sample to be tested (DNA) using the sets of primers; (3) a step of determining the nucleotide sequence for a PCR-amplified product; and (4) an optional step of carrying out the homology search in a data base.

摘要翻译： 本发明的目的是提供用于高精度DNA分型的方法和试剂盒，其中消除了从相位模糊性导出的歧义。 本发明提供HLA的DNA分型方法，其特征在于包括：（1）制备能够分别退火至HLA-A的上游区域和下游区域的一组引物的步骤， 人类基因组核苷酸序列中的HLA-B，HLA-C，HLA-DQA1，HLA-DQB1，HLA-DPA1和HLA-DPB1基因，以及一组可分别退火至外显子-2和3 “HLA-DRB1”非翻译区; （2）使用引物组进行待测试样品（DNA）的PCR扩增的步骤; （3）确定PCR扩增产物的核苷酸序列的步骤; 和（4）在数据库中执行同源性搜索的可选步骤。