公开(公告)号：US20130109098A1

公开(公告)日：2013-05-02

申请号：US13808285

申请日：2011-07-06

申请人： F.C. Thomas Allnutt ,  Bradley Lynn Postier ,  Richard T. Sayre ,  Daniel A. Coury ,  Anil Kumar ,  Andrew Swanson ,  Mark Scott Abad ,  Zoee Gokhale Perrine

发明人： F.C. Thomas Allnutt ,  Bradley Lynn Postier ,  Richard T. Sayre ,  Daniel A. Coury ,  Anil Kumar ,  Andrew Swanson ,  Mark Scott Abad ,  Zoee Gokhale Perrine

IPC分类号： C12N15/79

CPC分类号： C12N15/79 ,  C12N1/12

摘要： Biosecure algae and methods for preparing biosecure algae that have a substantially decreased capability to survive in a natural environment are described. The methods include transforming a genetically modified alga to include an essential gene that is operably linked to a promoter system that is active only in the presence of an inducer compound, transforming the genetically modified alga to include a lethal gene that is operably linked with a promoter system that is inactive only in the presence of a repressor compound. The biosecure algae are only able to survive in an artificial algae culture that includes factors or conditions not found in a natural environment.

摘要翻译： 描述了生物安全藻类和用于制备在自然环境中具有显着降低的生存能力的生物安全藻类的方法。 所述方法包括转化遗传修饰的藻类以包括可操作地连接到仅在诱导物化合物存在下仅起作用的启动子系统的必需基因，将所述遗传修饰的藻类转化为包括与启动子可操作地连接的致死基因 仅在存在阻遏物化合物时不起作用的系统。 生物安全藻类只能在人造藻类培养物中生存，其中包括在自然环境中未发现的因素或条件。

公开(公告)号：US20120156717A1

公开(公告)日：2012-06-21

申请号：US13392950

申请日：2010-08-30

申请人： F.C. Thomas Allnutt ,  Bradley Lynn Postier ,  Richard T. Sayre ,  Daniel A. Coury

发明人： F.C. Thomas Allnutt ,  Bradley Lynn Postier ,  Richard T. Sayre ,  Daniel A. Coury

IPC分类号： C12P1/00 ,  C12P9/00 ,  C12P33/00 ,  C12N1/13 ,  C12P7/64

CPC分类号： C12P7/649 ,  Y02E50/13

摘要： Recombinant oleaginous alga that include one or more heterologous genes that increase the ability of the alga to use one or more natural saccharides such as cellulosic or hemicellulosic sugars for algal growth are described. The recombinant oleaginous algae are transformed to include one or more genes expressing sugar metabolizing enzymes or sugar transporting proteins, along with suitable control elements. Use of natural saccharides as a carbon source can allow the algae to produce biofuel precursors in a relatively efficient manner. Processes for preparing the alga, growing the alga, and extracting the biofuel precursors from the alga are also described.

摘要翻译： 描述了包含增加藻类使用一种或多种天然糖如纤维素或半纤维素糖用于藻类生长的能力的一种或多种异源基因的重组含油藻。 将重组含油藻类转化为包括一个或多个表达糖代谢酶或糖转运蛋白的基因以及合适的控制元件。 使用天然糖作为碳源可以使藻类以相对有效的方式生产生物燃料前体。 还描述了用于制备藻类，生长藻类以及从藻类中提取生物燃料前体的方法。