公开(公告)号：US20200224245A1

公开(公告)日：2020-07-16

申请号：US16631603

申请日：2018-07-18

Applicant: Exosome Diagnostics, Inc.

Inventor： Robert KITCHEN ,  Michael VALENTINO ,  Johan SKOG ,  Vasisht TADIGOTLA ,  Dalin CHAN ,  Sudipto CHAKRABORTTY ,  James HURLEY

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/6851

Abstract: The present disclosure relates to methods for providing a clinical assessment of a subject in need thereof using the gene expression level measurements from exosomes derived from the subject.

公开(公告)号：US20200208213A1

公开(公告)日：2020-07-02

申请号：US16342572

申请日：2017-10-23

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan SKOG ,  Sudipto CHAKRABORTTY ,  Dalin CHAN ,  Michael VALENTINO ,  Vasisht TADIGOTLA ,  Robert KITCHEN ,  Dominik GRIMM ,  Wei YU

IPC: C12Q1/6874 ,  G01N1/28 ,  C12N15/10 ,  G01N33/543

Abstract: The invention provides a series of steps that prepare nucleic acids (RNA and/or DNA) isolated from extracellular vesicles for sequencing. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected. These can then be used to identify various attributes such as gene expression, alternative splicing, and the detection of both somatic and germline mutations including single nucleotide variants (SNV) and structural variations (insertions/deletions, fusions, inversions).