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公开(公告)号：US20110269225A1
公开(公告)日：2011-11-03
申请号：US13176026
申请日：2011-07-05
申请人： ARUN K. DHAR , F.C. THOMAS ALLNUTT
发明人： ARUN K. DHAR , F.C. THOMAS ALLNUTT
IPC分类号： C12N15/85
CPC分类号： C12N15/85 , A01K2227/70 , A01K2267/0393 , C12N2799/021 , C12N2800/90
摘要： Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picomavirus, such as a cricket paralysis-like picomavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
摘要翻译： 公开了一种或多种虾，贝类，软体动物和鱼的遗传操作的方法和构造。 核酸构建体包括一种启动子和一种诸如板球麻痹状皮莫瓦病毒之类的皮肤微病毒病毒的内部核糖体进入位点。 一个或多个开放阅读框可以与启动子和内部核糖体进入位点中的一个或两个可操作地相关联，并且一个或多个蛋白质或蛋白质亚基可以在将构建体引入宿主细胞时表达，例如进入虾 。 还描述了使用源自虾和/或虾病毒的增强子元件生产永生化甲壳类细胞系的方法。
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公开(公告)号：US20090258396A1
公开(公告)日：2009-10-15
申请号：US12489905
申请日：2009-06-23
CPC分类号： C07K14/005 , A01K2227/70 , A01K2267/01 , A61K2039/5256 , C07K14/505 , C07K14/62 , C07K16/00 , C07K2317/10 , C12N7/00 , C12N9/16 , C12N15/86 , C12N2710/14043 , C12N2710/18043 , C12N2750/14043 , C12N2770/16022 , C12N2770/16034 , C12N2770/22043 , C12N2770/36143 , C12N2830/00 , C12N2830/75
摘要： A crustacean or rotifer is infected with a recombinant infectious virus that expresses a protein exogenous to the virus. The genome of the crustacean or rotifer itself remains unaltered. Crustacean, rotifer, insect, or viral promoters drive the transcription of a gene inserted into the recombinant virus genome, and the virus replicates in the crustacean or rotifer cell cytoplasm. The infected crustacean or rotifer can be provided directly to humans or non-human animals, or, following production and harvest of the crustaceans or rotifers, purified recombinant protein or polypeptide can be provided. Large quantities of biopharmaceuticals can be produced rapidly and inexpensively using this production system.
摘要翻译： 用表达外源蛋白质的病毒的重组感染性病毒感染甲壳类动物或轮虫。 甲壳类或轮虫本身的基因组本身保持不变。 甲壳动物，轮虫，昆虫或病毒启动子驱动插入重组病毒基因组中的基因的转录，并且病毒在甲壳类或轮状细胞细胞质中复制。 受感染的甲壳动物或轮虫可以直接提供给人类或非人动物，或者在甲壳类动物或轮虫的生产和收获后，可提供纯化的重组蛋白或多肽。 可以使用该生产系统快速且廉价地生产大量的生物药物。
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3.发明申请公开(公告)号：US20120156717A1
公开(公告)日：2012-06-21
申请号：US13392950
申请日：2010-08-30
摘要： Recombinant oleaginous alga that include one or more heterologous genes that increase the ability of the alga to use one or more natural saccharides such as cellulosic or hemicellulosic sugars for algal growth are described. The recombinant oleaginous algae are transformed to include one or more genes expressing sugar metabolizing enzymes or sugar transporting proteins, along with suitable control elements. Use of natural saccharides as a carbon source can allow the algae to produce biofuel precursors in a relatively efficient manner. Processes for preparing the alga, growing the alga, and extracting the biofuel precursors from the alga are also described.
摘要翻译： 描述了包含增加藻类使用一种或多种天然糖如纤维素或半纤维素糖用于藻类生长的能力的一种或多种异源基因的重组含油藻。 将重组含油藻类转化为包括一个或多个表达糖代谢酶或糖转运蛋白的基因以及合适的控制元件。 使用天然糖作为碳源可以使藻类以相对有效的方式生产生物燃料前体。 还描述了用于制备藻类，生长藻类以及从藻类中提取生物燃料前体的方法。
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4.发明申请ANTIGENICITY OF INFECTIOUS PANCREATIC NECROSIS VIRUS VP2 SUB-VIRAL PARTICLES EXPRESSED IN YEAST 有权感染性胰腺炎病毒VP2亚历山大小肠病毒的抗生素公开(公告)号：US20100092521A1
公开(公告)日：2010-04-15
申请号：US12519991
申请日：2007-12-18
CPC分类号： A61K39/12 , A61K2039/51 , A61K2039/5258 , A61K2039/542 , A61K2039/552 , C07K14/005 , C12N7/00 , C12N2720/10022 , C12N2720/10023 , C12N2720/10034
摘要： Infectious pancreatic necrosis virus (IPNV), the etiologic agent of infectious pancreatic necrosis in salmonid fish, causes significant losses to the aquaculture industry. The gene for the viral capsid protein (VP2) was cloned into a yeast expression vector and expressed in Saccharomyces cerevisae. Expression of the capsid gene in yeast resulted in formation of approximately 20 nanometer sub-viral particles composed solely of VP2 protein. Anti-IPNV antibodies were detected in rainbow trout vaccinated either by injection of purified VP2-subviral particles (rVP2-SVP) or by feeding recombinant yeast expressing rVP2-SVP. Challenge of rVP2-SVP immunized trout with a heterologous IPNV strain and subsequent viral load determination showed that both injection and orally vaccinated fish had lower IPNV loads than naive or sham-vaccinated fish. This study demonstrates the ability of rVP2-SVPs to induce a specific immune response and the ability of immunized fish to reduce the viral load after an experimentally induced IPNV infection. The invention is not limited to IPNV, and is applicable to other similar viruses for which SVPs can be made and administered to fish.
