公开(公告)号：US20250019760A1

公开(公告)日：2025-01-16

申请号：US18654808

申请日：2024-05-03

Applicant: Element Biosciences, Inc.

Inventor： William LIGHT ,  Hua YU ,  Junhua ZHAO ,  Su ZHANG ,  Samantha SNOW ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Marco TJIOE ,  Scott IM ,  James GHADIALI ,  Michael KIM ,  Hermes TAYLOR ,  Michael PREVITE ,  Jake LEVIEUX ,  Ramreddy TIPANNA ,  Molly HE

IPC: C12Q1/6874 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6876

Abstract: The present disclosure provides compositions comprising enzyme-based reagents, and methods using the enzyme-based reagents, for nucleic acid sequencing. The enzyme-based reagents efficiently remove sequencing read products from a first sequenced region of a template molecule, thereby reducing residual signals in a second sequenced region on the same template molecule.

公开(公告)号：US20240084380A1

公开(公告)日：2024-03-14

申请号：US18450302

申请日：2023-08-15

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Michael KIM ,  Ramreddy TIPANNA ,  Chunhong ZHOU ,  William LIGHT ,  Hua YU ,  Junhua ZHAO ,  Tsung-Li LIU

IPC: C12Q1/6874 ,  C12Q1/6844

CPC classification number: C12Q1/6874 ,  C12Q1/6844 ,  C12Q1/6806

Abstract: The present disclosure provides compositions and related methods, e.g., for preparing immobilized nucleic acid nanostructures using compaction oligonucleotides. In some embodiments, rolling circle amplification reaction can be conducted with compaction oligonucleotides on-support or in-solution to generate concatemer molecules having multiple copies of a polynucleotide unit arranged in tandem. Each polynucleotide unit comprises a sequence-of-interest and at least one universal adaptor sequence that binds one end of a compaction oligonucleotide. The 5′ and 3′ regions of the compaction oligonucleotide can hybridize to the concatemer to pull together distal portions of the concatemer causing compaction of the concatemer to form a nanostructure. Nanostructures having tighter size and shape compared to concatemers generated in the absence of the compaction oligonucleotides. The compact and stable characteristics of the nucleic acid nanostructures improves sequencing accuracy by increasing signal intensity and they retain their shape and size during multiple sequencing cycles.