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公开(公告)号：US20080113346A1
公开(公告)日：2008-05-15
申请号：US10593892
申请日：2005-03-29
申请人： Hidetoshi Inoko , Gen Tamiya , Takashi Gojobori
发明人： Hidetoshi Inoko , Gen Tamiya , Takashi Gojobori
CPC分类号： C12Q1/6883 , C07K14/4713 , C12Q2600/156 , C12Q2600/158
摘要： It is intended to identify rheumatoid arthritis susceptibility genes by a highly efficient, low-cost mapping method using microsatellites. In the present invention, novel rheumatoid arthritis susceptibility genes, that is, TNXB, NOTCH4, RAB6A, MPRL48, UCP2, and UCP3 genes, in the human genomic DNA sequence were identified by conducting case-control association analysis on rheumatoid arthritis by use of microsatellite polymorphic markers assigned at approximately 100-kb intervals to narrow down candidate regions and then conducting association analysis and linkage analysis with SNP as a marker.
摘要翻译： 旨在通过使用微卫星的高效，低成本的测绘方法鉴定类风湿关节炎易感基因。 在本发明中，通过使用微卫星对类风湿关节炎进行病例对照关联分析，鉴定了人类基因组DNA序列中的新型类风湿关节炎易感性基因，即TNXB，NOTCH4，RAB6A，MPRL48，UCP2和UCP3基因 以约100-kb间隔分配的多态标记物以缩小候选区域，然后以SNP作为标记进行缔合分析和连锁分析。
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公开(公告)号：US20120135917A1
公开(公告)日：2012-05-31
申请号：US13377057
申请日：2010-06-16
CPC分类号： A61K45/06 , A61K38/07 , A61K38/08 , Y02A50/473 , Y02A50/481 , A61K2300/00
摘要： Provided are: a method for blocking the biosynthesis of an outer membrane protein (OMP) necessary for the survival of Gram-negative bacteria by inhibiting the formation of a YaeT complex in the outer membrane of the bacteria and an agent therefor for the purpose of basically solving a problem of the development of multidrug resistance in Gram-negative bacteria. Specifically disclosed is an anti-Gram-negative bacteria agent, wherein the agent exerts a bactericidal action, a growth-inhibiting action, and/or a drug efflux-inhibiting action on Gram-negative bacteria by inhibiting the formation of a YaeT complex. The agent is preferably a peptide molecule comprising an amino acid sequence consisting of at least LTLR or a peptide molecule comprising an amino acid sequence consisting of at least FIRL.
摘要翻译： 本发明提供：通过抑制细菌外膜中的YaeT复合物的形成和用于本发明的药剂的方法来阻断革兰氏阴性细菌的存活所需的外膜蛋白（OMP）的生物合成的方法 解决革兰氏阴性菌多药耐药发展的问题。 具体公开了抗革兰氏阴性细菌剂，其中该药物通过抑制YaeT复合物的形成而对革兰氏阴性细菌产生杀菌作用，生长抑制作用和/或药物流出抑制作用。 该试剂优选为包含至少由LTLR组成的氨基酸序列的肽分子或包含至少由FIRL组成的氨基酸序列的肽分子。
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公开(公告)号：US06933118B2
公开(公告)日：2005-08-23
申请号：US10164230
申请日：2002-06-06
申请人： Hidetoshi Inoko , Gen Tamiya
发明人： Hidetoshi Inoko , Gen Tamiya
CPC分类号： C12Q1/6883 , C07K14/47 , C07K14/70539 , C12Q2600/156
摘要： By a detailed analysis of the sequences of the MHC S gene, SEEK1 gene, and HCR gene of Japanese patients with psoriasis and healthy individuals, it was demonstrated that some of the examined polymorphisms significantly correlate with psoriasis in the group of Japanese patients. Based on these correlations, it was demonstrated that psoriasis vulgaris can be detected by analyzing these gene polymorphisms in patients with psoriasis.
摘要翻译： 通过对日本银屑病和健康个体患者的MHC S基因，SEEK1基因和HCR基因序列的详细分析，证实了一些被检查的多态性与日本患者组中的牛皮癣显着相关。 基于这些相关性，已经证明通过分析牛皮癣患者的这些基因多态性可以检测到牛皮癣。
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公开(公告)号：US20140206005A1
公开(公告)日：2014-07-24
申请号：US14233909
申请日：2012-05-18
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6888 , C12Q2600/156 , C12Q2600/16
摘要： The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing a set of primers which can respectively anneal specifically to an upstream region and a downstream region of each of HLA-A, HLA-B, HLA-C, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 gene in the nucleotide sequence for the human genome, and a set of primers which can respectively anneal specifically to exon-2 and a 3′-side non-translated region in HLA-DRB1; (2) a step of carrying out the PCR amplification of a sample to be tested (DNA) using the sets of primers; (3) a step of determining the nucleotide sequence for a PCR-amplified product; and (4) an optional step of carrying out the homology search in a data base.
