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公开(公告)号：US11932886B2
公开(公告)日：2024-03-19
申请号：US16761785
申请日：2018-12-22
发明人： Haibin Chen , Yong Koy Bong , Juanjuan Wang , Baoqin Cai , Chuanyang Shang , Marco Bocola
CPC分类号： C12P13/001 , C12N9/1096 , C12N11/00 , C12N15/70
摘要： Provided are amino acid sequences of engineered transaminase polynucleotide that are useful for asymmetrically synthesizing chiral amine compounds, and its preparation process and reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered transaminase polypeptides, engineered host cells capable of expressing engineered transaminase polypeptides, and methods of producing chiral amine compounds using engineered transaminase polypeptides. Compared to other enzymes, the engineered transaminase polypeptides provided by this invention have better catalytic activity and stability, and are not inhibited by chiral amine products in the synthesis process. The use of the engineered polypeptides of the present invention for the preparation of chiral amine compound results in higher unit activity, and has good industrial application prospects.
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公开(公告)号：US11999976B2
公开(公告)日：2024-06-04
申请号：US17290916
申请日：2019-12-11
发明人： Haibin Chen , Chuanyang Shang , Baoqin Cai , Marco Bocola , Shumin Shi , Ruimei Hong , Lei Sun , Yong Koy Bong
CPC分类号： C12N9/0006 , C12N11/02 , C12P7/22 , C12P7/62 , C12P41/002 , C12Y101/01184
摘要： Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions. The use of the engineered polypeptides of the present invention greatly simplifies the production process of chiral alcohol compounds, reduces the cost of production and the impact on the environment, and has good industrial application prospects.
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公开(公告)号：US11512304B2
公开(公告)日：2022-11-29
申请号：US17045088
申请日：2019-04-01
发明人： Haibin Chen , Yong Koy Bong , Qing Xu , Dongxiao Hu , Chengxiao Zhang , Baoqin Cai
摘要： Provided herein are engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of amino acids such as L-tyrosine to produce tyramine or catalyzing the decarboxylation of L-DOPA to produce dopamine. Also provided are the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, recombinant host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing tyramine or dopamine using the engineered decarboxylase polypeptides. Compared to the wild type decarboxylase, the engineered polypeptide provided by this disclosure has better activity and/or stability. The use of the engineered polypeptides for the preparation of tyramine or dopamine reduces the production cost and has a good industrial application prospect.
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公开(公告)号：US11274287B2
公开(公告)日：2022-03-15
申请号：US16616370
申请日：2018-05-09
发明人： Haibin Chen , Yong Koy Bong , Baoqin Cai , Qing Xu , Tianran Shen , Ameng Zhou , Jiadong Yang , Zhuhong Yang , Yaoyao Ji , Yingxin Zhang
摘要： The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
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