摘要翻译： 传染性胰腺坏死病毒（IPNV）是鲑鱼感染性胰腺坏死的病原体，对水产养殖业造成重大损失。 将病毒衣壳蛋白（VP2）的基因克隆到酵母表达载体中并在酿酒酵母中表达。 酵母中衣壳基因的表达导致形成仅由VP2蛋白组成的约20纳米亚病毒颗粒。 通过注射纯化的VP2-亚病毒颗粒（rVP2-SVP）或通过加入表达rVP2-SVP的重组酵母，在虹鳟中检测到抗IPNV抗体。 用异源IPNV株进行rVP2-SVP免疫鳟鱼的挑战，随后进行病毒载量测定，结果表明注射和口服接种的鱼类比初始或假手术的鱼具有较低的IPNV负荷。 该研究证明rVP2-SVP诱导特异性免疫应答的能力以及免疫鱼在实验诱导的IPNV感染后降低病毒载量的能力。 本发明不限于IPNV，适用于可以制造和施用SVP的其他类似病毒。
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5.发明申请TROPHIC CONVERSION OF OBLIGATE PHOTOTROPHIC ALGAE THROUGH METABOLIC ENGINEERING 失效通过代谢工程进行的白藜芦醇的热转化公开(公告)号：US20080138851A1
公开(公告)日：2008-06-12
申请号：US11842898
申请日：2007-08-21
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译： 大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。 在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。 人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。 这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。 由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。 最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。
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公开(公告)号：US20130109098A1
公开(公告)日：2013-05-02
申请号：US13808285
申请日：2011-07-06
申请人： F.C. Thomas Allnutt , Bradley Lynn Postier , Richard T. Sayre , Daniel A. Coury , Anil Kumar , Andrew Swanson , Mark Scott Abad , Zoee Gokhale Perrine
发明人： F.C. Thomas Allnutt , Bradley Lynn Postier , Richard T. Sayre , Daniel A. Coury , Anil Kumar , Andrew Swanson , Mark Scott Abad , Zoee Gokhale Perrine
IPC分类号： C12N15/79
摘要： Biosecure algae and methods for preparing biosecure algae that have a substantially decreased capability to survive in a natural environment are described. The methods include transforming a genetically modified alga to include an essential gene that is operably linked to a promoter system that is active only in the presence of an inducer compound, transforming the genetically modified alga to include a lethal gene that is operably linked with a promoter system that is inactive only in the presence of a repressor compound. The biosecure algae are only able to survive in an artificial algae culture that includes factors or conditions not found in a natural environment.
摘要翻译： 描述了生物安全藻类和用于制备在自然环境中具有显着降低的生存能力的生物安全藻类的方法。 所述方法包括转化遗传修饰的藻类以包括可操作地连接到仅在诱导物化合物存在下仅起作用的启动子系统的必需基因，将所述遗传修饰的藻类转化为包括与启动子可操作地连接的致死基因 仅在存在阻遏物化合物时不起作用的系统。 生物安全藻类只能在人造藻类培养物中生存，其中包括在自然环境中未发现的因素或条件。
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7.发明申请Trophic Conversion of Obligate Phototrophic Algae Through Metabolic Engineering 审中-公开通过代谢工程营养转化特种光营养藻类公开(公告)号：US20120034653A1
公开(公告)日：2012-02-09
申请号：US13188806
申请日：2011-07-22
IPC分类号： C12P21/00 , C12N15/79 , C12N1/13 , C07C29/74 , C11B1/10 , C07C35/21 , C07C7/00 , C07K14/405 , A23K1/00 , C07K1/14
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译： 大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。 在这项研究中，显示微藻Phaeodaclylurn三角褐豆可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。 人和小球藻凯斯勒葡萄糖转运蛋白都促进了三角褐指藻的葡萄糖摄取，使得细胞代谢外源有机碳，并且独立于光。 这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。 由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。 最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。
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8.发明授权
公开(公告)号：US5741713A
公开(公告)日：1998-04-21
申请号：US667723
申请日：1996-06-21
摘要： Combinatorial libraries of labeled biochemical compounds and methods for producing such combinatorial libraries comprising the steps of producing labeled individual units, combining at least two of the labeled individual units so as to produce a labeled biochemical compound, and repeating this process at least once so as to produce a combinatorial library of labeled biochemical compounds. Also, methods for determining the conformation of a biochemical compound which comprise producing a combinatorial library of labeled biochemical compounds, contacting the combinatorial library of labeled biochemical compounds with a target receptor molecule so that a selected labeled biochemical compound binds to the target receptor molecule, and determining the conformation of the selected labeled biochemical compound when bound to the receptor molecule.
摘要翻译： 标记的生物化学化合物的组合文库和用于生产这种组合文库的方法，其包括以下步骤：产生标记的单个单元，将至少两个标记的单个单元组合以产生标记的生物化学化合物，并重复该过程至少一次，以便 生成标记生物化学化合物的组合文库。 另外，用于测定生物化学化合物构象的方法，其包括产生标记的生物化学化合物的组合文库，使标记的生物化学化合物的组合文库与靶受体分子接触，使选定的标记的生物化学化合物与靶受体分子结合，以及 当与受体分子结合时，确定选择的标记生物化学化合物的构象。
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