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公开(公告)号：US11932886B2
公开(公告)日：2024-03-19
申请号：US16761785
申请日：2018-12-22
发明人： Haibin Chen , Yong Koy Bong , Juanjuan Wang , Baoqin Cai , Chuanyang Shang , Marco Bocola
CPC分类号： C12P13/001 , C12N9/1096 , C12N11/00 , C12N15/70
摘要： Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.
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公开(公告)号：US11999976B2
公开(公告)日：2024-06-04
申请号：US17290916
申请日：2019-12-11
发明人： Haibin Chen , Chuanyang Shang , Baoqin Cai , Marco Bocola , Shumin Shi , Ruimei Hong , Lei Sun , Yong Koy Bong
CPC分类号： C12N9/0006 , C12N11/02 , C12P7/22 , C12P7/62 , C12P41/002 , C12Y101/01184
摘要： Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions. The use of the engineered polypeptides of the present invention greatly simplifies the production process of chiral alcohol compounds, reduces the cost of production and the impact on the environment, and has good industrial application prospects.
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