摘要翻译： 本发明的目的是提供用于高精度DNA分型的方法和试剂盒，其中消除了从相位模糊性导出的歧义。 本发明提供HLA的DNA分型方法，其特征在于包括：（1）制备能够分别退火至HLA-A的上游区域和下游区域的一组引物的步骤， 人类基因组核苷酸序列中的HLA-B，HLA-C，HLA-DQA1，HLA-DQB1，HLA-DPA1和HLA-DPB1基因，以及一组可分别退火至外显子-2和3 “HLA-DRB1”非翻译区; （2）使用引物组进行待测试样品（DNA）的PCR扩增的步骤; （3）确定PCR扩增产物的核苷酸序列的步骤; 和（4）在数据库中执行同源性搜索的可选步骤。
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5.发明申请公开(公告)号：US20070009515A1
公开(公告)日：2007-01-11
申请号：US10516209
申请日：2003-05-30
IPC分类号： A61K39/395 , C12Q1/68 , G01N33/567 , C07H21/04 , C12P21/06 , C07K14/705 , C07K16/28
CPC分类号： G01N33/6881 , C07K14/47 , C12Q1/6837 , C12Q1/6883 , C12Q2600/158 , G01N33/6893 , G01N2500/00 , G01N2800/20
摘要： The present inventors used microarrays to identify a novel gene from cDNA clones showing significantly different gene expression in normal tissues compared to psoriasis lesion tissues. This gene was named secretory Ly-6/uPAR-related protein-2 (SLURP-2). Quantitative real-time RT-PCR analysis using total RNA extract was used to compare SLURP-2 gene expression between psoriatic lesional skin, non-lesional skin, and normal skin, to indicate that the SLURP-2 gene is significantly upregulated in psoriasis lesions. Thus, SLURP-2 gene can be used as a diagnostic marker for inflammatory skin diseases such as psoriasis.
摘要翻译： 与银屑病病变组织相比，本发明人使用微阵列鉴定来自正常组织中显示显着不同的基因表达的cDNA克隆的新基因。 该基因命名为分泌型Ly-6 / uPAR相关蛋白-2（SLURP-2）。 使用总RNA提取物的定量实时RT-PCR分析用于比较牛皮癣损伤皮肤，非损伤皮肤和正常皮肤之间的SLURP-2基因表达，以表明在牛皮癣病变中SLURP-2基因显着上调。 因此，SLURP-2基因可用作炎性皮肤疾病如牛皮癣的诊断标记物。
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6.发明授权公开(公告)号：US07510834B2
公开(公告)日：2009-03-31
申请号：US10674124
申请日：2003-09-26
申请人： Hidetoshi Inoko , Gen Tamiya
发明人： Hidetoshi Inoko , Gen Tamiya
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/156 , C12Q2531/113
摘要： The present invention provides a gene mapping method which involves analysis of a DNA sample from a test subject and from a control subject for the presence of an allelic form of a plurality of microsatellite genetic polymorphism marker, which markers are located at intervals of about 50 Kb to 150 Kb on the human genome, in order to identify regions of the genome associated with a characteristic of the test subjects relative to the control subjects, e.g., a region containing a pathogenic gene or a gene relating to human phenotypes with genetic factors. The invention also features genomic regions so identified that are associated with susceptibility or the presence of psoriasis vulgaris and with rheumatoid arthritis.
摘要翻译： 本发明提供了一种基因作图方法，该方法包括从测试受试者和对照受试者分析存在多个微卫星遗传多态标志物的等位基因形式的DNA样品，该标记位于约50Kb的间隔 到人类基因组上的150Kb，以便鉴定与测试受试者相对于对照受试者的特征相关的基因组区域，例如含有病原基因的区域或与遗传因子的人表型相关的基因。 本发明还具有与易感性相关的基因组区域，或与寻常型牛皮癣和类风湿性关节炎的存在有关。
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7.发明申请Novel polymorphic microsatellite markers in the human MHC class II region 审中-公开人类MHC II类区域的新型多态性微卫星标记公开(公告)号：US20030108940A1
公开(公告)日：2003-06-12
申请号：US10314405
申请日：2002-12-06
申请人： Hidetoshi Inoko
发明人： Hidetoshi Inoko , Gen Tamiya , Yasunari Matsuzaka
IPC分类号： C12Q001/68 , C07H021/04
CPC分类号： C12Q1/6881 , C12Q2600/156
摘要： Novel polymorphic microsatellite markers in the human MHC class II region and methods for disease mapping and genotyping with said microsatellite markers are provided. Said microsatellite markers are useful in HLA-related research, such as genetic mapping of HLA class II associated diseases, transplantation matching, population genetics, and identification of recombination hot spots as well as linkage disequilibrium studies.
摘要翻译： 提供了人类MHC II类区域中的新型多态性微卫星标记和用所述微卫星标记进行疾病测定和基因分型的方法。 所述微卫星标记物可用于HLA相关研究，例如HLA II类相关疾病的遗传作图，移植匹配，群体遗传学，重组热点鉴定以及连锁不平衡研究。
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8.发明授权Antibodies produced from lipopolysaccharide-stimulated monocyte/macprophage cell lines 失效由脂多糖刺激的单核细胞/巨噬细胞系产生的抗体
公开(公告)号：US5677150A
公开(公告)日：1997-10-14
申请号：US603090
申请日：1996-02-20
申请人： Nobunao Ikewaki , Hidetoshi Inoko
发明人： Nobunao Ikewaki , Hidetoshi Inoko
IPC分类号： A61K39/395 , C07K16/28 , C12P21/08 , C07K16/18 , C12N5/12
CPC分类号： C07K16/28
摘要： Lipopolysaccharide-stimulated monocyte/macrophage cell lines are used as antigens to obtain antibodies. Antibodies which inhibit intercellular adhesion of cells stimulated by a differentiation factor or which induce intercellular aggregation of cells in the process of differentiation under stimulation by a differentiation factor are disclosed. Such antibodies can be used to suppress immune responses and in clinical diagnosis of immune system disorders.
摘要翻译： 脂多糖刺激的单核细胞/巨噬细胞系用作抗原以获得抗体。 公开了抑制由分化因子刺激的细胞的细胞间粘附或通过分化因子刺激的分化过程中诱导细胞的细胞间聚集的抗体。 这些抗体可用于抑制免疫应答和免疫系统疾病的临床诊断。
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