公开(公告)号：US09052322B2

公开(公告)日：2015-06-09

申请号：US14177003

申请日：2014-02-10

申请人： Epitomics, Inc.

发明人： Guo-Liang Yu

IPC分类号： G01N33/53 ,  C07K16/00 ,  G01N33/577 ,  C07K16/28 ,  G01N33/68 ,  G01N33/566

CPC分类号： G01N33/577 ,  C07K16/00 ,  C07K16/2863 ,  C07K2317/10 ,  C07K2317/56 ,  C07K2317/76 ,  C12Q1/6876 ,  G01N33/566 ,  G01N33/6854

摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

摘要翻译： 在某些实施方案中，该方法可以包括：a）从B细胞群获得抗体序列; b）将抗体序列分组以提供多组谱系相关抗体; c）在生物测定中测试来自每个组的单一抗体，并且在鉴定出第一抗体后，d）在第二生物测定中测试与第一抗体在同一组中的另外的抗体。 在另一个实施方案中，该方法可以包括：a）测试从动物的产生抗体的器官的第一部分获得的多个抗体; b）获得第一鉴定抗体的序列; c）从所述抗体产生器官的第二部分获得与所述第一抗体谱系相关的其它抗体的序列; 和c）在第二生物测定中测试另外的抗体。

公开(公告)号：US09696314B2

公开(公告)日：2017-07-04

申请号：US14973523

申请日：2015-12-17

申请人： Epitomics, Inc.

发明人： Guo-Liang Yu

IPC分类号： C07K16/00 ,  G01N33/53 ,  G01N33/577 ,  C07K16/28 ,  G01N33/68 ,  G01N33/566 ,  C12Q1/68

CPC分类号： G01N33/577 ,  C07K16/00 ,  C07K16/2863 ,  C07K2317/10 ,  C07K2317/56 ,  C07K2317/76 ,  C12Q1/6876 ,  G01N33/566 ,  G01N33/6854

摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

公开(公告)号：US09250244B2

公开(公告)日：2016-02-02

申请号：US14183075

申请日：2014-02-18

申请人： Epitomics, Inc. (c/o Abcam plc)

发明人： Guo-Liang Yu

IPC分类号： C07K16/00 ,  G01N33/53 ,  G01N33/577 ,  C07K16/28 ,  G01N33/68 ,  G01N33/566

CPC分类号： G01N33/577 ,  C07K16/00 ,  C07K16/2863 ,  C07K2317/10 ,  C07K2317/56 ,  C07K2317/76 ,  C12Q1/6876 ,  G01N33/566 ,  G01N33/6854

摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.

公开(公告)号：US08969013B2

公开(公告)日：2015-03-03

申请号：US13748507

申请日：2013-01-23

申请人： EPITOMICS, Inc.

发明人： Guo-Liang Yu

IPC分类号： C07K16/00 ,  G01N33/53 ,  G01N33/577 ,  C07K16/28 ,  G01N33/68 ,  G01N33/566

CPC分类号： G01N33/577 ,  C07K16/00 ,  C07K16/2863 ,  C07K2317/10 ,  C07K2317/56 ,  C07K2317/76 ,  C12Q1/6876 ,  G01N33/566 ,  G01N33/6854

摘要： In certain embodiments, the method may comprise: a) obtaining the antibody sequences from a population of B cells; b) grouping the antibody sequences to provide a plurality of groups of lineage-related antibodies; c) testing a single antibody from each of the groups in a bioassay and, after the first antibody has been identified, d) testing further antibodies that are in the same group as the first antibody in a second bioassay. In another embodiment, the method may comprise: a) testing a plurality of antibodies obtained from a first portion of an antibody producing organ of an animal; b) obtaining the sequence of a first identified antibody; c) obtaining from a second portion of said antibody producing organ the sequences of further antibodies that are related by lineage to said first antibody; and, c) testing the further antibodies in a second bioassay.