公开(公告)号：US20110020888A1

公开(公告)日：2011-01-27

申请号：US12518553

申请日：2007-12-14

申请人： Eleftherios Terry Papoutsakis ,  Sang Yup Lee ,  Jin Hwan Park

发明人： Eleftherios Terry Papoutsakis ,  Sang Yup Lee ,  Jin Hwan Park

IPC分类号： C12P7/16

CPC分类号： C12P7/16 ,  Y02E50/10

摘要： The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol.

摘要翻译： 本发明涉及使用能够以丁酰辅酶A作为中间体生物合成丁醇的细菌生产丁醇的方法。 更具体地，涉及一种生产丁醇的方法，该方法包括使用各种方法在含有编码AdhE的基因（负责将丁酰辅酶A转化为丁醇的酶）的细菌中生成副特异性辅酶A，并将丁酰辅酶A 变成丁醇。

公开(公告)号：US20100136640A1

公开(公告)日：2010-06-03

申请号：US12519060

申请日：2007-12-14

申请人： Sang Yup Lee ,  Jin Hwan Park ,  Eleftherios Terry Papoutsakis

发明人： Sang Yup Lee ,  Jin Hwan Park ,  Eleftherios Terry Papoutsakis

IPC分类号： C12P7/16 ,  C12N1/21 ,  C12N1/19 ,  C12N1/15

CPC分类号： C12P7/16 ,  Y02E50/10

摘要： The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified.

摘要翻译： 本发明涉及具有提高的丁醇生产能力的重组突变体微生物和使用其的丁醇生产方法。 在微生物中，编码负责乳酸盐，乙醇和/或乙酸盐的生物合成的酶的基因被缺失或减弱，并且引入并扩增了参与丁醇生物合成的酶编码基因。

公开(公告)号：US20100143985A1

公开(公告)日：2010-06-10

申请号：US12525403

申请日：2008-02-11

申请人： Sang Yup Lee ,  Eleftherios Terry Papoutsakis ,  Yu-Sin Jang

发明人： Sang Yup Lee ,  Eleftherios Terry Papoutsakis ,  Yu-Sin Jang

IPC分类号： C12P17/18 ,  C12N1/19 ,  C12P7/16

CPC分类号： C12P7/16 ,  C12N15/81 ,  Y02E50/10

摘要： Disclosed herein are a method for producing butanol in yeast having the ability to biosynthesize butanol using butyryl-CoA as an intermediate, the method comprises producing butyryl-CoA in yeast having a CoAT (acetyl-CoA:butyryl-CoA CoA-transferase)-encoding gene introduced thereinto, through various pathways, and then converting the produced butyryl-CoA to butanol.

摘要翻译： 本发明公开了一种使用丁酰辅酶A作为中间体生产丁醇生物合成能力的酵母中的丁醇生产方法，该方法包括在具有CoAT（乙酰辅酶A：丁酰辅酶A CoA转移酶）编码的酵母中制备丁酰辅酶A 基因通过各种途径导入其中，然后将生成的丁酰辅酶A转化为丁醇。