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    METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-CoA AS AN INTERMEDIATE USING BACTERIA
    1.
    发明申请
    METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-CoA AS AN INTERMEDIATE USING BACTERIA 审中-公开
    通过使用BACATIA的中间体通过丁二醇辅酶A制备丁醇的方法

    公开(公告)号：US20110020888A1

    公开(公告)日：2011-01-27

    申请号：US12518553

    申请日：2007-12-14

    申请人： Eleftherios Terry Papoutsakis ,  Sang Yup Lee ,  Jin Hwan Park

    发明人： Eleftherios Terry Papoutsakis ,  Sang Yup Lee ,  Jin Hwan Park

    IPC分类号： C12P7/16

    CPC分类号： C12P7/16 ,  Y02E50/10

    摘要： The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol.

    摘要翻译： 本发明涉及使用能够以丁酰辅酶A作为中间体生物合成丁醇的细菌生产丁醇的方法。 更具体地，涉及一种生产丁醇的方法，该方法包括使用各种方法在含有编码AdhE的基因（负责将丁酰辅酶A转化为丁醇的酶）的细菌中生成副特异性辅酶A，并将丁酰辅酶A 变成丁醇。

    L-Threonine Overproducing Microorganism and Method for Preparing L-Threonine Using the Same
    3.
    发明申请
    L-Threonine Overproducing Microorganism and Method for Preparing L-Threonine Using the Same 有权
    L-苏氨酸过量生产微生物及使用其制备L-苏氨酸的方法

    公开(公告)号：US20110003349A1

    公开(公告)日：2011-01-06

    申请号：US12160126

    申请日：2007-07-02

    申请人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

    发明人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

    IPC分类号： C12P13/08 ,  C12N15/63 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19

    CPC分类号： C12P13/08

    摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

    摘要翻译： 本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和 使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。 通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

    Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same
    4.
    发明授权
    Recombinant microorganisms having increased ability to produce butanol and method of producing butanol using the same 有权
    具有提高丁醇生产能力的重组微生物和使用其生产丁醇的方法

    公开(公告)号：US09096872B2

    公开(公告)日：2015-08-04

    申请号：US13497743

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    IPC分类号： C12N1/21 ,  C12P7/16 ,  C12N9/04 ,  C12N9/10

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

    摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

    摘要翻译： 本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。 更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。 在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。 通过操作微生物的代谢通过获得的重组微生物能够以高效率选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME
    5.
    发明申请
    MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 有权
    具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法

    公开(公告)号：US20090053779A1

    公开(公告)日：2009-02-26

    申请号：US12097099

    申请日：2007-03-14

    申请人： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    发明人： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    IPC分类号： C12P13/08 ,  C12N15/01 ,  C12N15/80 ,  C12N15/81 ,  C12N15/74 ,  C12N1/14 ,  C12N1/15 ,  C12N1/16 ,  C12N1/19 ,  C12N1/20 ,  C12N1/21

    CPC分类号： C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

    摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

    摘要翻译： 本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

    Recombinant microorganisms having enhanced propanol and method for preparing propanol using the same
    6.
    发明授权
    Recombinant microorganisms having enhanced propanol and method for preparing propanol using the same 有权
    具有增强丙醇的重组微生物和使用其制备丙醇的方法

    公开(公告)号：US08715976B2

    公开(公告)日：2014-05-06

    申请号：US13537988

    申请日：2012-06-29

    申请人： Sang Yup Lee ,  Yong Jun Choi ,  Jin Hwan Park

    发明人： Sang Yup Lee ,  Yong Jun Choi ,  Jin Hwan Park

    IPC分类号： C12P7/04

    CPC分类号： C12P7/04 ,  C12N15/52

    摘要： The present invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, and to a method of producing propanol using the same. More particularly, the invention relates to mutant microorganisms having the ability to produce propanol in high concentration and high yield, which have introduced therein genes that encodes enzymes which are involved in the biosynthesis of propanol from threonine, and to a method of producing propanol using the same.

    摘要翻译： 本发明涉及具有以高浓度和高收率生产丙醇的能力的突变微生物以及使用其制备丙醇的方法。 更具体地，本发明涉及具有以高浓度和高产率生产丙醇的能力的突变微生物，其中引入了编码参与苏氨酸生物合成丙醇的酶的基因，以及使用该方法生产丙醇的方法 相同。

    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME
    7.
    发明申请
    RECOMBINANT MICROORGANISMS HAVING INCREASED ABILITY TO PRODUCE BUTANOL AND METHOD OF PRODUCING BUTANOL USING THE SAME 有权
    具有生产丁醇的能力提高的重组微生物和使用其的丁醇的生产方法

    公开(公告)号：US20130017588A1

    公开(公告)日：2013-01-17

    申请号：US13497743

    申请日：2010-09-21

    申请人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    发明人： Sang Yup Lee ,  Yu-Sin Jang ,  Jin Young Lee ,  Joung Min Lee ,  Jin Hwan Park

    IPC分类号： C12N1/21 ,  C12P7/16 ,  C12N15/01

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y101/01001 ,  C12Y203/01008 ,  C12Y203/01019 ,  Y02E50/10

    摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

    摘要翻译： 本发明涉及生产丁醇的能力提高的重组微生物，以及使用其制备丁醇的方法。 更具体地，本发明涉及通过操作其代谢网络来增加生产丁醇的能力的重组微生物，以及使用其生产丁醇的方法。 在具有编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中，具有增加的产生丁醇能力的重组微生物包括编码转化乙酰CoA至乙酸的酶的基因的缺失。 通过操作微生物的代谢通过获得的重组微生物能够高效地选择性地生产丁醇，因此可用作生产工业溶剂和运输燃料的微生物。

    L-threonine overproducing microorganism and method for preparing L-threonine using the same
    8.
    发明授权
    L-threonine overproducing microorganism and method for preparing L-threonine using the same 有权
    L-苏氨酸过量生产微生物和使用其制备L-苏氨酸的方法

    公开(公告)号：US08852898B2

    公开(公告)日：2014-10-07

    申请号：US12160126

    申请日：2007-07-02

    申请人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

    发明人： Sang Yup Lee ,  Kwang Ho Lee ,  Jin Hwan Park ,  Tae Yong Kim

    IPC分类号： C12P13/08

    CPC分类号： C12P13/08

    摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

    摘要翻译： 本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和 使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。 通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

    Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same
    9.
    发明授权
    Mutant microorganism having improved production ability of branched amino acid and method for preparing branched amino acid using the same 有权
    具有提高支链氨基酸生产能力的突变体微生物及使用其制备支链氨基酸的方法

    公开(公告)号：US08148137B2

    公开(公告)日：2012-04-03

    申请号：US12097099

    申请日：2007-03-14

    申请人： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    发明人： Sang Yup Lee ,  Jin Hwan Park ,  Kwang Ho Lee ,  Tae Yong Kim

    IPC分类号： C12N1/21 ,  C12P13/08

    CPC分类号： C12P13/08 ,  C12N9/1014 ,  C12Y201/02011

    摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site-specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.

    摘要翻译： 本发明涉及具有提高的支链氨基酸生产率的突变微生物，以及使用突变微生物生产支链氨基酸的方法。 更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物 涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。 通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。