公开(公告)号：US11932894B2

公开(公告)日：2024-03-19

申请号：US17673465

申请日：2022-02-16

Applicant: FIRMENICH SA

Inventor： Michel Schalk ,  Pauline Anziani ,  Christian Goerner ,  Daniel Solis Escalante

IPC: C12P7/02 ,  C12N9/88

CPC classification number: C12P7/02 ,  C12N9/88 ,  C12Y402/03

Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide including a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.

公开(公告)号：US20230148463A9

公开(公告)日：2023-05-11

申请号：US17673465

申请日：2022-02-16

Applicant: FIRMENICH SA

Inventor： Michel Schalk ,  Pauline Anziani ,  Christian Goerner ,  Daniel Solis Escalante

IPC: C12P7/02 ,  C12N9/88

CPC classification number: C12P7/02 ,  C12N9/88 ,  C12Y402/03

Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide including a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.

公开(公告)号：US20230183761A1

公开(公告)日：2023-06-15

申请号：US17596878

申请日：2020-07-08

Applicant: Firmenich SA

Inventor： Michel Schalk ,  Fabienne Deguerry ,  Daniel Solis Escalante ,  Pauline Anziani ,  Sabine Beccucci

IPC: C12P7/62 ,  C12N9/02 ,  C12N9/18

CPC classification number: C12P7/62 ,  C12N9/0069 ,  C12Y113/00 ,  C12N9/18

Abstract: Described herein are biocatalytic methods of producing terpene degradation products useful as starting material for the production of perfumery ingredients, such as, for example, ambrox. In particular novel terpene degrading polypeptides (enal-cleaving polypeptides) and novel peptides converting terpenes compounds to oxygenated derivatives (oxygenases) and mutants and variants derived therefrom are described which may be applied in novel types of fully enzymatic multistep degradation pathways allowing the controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. Said novel biosynthetic strategies allow the fully biochemical synthesis of valuable terpene-derived compounds, like for example manooloxy or gamma ambrol. Also described herein are recombinant host organisms carrying the required set of genetic information for the functional expression of the set of enzymes necessary for catalyzing the combination of enzymatic conversion and degradation steps.

公开(公告)号：US20220186265A1

公开(公告)日：2022-06-16

申请号：US17673465

申请日：2022-02-16

Applicant: FIRMENICH SA

Inventor： Michel Schalk ,  Pauline Anziani ,  Christian Goerner ,  Daniel Solis Escalante

IPC: C12P7/02 ,  C12N9/88

Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide including a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.

公开(公告)号：US11293037B2

公开(公告)日：2022-04-05

申请号：US16618737

申请日：2018-05-31

Applicant: FIRMENICH SA

Inventor： Michel Schalk ,  Pauline Anziani ,  Christian Goerner ,  Daniel Solis Escalante

IPC: C12P7/02 ,  C12N9/88

Abstract: Described herein is a method of producing a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof by contacting at least one polypeptide with farnesyl diphosphate (FPP) with a polypeptide comprising a Haloacid dehalogenase (HAD)-like hydrolase domain and having bifunctional terpene synthase activity. The method may be performed in vitro or in vivo. Also described herein are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides described. The described method further provides host cells or organisms genetically modified to express the polypeptides and useful to produce a drimane sesquiterpene such as albicanol, drimenol and/or derivatives thereof.