公开(公告)号：US20230392144A1

公开(公告)日：2023-12-07

申请号：US18328650

申请日：2023-06-02

Applicant: Element Biosciences, Inc.

Inventor： Andrew PRICE ,  Junhua ZHAO ,  Semyon KRUGLYAK ,  Kelly BLEASE ,  Hua YU ,  Andrew ALTOMARE ,  Ryan KELLEY ,  Juan MORENO

IPC: C12N15/10 ,  C12Q1/6834

CPC classification number: C12N15/1093 ,  C12Q1/6834

Abstract: The present disclosure provides compositions comprising reagents employed in a nucleic acid library preparation workflow for removing deaminated bases, and methods for using the reagents. The compositions and methods described herein reduce base call errors, such as C:G to T:A transitions, in nucleic acid sequencing workflows.

公开(公告)号：US20250146068A1

公开(公告)日：2025-05-08

申请号：US18919127

申请日：2024-10-17

Applicant: Element Biosciences, Inc.

Inventor： Tsung-Li LIU ,  Sinan ARSLAN ,  Michael PREVITE ,  Tuval BEN-YEHEZKEL ,  Connor THOMPSON ,  Matthew KELLINGER ,  Hui Zhen MAH ,  Molly HE ,  Andrew PRICE

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6841 ,  C12Q1/6844 ,  G01N33/58

Abstract: The present disclosure provides methods for conducting in situ multiplex and multi-omics detection and identification using coded padlocks probes. The methods comprise simultaneous use of RNA-specific padlock probes and polypeptide-specific padlock probes to detect both RNA and polypeptides in a cellular sample. Both types of probes include a barcode that unique identifies the RNA or polypeptide that that padlock probe detects. Both types of probes also include a batch-specific sequencing primer binding site to enable sequencing a desired subset of concatemer template molecules. Use of the batch-specific sequencing primers reduces overcrowding signals and images, to produces optical images that are intense and resolvable. By conducting multiple rounds of sequencing on the same cellular sample using different batch-specific sequencing primers enables multiplex and multi-omics sequencing to reveal numerous target RNAs and their encoded polypeptides.